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Microbial Nanoculture as an Artificial Microniche
Microbes self-organize in microcolonies while transitioning to a sessile form within a protective biofilm matrix. To enable the detailed study of microbial dynamics within these microcolonies, new sessile culture systems are needed that sequester cells and mimic their complex growth conditions and i...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4967889/ https://www.ncbi.nlm.nih.gov/pubmed/27476816 http://dx.doi.org/10.1038/srep30578 |
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author | Niepa, Tagbo H. R. Hou, Likai Jiang, Hongyuan Goulian, Mark Koo, Hyun Stebe, Kathleen J. Lee, Daeyeon |
author_facet | Niepa, Tagbo H. R. Hou, Likai Jiang, Hongyuan Goulian, Mark Koo, Hyun Stebe, Kathleen J. Lee, Daeyeon |
author_sort | Niepa, Tagbo H. R. |
collection | PubMed |
description | Microbes self-organize in microcolonies while transitioning to a sessile form within a protective biofilm matrix. To enable the detailed study of microbial dynamics within these microcolonies, new sessile culture systems are needed that sequester cells and mimic their complex growth conditions and interactions. We present a new nanoliter-scale sessile culture system that is easily implemented via microfluidics-enabled fabrication. Hundreds of thousands of these nanocultures can be easily generated and imaged using conventional or confocal microscopy. Each nanoculture begins as a several nanoliter droplet of suspended cells, encapsulated by a polydimethylsiloxane (PDMS) membrane. The PDMS shell provides long-lasting mechanical support, enabling long term study, and is selectively permeable to small molecules including antibiotics, signaling molecules and functional fluorescent probes. Thus, as microcolonies mature within the nanocultures, they can be stressed or interrogated using selected probes to characterize cell physiological properties, antibiotic susceptibilities, and antagonistic interactions. We demonstrate this platform by investigating broad ranges of microcolony dynamics, including direct and indirect bacterial-fungal interactions. This versatile new tool has broad potential for addressing biological questions associated with drug resistance, chronic infections, microbiome dynamics, and antibiotic discovery. |
format | Online Article Text |
id | pubmed-4967889 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-49678892016-08-10 Microbial Nanoculture as an Artificial Microniche Niepa, Tagbo H. R. Hou, Likai Jiang, Hongyuan Goulian, Mark Koo, Hyun Stebe, Kathleen J. Lee, Daeyeon Sci Rep Article Microbes self-organize in microcolonies while transitioning to a sessile form within a protective biofilm matrix. To enable the detailed study of microbial dynamics within these microcolonies, new sessile culture systems are needed that sequester cells and mimic their complex growth conditions and interactions. We present a new nanoliter-scale sessile culture system that is easily implemented via microfluidics-enabled fabrication. Hundreds of thousands of these nanocultures can be easily generated and imaged using conventional or confocal microscopy. Each nanoculture begins as a several nanoliter droplet of suspended cells, encapsulated by a polydimethylsiloxane (PDMS) membrane. The PDMS shell provides long-lasting mechanical support, enabling long term study, and is selectively permeable to small molecules including antibiotics, signaling molecules and functional fluorescent probes. Thus, as microcolonies mature within the nanocultures, they can be stressed or interrogated using selected probes to characterize cell physiological properties, antibiotic susceptibilities, and antagonistic interactions. We demonstrate this platform by investigating broad ranges of microcolony dynamics, including direct and indirect bacterial-fungal interactions. This versatile new tool has broad potential for addressing biological questions associated with drug resistance, chronic infections, microbiome dynamics, and antibiotic discovery. Nature Publishing Group 2016-08-01 /pmc/articles/PMC4967889/ /pubmed/27476816 http://dx.doi.org/10.1038/srep30578 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Niepa, Tagbo H. R. Hou, Likai Jiang, Hongyuan Goulian, Mark Koo, Hyun Stebe, Kathleen J. Lee, Daeyeon Microbial Nanoculture as an Artificial Microniche |
title | Microbial Nanoculture as an Artificial Microniche |
title_full | Microbial Nanoculture as an Artificial Microniche |
title_fullStr | Microbial Nanoculture as an Artificial Microniche |
title_full_unstemmed | Microbial Nanoculture as an Artificial Microniche |
title_short | Microbial Nanoculture as an Artificial Microniche |
title_sort | microbial nanoculture as an artificial microniche |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4967889/ https://www.ncbi.nlm.nih.gov/pubmed/27476816 http://dx.doi.org/10.1038/srep30578 |
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