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Peptides interfering with protein-protein interactions in the ethylene signaling pathway delay tomato fruit ripening
The plant hormone ethylene is involved in the regulation of several processes with high importance for agricultural applications, e.g. ripening, aging and senescence. Previous work in our group has identified a small peptide (NOP-1) derived from the nuclear localization signal of the Arabidopsis eth...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4967898/ https://www.ncbi.nlm.nih.gov/pubmed/27477591 http://dx.doi.org/10.1038/srep30634 |
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author | Bisson, Melanie M. A. Kessenbrock, Mareike Müller, Lena Hofmann, Alexander Schmitz, Florian Cristescu, Simona M. Groth, Georg |
author_facet | Bisson, Melanie M. A. Kessenbrock, Mareike Müller, Lena Hofmann, Alexander Schmitz, Florian Cristescu, Simona M. Groth, Georg |
author_sort | Bisson, Melanie M. A. |
collection | PubMed |
description | The plant hormone ethylene is involved in the regulation of several processes with high importance for agricultural applications, e.g. ripening, aging and senescence. Previous work in our group has identified a small peptide (NOP-1) derived from the nuclear localization signal of the Arabidopsis ethylene regulator ETHYLENE INSENSITIVE-2 (EIN2) C-terminal part as efficient inhibitor of ethylene responses. Here, we show that NOP-1 is also able to efficiently disrupt EIN2-ETR1 complex formation in tomato, indicating that the NOP-1 inhibition mode is conserved across plant species. Surface application of NOP-1 on green tomato fruits delays ripening similar to known inhibitors of ethylene perception (MCP) and ethylene biosynthesis (AVG). Fruits treated with NOP-1 showed similar ethylene production as untreated controls underlining that NOP-1 blocks ethylene signaling by targeting an essential interaction in this pathway, while having no effect on ethylene biosynthesis. |
format | Online Article Text |
id | pubmed-4967898 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-49678982016-08-10 Peptides interfering with protein-protein interactions in the ethylene signaling pathway delay tomato fruit ripening Bisson, Melanie M. A. Kessenbrock, Mareike Müller, Lena Hofmann, Alexander Schmitz, Florian Cristescu, Simona M. Groth, Georg Sci Rep Article The plant hormone ethylene is involved in the regulation of several processes with high importance for agricultural applications, e.g. ripening, aging and senescence. Previous work in our group has identified a small peptide (NOP-1) derived from the nuclear localization signal of the Arabidopsis ethylene regulator ETHYLENE INSENSITIVE-2 (EIN2) C-terminal part as efficient inhibitor of ethylene responses. Here, we show that NOP-1 is also able to efficiently disrupt EIN2-ETR1 complex formation in tomato, indicating that the NOP-1 inhibition mode is conserved across plant species. Surface application of NOP-1 on green tomato fruits delays ripening similar to known inhibitors of ethylene perception (MCP) and ethylene biosynthesis (AVG). Fruits treated with NOP-1 showed similar ethylene production as untreated controls underlining that NOP-1 blocks ethylene signaling by targeting an essential interaction in this pathway, while having no effect on ethylene biosynthesis. Nature Publishing Group 2016-08-01 /pmc/articles/PMC4967898/ /pubmed/27477591 http://dx.doi.org/10.1038/srep30634 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Bisson, Melanie M. A. Kessenbrock, Mareike Müller, Lena Hofmann, Alexander Schmitz, Florian Cristescu, Simona M. Groth, Georg Peptides interfering with protein-protein interactions in the ethylene signaling pathway delay tomato fruit ripening |
title | Peptides interfering with protein-protein interactions in the ethylene signaling pathway delay tomato fruit ripening |
title_full | Peptides interfering with protein-protein interactions in the ethylene signaling pathway delay tomato fruit ripening |
title_fullStr | Peptides interfering with protein-protein interactions in the ethylene signaling pathway delay tomato fruit ripening |
title_full_unstemmed | Peptides interfering with protein-protein interactions in the ethylene signaling pathway delay tomato fruit ripening |
title_short | Peptides interfering with protein-protein interactions in the ethylene signaling pathway delay tomato fruit ripening |
title_sort | peptides interfering with protein-protein interactions in the ethylene signaling pathway delay tomato fruit ripening |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4967898/ https://www.ncbi.nlm.nih.gov/pubmed/27477591 http://dx.doi.org/10.1038/srep30634 |
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