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The association between interleukin-4 -590C/T genetic polymorphism, IL-4 serum level, and advanced endometriosis
AIM OF THE STUDY: Aim of the study was to investigate interleukin (IL)-4 serum levels in patients with advanced endometriosis and whether IL-4 promoter region (-590C/T) genetic polymorphism is involved in genetic susceptibility to endometriosis. MATERIAL AND METHODS: IL-4 serum levels and IL-4 -590C...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Polish Society of Experimental and Clinical Immunology
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4967651/ https://www.ncbi.nlm.nih.gov/pubmed/27536203 http://dx.doi.org/10.5114/ceji.2016.60992 |
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author | Malutan, Andrei M. Drugan, Cristina Drugan, Tudor Ciortea, Razvan Mihu, Dan |
author_facet | Malutan, Andrei M. Drugan, Cristina Drugan, Tudor Ciortea, Razvan Mihu, Dan |
author_sort | Malutan, Andrei M. |
collection | PubMed |
description | AIM OF THE STUDY: Aim of the study was to investigate interleukin (IL)-4 serum levels in patients with advanced endometriosis and whether IL-4 promoter region (-590C/T) genetic polymorphism is involved in genetic susceptibility to endometriosis. MATERIAL AND METHODS: IL-4 serum levels and IL-4 -590C/T genetic polymorphism were determined for 80 patients with advanced endometriosis and 85 healthy fertile women using a multiplex cytokine kit, with a Luminex 200 system; high molecular weight genomic DNA was extracted from peripheral blood leukocytes, and further analyzed by PCR amplification and restriction fragment length polymorphism (PCR-PFLP). The relationship between IL-4 serum levels, genotypes and haplotypes and the presence of endometriosis was explored. RESULTS: Interleukin 4 serum levels were significantly higher in the endometriosis group compared to controls (138,459 compared to 84,710, p < 0.001). No significant difference was observed in IL-4 serum levels between genotypes. There were no differences in IL-4 -590C/T genotypes and allele frequencies between control women and patients with endometriosis (χ(2) = 0.496, and χ(2) = 0.928, OR = 1.3636, CI: 0.725-2.564). CONCLUSIONS: The results suggest that in patients with advanced stages of endometriosis there is a higher serum level of IL-4, and that this value, or the presence of the disease, is not influenced by the presence of IL-4 -590C/T genetic polymorphism. |
format | Online Article Text |
id | pubmed-4967651 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Polish Society of Experimental and Clinical Immunology |
record_format | MEDLINE/PubMed |
spelling | pubmed-49676512016-08-17 The association between interleukin-4 -590C/T genetic polymorphism, IL-4 serum level, and advanced endometriosis Malutan, Andrei M. Drugan, Cristina Drugan, Tudor Ciortea, Razvan Mihu, Dan Cent Eur J Immunol Clinical Immunology AIM OF THE STUDY: Aim of the study was to investigate interleukin (IL)-4 serum levels in patients with advanced endometriosis and whether IL-4 promoter region (-590C/T) genetic polymorphism is involved in genetic susceptibility to endometriosis. MATERIAL AND METHODS: IL-4 serum levels and IL-4 -590C/T genetic polymorphism were determined for 80 patients with advanced endometriosis and 85 healthy fertile women using a multiplex cytokine kit, with a Luminex 200 system; high molecular weight genomic DNA was extracted from peripheral blood leukocytes, and further analyzed by PCR amplification and restriction fragment length polymorphism (PCR-PFLP). The relationship between IL-4 serum levels, genotypes and haplotypes and the presence of endometriosis was explored. RESULTS: Interleukin 4 serum levels were significantly higher in the endometriosis group compared to controls (138,459 compared to 84,710, p < 0.001). No significant difference was observed in IL-4 serum levels between genotypes. There were no differences in IL-4 -590C/T genotypes and allele frequencies between control women and patients with endometriosis (χ(2) = 0.496, and χ(2) = 0.928, OR = 1.3636, CI: 0.725-2.564). CONCLUSIONS: The results suggest that in patients with advanced stages of endometriosis there is a higher serum level of IL-4, and that this value, or the presence of the disease, is not influenced by the presence of IL-4 -590C/T genetic polymorphism. Polish Society of Experimental and Clinical Immunology 2016-07-15 2016 /pmc/articles/PMC4967651/ /pubmed/27536203 http://dx.doi.org/10.5114/ceji.2016.60992 Text en Copyright: © 2016 Polish Society of Experimental and Clinical Immunology http://creativecommons.org/licenses/by-nc-sa/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) License, allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material, provided the original work is properly cited and states its license. |
spellingShingle | Clinical Immunology Malutan, Andrei M. Drugan, Cristina Drugan, Tudor Ciortea, Razvan Mihu, Dan The association between interleukin-4 -590C/T genetic polymorphism, IL-4 serum level, and advanced endometriosis |
title | The association between interleukin-4 -590C/T genetic polymorphism, IL-4 serum level, and advanced endometriosis |
title_full | The association between interleukin-4 -590C/T genetic polymorphism, IL-4 serum level, and advanced endometriosis |
title_fullStr | The association between interleukin-4 -590C/T genetic polymorphism, IL-4 serum level, and advanced endometriosis |
title_full_unstemmed | The association between interleukin-4 -590C/T genetic polymorphism, IL-4 serum level, and advanced endometriosis |
title_short | The association between interleukin-4 -590C/T genetic polymorphism, IL-4 serum level, and advanced endometriosis |
title_sort | association between interleukin-4 -590c/t genetic polymorphism, il-4 serum level, and advanced endometriosis |
topic | Clinical Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4967651/ https://www.ncbi.nlm.nih.gov/pubmed/27536203 http://dx.doi.org/10.5114/ceji.2016.60992 |
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