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Single-shot diffraction data from the Mimivirus particle using an X-ray free-electron laser

Free-electron lasers (FEL) hold the potential to revolutionize structural biology by producing X-ray pules short enough to outrun radiation damage, thus allowing imaging of biological samples without the limitation from radiation damage. Thus, a major part of the scientific case for the first FELs w...

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Autores principales: Ekeberg, Tomas, Svenda, Martin, Seibert, M. Marvin, Abergel, Chantal, Maia, Filipe R.N.C., Seltzer, Virginie, DePonte, Daniel P., Aquila, Andrew, Andreasson, Jakob, Iwan, Bianca, Jönsson, Olof, Westphal, Daniel, Odić, Duško, Andersson, Inger, Barty, Anton, Liang, Meng, Martin, Andrew V., Gumprecht, Lars, Fleckenstein, Holger, Bajt, Saša, Barthelmess, Miriam, Coppola, Nicola, Claverie, Jean-Michel, Loh, N. Duane, Bostedt, Christoph, Bozek, John D., Krzywinski, Jacek, Messerschmidt, Marc, Bogan, Michael J., Hampton, Christina Y., Sierra, Raymond G., Frank, Matthias, Shoeman, Robert L., Lomb, Lukas, Foucar, Lutz, Epp, Sascha W., Rolles, Daniel, Rudenko, Artem, Hartmann, Robert, Hartmann, Andreas, Kimmel, Nils, Holl, Peter, Weidenspointner, Georg, Rudek, Benedikt, Erk, Benjamin, Kassemeyer, Stephan, Schlichting, Ilme, Strüder, Lothar, Ullrich, Joachim, Schmidt, Carlo, Krasniqi, Faton, Hauser, Günter, Reich, Christian, Soltau, Heike, Schorb, Sebastian, Hirsemann, Helmut, Wunderer, Cornelia, Graafsma, Heinz, Chapman, Henry, Hajdu, Janos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4968188/
https://www.ncbi.nlm.nih.gov/pubmed/27479754
http://dx.doi.org/10.1038/sdata.2016.60
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author Ekeberg, Tomas
Svenda, Martin
Seibert, M. Marvin
Abergel, Chantal
Maia, Filipe R.N.C.
Seltzer, Virginie
DePonte, Daniel P.
Aquila, Andrew
Andreasson, Jakob
Iwan, Bianca
Jönsson, Olof
Westphal, Daniel
Odić, Duško
Andersson, Inger
Barty, Anton
Liang, Meng
Martin, Andrew V.
Gumprecht, Lars
Fleckenstein, Holger
Bajt, Saša
Barthelmess, Miriam
Coppola, Nicola
Claverie, Jean-Michel
Loh, N. Duane
Bostedt, Christoph
Bozek, John D.
Krzywinski, Jacek
Messerschmidt, Marc
Bogan, Michael J.
Hampton, Christina Y.
Sierra, Raymond G.
Frank, Matthias
Shoeman, Robert L.
Lomb, Lukas
Foucar, Lutz
Epp, Sascha W.
Rolles, Daniel
Rudenko, Artem
Hartmann, Robert
Hartmann, Andreas
Kimmel, Nils
Holl, Peter
Weidenspointner, Georg
Rudek, Benedikt
Erk, Benjamin
Kassemeyer, Stephan
Schlichting, Ilme
Strüder, Lothar
Ullrich, Joachim
Schmidt, Carlo
Krasniqi, Faton
Hauser, Günter
Reich, Christian
Soltau, Heike
Schorb, Sebastian
Hirsemann, Helmut
Wunderer, Cornelia
Graafsma, Heinz
Chapman, Henry
Hajdu, Janos
author_facet Ekeberg, Tomas
Svenda, Martin
Seibert, M. Marvin
Abergel, Chantal
Maia, Filipe R.N.C.
Seltzer, Virginie
DePonte, Daniel P.
Aquila, Andrew
Andreasson, Jakob
Iwan, Bianca
Jönsson, Olof
Westphal, Daniel
Odić, Duško
Andersson, Inger
Barty, Anton
Liang, Meng
Martin, Andrew V.
Gumprecht, Lars
Fleckenstein, Holger
Bajt, Saša
Barthelmess, Miriam
Coppola, Nicola
Claverie, Jean-Michel
Loh, N. Duane
Bostedt, Christoph
Bozek, John D.
Krzywinski, Jacek
Messerschmidt, Marc
Bogan, Michael J.
Hampton, Christina Y.
Sierra, Raymond G.
Frank, Matthias
Shoeman, Robert L.
Lomb, Lukas
Foucar, Lutz
Epp, Sascha W.
Rolles, Daniel
Rudenko, Artem
Hartmann, Robert
Hartmann, Andreas
Kimmel, Nils
Holl, Peter
Weidenspointner, Georg
Rudek, Benedikt
Erk, Benjamin
Kassemeyer, Stephan
Schlichting, Ilme
Strüder, Lothar
Ullrich, Joachim
Schmidt, Carlo
Krasniqi, Faton
Hauser, Günter
Reich, Christian
Soltau, Heike
Schorb, Sebastian
Hirsemann, Helmut
Wunderer, Cornelia
Graafsma, Heinz
Chapman, Henry
Hajdu, Janos
author_sort Ekeberg, Tomas
collection PubMed
description Free-electron lasers (FEL) hold the potential to revolutionize structural biology by producing X-ray pules short enough to outrun radiation damage, thus allowing imaging of biological samples without the limitation from radiation damage. Thus, a major part of the scientific case for the first FELs was three-dimensional (3D) reconstruction of non-crystalline biological objects. In a recent publication we demonstrated the first 3D reconstruction of a biological object from an X-ray FEL using this technique. The sample was the giant Mimivirus, which is one of the largest known viruses with a diameter of 450 nm. Here we present the dataset used for this successful reconstruction. Data-analysis methods for single-particle imaging at FELs are undergoing heavy development but data collection relies on very limited time available through a highly competitive proposal process. This dataset provides experimental data to the entire community and could boost algorithm development and provide a benchmark dataset for new algorithms.
