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Peptides interfering with protein-protein interactions in the ethylene signaling pathway delay tomato fruit ripening

The plant hormone ethylene is involved in the regulation of several processes with high importance for agricultural applications, e.g. ripening, aging and senescence. Previous work in our group has identified a small peptide (NOP-1) derived from the nuclear localization signal of the Arabidopsis eth...

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Autores principales: Bisson, Melanie M. A., Kessenbrock, Mareike, Müller, Lena, Hofmann, Alexander, Schmitz, Florian, Cristescu, Simona M., Groth, Georg
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4967898/
https://www.ncbi.nlm.nih.gov/pubmed/27477591
http://dx.doi.org/10.1038/srep30634
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author Bisson, Melanie M. A.
Kessenbrock, Mareike
Müller, Lena
Hofmann, Alexander
Schmitz, Florian
Cristescu, Simona M.
Groth, Georg
author_facet Bisson, Melanie M. A.
Kessenbrock, Mareike
Müller, Lena
Hofmann, Alexander
Schmitz, Florian
Cristescu, Simona M.
Groth, Georg
author_sort Bisson, Melanie M. A.
collection PubMed
description The plant hormone ethylene is involved in the regulation of several processes with high importance for agricultural applications, e.g. ripening, aging and senescence. Previous work in our group has identified a small peptide (NOP-1) derived from the nuclear localization signal of the Arabidopsis ethylene regulator ETHYLENE INSENSITIVE-2 (EIN2) C-terminal part as efficient inhibitor of ethylene responses. Here, we show that NOP-1 is also able to efficiently disrupt EIN2-ETR1 complex formation in tomato, indicating that the NOP-1 inhibition mode is conserved across plant species. Surface application of NOP-1 on green tomato fruits delays ripening similar to known inhibitors of ethylene perception (MCP) and ethylene biosynthesis (AVG). Fruits treated with NOP-1 showed similar ethylene production as untreated controls underlining that NOP-1 blocks ethylene signaling by targeting an essential interaction in this pathway, while having no effect on ethylene biosynthesis.
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spelling pubmed-49678982016-08-10 Peptides interfering with protein-protein interactions in the ethylene signaling pathway delay tomato fruit ripening Bisson, Melanie M. A. Kessenbrock, Mareike Müller, Lena Hofmann, Alexander Schmitz, Florian Cristescu, Simona M. Groth, Georg Sci Rep Article The plant hormone ethylene is involved in the regulation of several processes with high importance for agricultural applications, e.g. ripening, aging and senescence. Previous work in our group has identified a small peptide (NOP-1) derived from the nuclear localization signal of the Arabidopsis ethylene regulator ETHYLENE INSENSITIVE-2 (EIN2) C-terminal part as efficient inhibitor of ethylene responses. Here, we show that NOP-1 is also able to efficiently disrupt EIN2-ETR1 complex formation in tomato, indicating that the NOP-1 inhibition mode is conserved across plant species. Surface application of NOP-1 on green tomato fruits delays ripening similar to known inhibitors of ethylene perception (MCP) and ethylene biosynthesis (AVG). Fruits treated with NOP-1 showed similar ethylene production as untreated controls underlining that NOP-1 blocks ethylene signaling by targeting an essential interaction in this pathway, while having no effect on ethylene biosynthesis. Nature Publishing Group 2016-08-01 /pmc/articles/PMC4967898/ /pubmed/27477591 http://dx.doi.org/10.1038/srep30634 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Bisson, Melanie M. A.
Kessenbrock, Mareike
Müller, Lena
Hofmann, Alexander
Schmitz, Florian
Cristescu, Simona M.
Groth, Georg
Peptides interfering with protein-protein interactions in the ethylene signaling pathway delay tomato fruit ripening
title Peptides interfering with protein-protein interactions in the ethylene signaling pathway delay tomato fruit ripening
title_full Peptides interfering with protein-protein interactions in the ethylene signaling pathway delay tomato fruit ripening
title_fullStr Peptides interfering with protein-protein interactions in the ethylene signaling pathway delay tomato fruit ripening
title_full_unstemmed Peptides interfering with protein-protein interactions in the ethylene signaling pathway delay tomato fruit ripening
title_short Peptides interfering with protein-protein interactions in the ethylene signaling pathway delay tomato fruit ripening
title_sort peptides interfering with protein-protein interactions in the ethylene signaling pathway delay tomato fruit ripening
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4967898/
https://www.ncbi.nlm.nih.gov/pubmed/27477591
http://dx.doi.org/10.1038/srep30634
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