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Genome-wide Identification and Structural, Functional and Evolutionary Analysis of WRKY Components of Mulberry

Mulberry is known to be sensitive to several biotic and abiotic stresses, which in turn have a direct impact on the yield of silk, because it is the sole food source for the silk worm. WRKYs are a family of transcription factors, which play an important role in combating various biotic and abiotic s...

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Autores principales: Baranwal, Vinay Kumar, Negi, Nisha, Khurana, Paramjit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4967854/
https://www.ncbi.nlm.nih.gov/pubmed/27477686
http://dx.doi.org/10.1038/srep30794
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author Baranwal, Vinay Kumar
Negi, Nisha
Khurana, Paramjit
author_facet Baranwal, Vinay Kumar
Negi, Nisha
Khurana, Paramjit
author_sort Baranwal, Vinay Kumar
collection PubMed
description Mulberry is known to be sensitive to several biotic and abiotic stresses, which in turn have a direct impact on the yield of silk, because it is the sole food source for the silk worm. WRKYs are a family of transcription factors, which play an important role in combating various biotic and abiotic stresses. In this study, we identified 54 genes with conserved WRKY motifs in the Morus notabilis genome. Motif searches coupled with a phylogenetic analysis revealed seven sub-groups as well as the absence of members of Group Ib in mulberry. Analyses of the 2K upstream region in addition to a gene ontology terms enrichment analysis revealed putative functions of mulberry WRKYs under biotic and abiotic stresses. An RNA-seq-based analysis showed that several of the identified WRKYs have shown preferential expression in the leaf, bark, root, male flower, and winter bud of M. notabilis. Finally, expression analysis by qPCR under different stress and hormone treatments revealed genotype-specific responses. Taken together, our results briefs about the genome-wide identification of WRKYs as well as their differential response to stresses and hormones. Importantly, these data can also be utilized to identify potential molecular targets for conferring tolerance to various stresses in mulberry.
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spelling pubmed-49678542016-08-10 Genome-wide Identification and Structural, Functional and Evolutionary Analysis of WRKY Components of Mulberry Baranwal, Vinay Kumar Negi, Nisha Khurana, Paramjit Sci Rep Article Mulberry is known to be sensitive to several biotic and abiotic stresses, which in turn have a direct impact on the yield of silk, because it is the sole food source for the silk worm. WRKYs are a family of transcription factors, which play an important role in combating various biotic and abiotic stresses. In this study, we identified 54 genes with conserved WRKY motifs in the Morus notabilis genome. Motif searches coupled with a phylogenetic analysis revealed seven sub-groups as well as the absence of members of Group Ib in mulberry. Analyses of the 2K upstream region in addition to a gene ontology terms enrichment analysis revealed putative functions of mulberry WRKYs under biotic and abiotic stresses. An RNA-seq-based analysis showed that several of the identified WRKYs have shown preferential expression in the leaf, bark, root, male flower, and winter bud of M. notabilis. Finally, expression analysis by qPCR under different stress and hormone treatments revealed genotype-specific responses. Taken together, our results briefs about the genome-wide identification of WRKYs as well as their differential response to stresses and hormones. Importantly, these data can also be utilized to identify potential molecular targets for conferring tolerance to various stresses in mulberry. Nature Publishing Group 2016-08-01 /pmc/articles/PMC4967854/ /pubmed/27477686 http://dx.doi.org/10.1038/srep30794 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Baranwal, Vinay Kumar
Negi, Nisha
Khurana, Paramjit
Genome-wide Identification and Structural, Functional and Evolutionary Analysis of WRKY Components of Mulberry
title Genome-wide Identification and Structural, Functional and Evolutionary Analysis of WRKY Components of Mulberry
title_full Genome-wide Identification and Structural, Functional and Evolutionary Analysis of WRKY Components of Mulberry
title_fullStr Genome-wide Identification and Structural, Functional and Evolutionary Analysis of WRKY Components of Mulberry
title_full_unstemmed Genome-wide Identification and Structural, Functional and Evolutionary Analysis of WRKY Components of Mulberry
title_short Genome-wide Identification and Structural, Functional and Evolutionary Analysis of WRKY Components of Mulberry
title_sort genome-wide identification and structural, functional and evolutionary analysis of wrky components of mulberry
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4967854/
https://www.ncbi.nlm.nih.gov/pubmed/27477686
http://dx.doi.org/10.1038/srep30794
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