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spelling pubmed-49681882016-08-08 Single-shot diffraction data from the Mimivirus particle using an X-ray free-electron laser Ekeberg, Tomas Svenda, Martin Seibert, M. Marvin Abergel, Chantal Maia, Filipe R.N.C. Seltzer, Virginie DePonte, Daniel P. Aquila, Andrew Andreasson, Jakob Iwan, Bianca Jönsson, Olof Westphal, Daniel Odić, Duško Andersson, Inger Barty, Anton Liang, Meng Martin, Andrew V. Gumprecht, Lars Fleckenstein, Holger Bajt, Saša Barthelmess, Miriam Coppola, Nicola Claverie, Jean-Michel Loh, N. Duane Bostedt, Christoph Bozek, John D. Krzywinski, Jacek Messerschmidt, Marc Bogan, Michael J. Hampton, Christina Y. Sierra, Raymond G. Frank, Matthias Shoeman, Robert L. Lomb, Lukas Foucar, Lutz Epp, Sascha W. Rolles, Daniel Rudenko, Artem Hartmann, Robert Hartmann, Andreas Kimmel, Nils Holl, Peter Weidenspointner, Georg Rudek, Benedikt Erk, Benjamin Kassemeyer, Stephan Schlichting, Ilme Strüder, Lothar Ullrich, Joachim Schmidt, Carlo Krasniqi, Faton Hauser, Günter Reich, Christian Soltau, Heike Schorb, Sebastian Hirsemann, Helmut Wunderer, Cornelia Graafsma, Heinz Chapman, Henry Hajdu, Janos Sci Data Data Descriptor Free-electron lasers (FEL) hold the potential to revolutionize structural biology by producing X-ray pules short enough to outrun radiation damage, thus allowing imaging of biological samples without the limitation from radiation damage. Thus, a major part of the scientific case for the first FELs was three-dimensional (3D) reconstruction of non-crystalline biological objects. In a recent publication we demonstrated the first 3D reconstruction of a biological object from an X-ray FEL using this technique. The sample was the giant Mimivirus, which is one of the largest known viruses with a diameter of 450 nm. Here we present the dataset used for this successful reconstruction. Data-analysis methods for single-particle imaging at FELs are undergoing heavy development but data collection relies on very limited time available through a highly competitive proposal process. This dataset provides experimental data to the entire community and could boost algorithm development and provide a benchmark dataset for new algorithms. Nature Publishing Group 2016-08-01 /pmc/articles/PMC4968188/ /pubmed/27479754 http://dx.doi.org/10.1038/sdata.2016.60 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0 This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0 Metadata associated with this Data Descriptor is available at http://www.nature.com/sdata/ and is released under the CC0 waiver to maximize reuse.
spellingShingle Data Descriptor
Ekeberg, Tomas
Svenda, Martin
Seibert, M. Marvin
Abergel, Chantal
Maia, Filipe R.N.C.
Seltzer, Virginie
DePonte, Daniel P.
Aquila, Andrew
Andreasson, Jakob
Iwan, Bianca
Jönsson, Olof
Westphal, Daniel
Odić, Duško
Andersson, Inger
Barty, Anton
Liang, Meng
Martin, Andrew V.
Gumprecht, Lars
Fleckenstein, Holger
Bajt, Saša
Barthelmess, Miriam
Coppola, Nicola
Claverie, Jean-Michel
Loh, N. Duane
Bostedt, Christoph
Bozek, John D.
Krzywinski, Jacek
Messerschmidt, Marc
Bogan, Michael J.
Hampton, Christina Y.
Sierra, Raymond G.
Frank, Matthias
Shoeman, Robert L.
Lomb, Lukas
Foucar, Lutz
Epp, Sascha W.
Rolles, Daniel
Rudenko, Artem
Hartmann, Robert
Hartmann, Andreas
Kimmel, Nils
Holl, Peter
Weidenspointner, Georg
Rudek, Benedikt
Erk, Benjamin
Kassemeyer, Stephan
Schlichting, Ilme
Strüder, Lothar
Ullrich, Joachim
Schmidt, Carlo
Krasniqi, Faton
Hauser, Günter
Reich, Christian
Soltau, Heike
Schorb, Sebastian
Hirsemann, Helmut
Wunderer, Cornelia
Graafsma, Heinz
Chapman, Henry
Hajdu, Janos
Single-shot diffraction data from the Mimivirus particle using an X-ray free-electron laser
title Single-shot diffraction data from the Mimivirus particle using an X-ray free-electron laser
title_full Single-shot diffraction data from the Mimivirus particle using an X-ray free-electron laser
title_fullStr Single-shot diffraction data from the Mimivirus particle using an X-ray free-electron laser
title_full_unstemmed Single-shot diffraction data from the Mimivirus particle using an X-ray free-electron laser
title_short Single-shot diffraction data from the Mimivirus particle using an X-ray free-electron laser
title_sort single-shot diffraction data from the mimivirus particle using an x-ray free-electron laser
topic Data Descriptor
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4968188/
https://www.ncbi.nlm.nih.gov/pubmed/27479754
http://dx.doi.org/10.1038/sdata.2016.60
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