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Effects of Erythropoietin Administration on Adrenal Glands of Landrace/Large White Pigs after Ventricular Fibrillation

Aim. To evaluate the effects of erythropoietin administration on the adrenal glands in a swine model of ventricular fibrillation and resuscitation. Methods. Ventricular fibrillation was induced via pacing wire forwarded into the right ventricle in 20 female Landrace/Large White pigs, allocated into...

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Autores principales: Faa, Armando, Faa, Gavino, Papalois, Apostolos, Obinu, Eleonora, Locci, Giorgia, Pais, Maria Elena, Lelovas, Pavlos, Barouxis, Dimitrios, Pantazopoulos, Charalampos, Vasileiou, Panagiotis V., Iacovidou, Nicoletta, Xanthos, Theodoros
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4967690/
https://www.ncbi.nlm.nih.gov/pubmed/27504455
http://dx.doi.org/10.1155/2016/7261960
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author Faa, Armando
Faa, Gavino
Papalois, Apostolos
Obinu, Eleonora
Locci, Giorgia
Pais, Maria Elena
Lelovas, Pavlos
Barouxis, Dimitrios
Pantazopoulos, Charalampos
Vasileiou, Panagiotis V.
Iacovidou, Nicoletta
Xanthos, Theodoros
author_facet Faa, Armando
Faa, Gavino
Papalois, Apostolos
Obinu, Eleonora
Locci, Giorgia
Pais, Maria Elena
Lelovas, Pavlos
Barouxis, Dimitrios
Pantazopoulos, Charalampos
Vasileiou, Panagiotis V.
Iacovidou, Nicoletta
Xanthos, Theodoros
author_sort Faa, Armando
collection PubMed
description Aim. To evaluate the effects of erythropoietin administration on the adrenal glands in a swine model of ventricular fibrillation and resuscitation. Methods. Ventricular fibrillation was induced via pacing wire forwarded into the right ventricle in 20 female Landrace/Large White pigs, allocated into 2 groups: experimental group treated with bolus dose of erythropoietin (EPO) and control group which received normal saline. Cardiopulmonary resuscitation (CPR) was performed immediately after drug administration as per the 2010 European Resuscitation Council (ERC) guidelines for Advanced Life Support (ALS) until return of spontaneous circulation (ROSC) or death. Animals who achieved ROSC were monitored, mechanically ventilated, extubated, observed, and euthanized. At necroscopy, adrenal glands samples were formalin-fixed, paraffin-embedded, and routinely processed. Sections were stained with hematoxylin-eosin. Results. Oedema and apoptosis were the most frequent histological changes and were detected in all animals in the adrenal cortex and in the medulla. Mild and focal endothelial lesions were also detected. A marked interindividual variability in the degree of the intensity of apoptosis and oedema at cortical and medullary level was observed within groups. Comparing the two groups, higher levels of pathological changes were detected in the control group. No significant difference between the two groups was observed regarding the endothelial changes. Conclusions. In animals exposed to ventricular fibrillation, EPO treatment has protective effects on the adrenal gland.
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spelling pubmed-49676902016-08-08 Effects of Erythropoietin Administration on Adrenal Glands of Landrace/Large White Pigs after Ventricular Fibrillation Faa, Armando Faa, Gavino Papalois, Apostolos Obinu, Eleonora Locci, Giorgia Pais, Maria Elena Lelovas, Pavlos Barouxis, Dimitrios Pantazopoulos, Charalampos Vasileiou, Panagiotis V. Iacovidou, Nicoletta Xanthos, Theodoros Biomed Res Int Research Article Aim. To evaluate the effects of erythropoietin administration on the adrenal glands in a swine model of ventricular fibrillation and resuscitation. Methods. Ventricular fibrillation was induced via pacing wire forwarded into the right ventricle in 20 female Landrace/Large White pigs, allocated into 2 groups: experimental group treated with bolus dose of erythropoietin (EPO) and control group which received normal saline. Cardiopulmonary resuscitation (CPR) was performed immediately after drug administration as per the 2010 European Resuscitation Council (ERC) guidelines for Advanced Life Support (ALS) until return of spontaneous circulation (ROSC) or death. Animals who achieved ROSC were monitored, mechanically ventilated, extubated, observed, and euthanized. At necroscopy, adrenal glands samples were formalin-fixed, paraffin-embedded, and routinely processed. Sections were stained with hematoxylin-eosin. Results. Oedema and apoptosis were the most frequent histological changes and were detected in all animals in the adrenal cortex and in the medulla. Mild and focal endothelial lesions were also detected. A marked interindividual variability in the degree of the intensity of apoptosis and oedema at cortical and medullary level was observed within groups. Comparing the two groups, higher levels of pathological changes were detected in the control group. No significant difference between the two groups was observed regarding the endothelial changes. Conclusions. In animals exposed to ventricular fibrillation, EPO treatment has protective effects on the adrenal gland. Hindawi Publishing Corporation 2016 2016-07-18 /pmc/articles/PMC4967690/ /pubmed/27504455 http://dx.doi.org/10.1155/2016/7261960 Text en Copyright © 2016 Armando Faa et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Faa, Armando
Faa, Gavino
Papalois, Apostolos
Obinu, Eleonora
Locci, Giorgia
Pais, Maria Elena
Lelovas, Pavlos
Barouxis, Dimitrios
Pantazopoulos, Charalampos
Vasileiou, Panagiotis V.
Iacovidou, Nicoletta
Xanthos, Theodoros
Effects of Erythropoietin Administration on Adrenal Glands of Landrace/Large White Pigs after Ventricular Fibrillation
title Effects of Erythropoietin Administration on Adrenal Glands of Landrace/Large White Pigs after Ventricular Fibrillation
title_full Effects of Erythropoietin Administration on Adrenal Glands of Landrace/Large White Pigs after Ventricular Fibrillation
title_fullStr Effects of Erythropoietin Administration on Adrenal Glands of Landrace/Large White Pigs after Ventricular Fibrillation
title_full_unstemmed Effects of Erythropoietin Administration on Adrenal Glands of Landrace/Large White Pigs after Ventricular Fibrillation
title_short Effects of Erythropoietin Administration on Adrenal Glands of Landrace/Large White Pigs after Ventricular Fibrillation
title_sort effects of erythropoietin administration on adrenal glands of landrace/large white pigs after ventricular fibrillation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4967690/
https://www.ncbi.nlm.nih.gov/pubmed/27504455
http://dx.doi.org/10.1155/2016/7261960
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