Cargando…

Fatty acid extract from CLA-enriched egg yolks can mediate transcriptome reprogramming of MCF-7 cancer cells to prevent their growth and proliferation

BACKGROUND: Our previous study showed that fatty acids extract obtained from CLA-enriched egg yolks (EFA-CLA) suppressed the viability of MCF-7 cancer cell line more effectively than extract from non-enriched egg yolks (EFA). In this study, we analysed the effect of EFA-CLA and EFA on transcriptome...

Descripción completa

Detalles Bibliográficos
Autores principales: Koronowicz, Aneta A., Banks, Paula, Domagała, Dominik, Master, Adam, Leszczyńska, Teresa, Piasna, Ewelina, Marynowska, Mariola, Laidler, Piotr
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4968440/
https://www.ncbi.nlm.nih.gov/pubmed/27551323
http://dx.doi.org/10.1186/s12263-016-0537-z
_version_ 1782445659745419264
author Koronowicz, Aneta A.
Banks, Paula
Domagała, Dominik
Master, Adam
Leszczyńska, Teresa
Piasna, Ewelina
Marynowska, Mariola
Laidler, Piotr
author_facet Koronowicz, Aneta A.
Banks, Paula
Domagała, Dominik
Master, Adam
Leszczyńska, Teresa
Piasna, Ewelina
Marynowska, Mariola
Laidler, Piotr
author_sort Koronowicz, Aneta A.
collection PubMed
description BACKGROUND: Our previous study showed that fatty acids extract obtained from CLA-enriched egg yolks (EFA-CLA) suppressed the viability of MCF-7 cancer cell line more effectively than extract from non-enriched egg yolks (EFA). In this study, we analysed the effect of EFA-CLA and EFA on transcriptome profile of MCF-7 cells by applying the whole Human Genome Microarray technology. RESULTS: We found that EFA-CLA and EFA treated cells differentially regulated genes involved in cancer development and progression. EFA-CLA, compared to EFA, positively increased the mRNA expression of TSC2 and PTEN tumor suppressors as well as decreased the expression of NOTCH1, AGPS, GNA12, STAT3, UCP2, HIGD2A, HIF1A, PPKAR1A oncogenes. CONCLUSIONS: We show for the first time that EFA-CLA can regulate genes engaged in AKT/mTOR pathway and inhibiting cell cycle progression. The observed results are most likely achieved by the combined effect of both: incorporated CLA isomers and other fatty acids in eggs organically modified through hens’ diet. Our results suggest that CLA-enriched eggs could be easily available food products with a potential of a cancer chemopreventive agent. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12263-016-0537-z) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-4968440
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-49684402016-08-22 Fatty acid extract from CLA-enriched egg yolks can mediate transcriptome reprogramming of MCF-7 cancer cells to prevent their growth and proliferation Koronowicz, Aneta A. Banks, Paula Domagała, Dominik Master, Adam Leszczyńska, Teresa Piasna, Ewelina Marynowska, Mariola Laidler, Piotr Genes Nutr Research BACKGROUND: Our previous study showed that fatty acids extract obtained from CLA-enriched egg yolks (EFA-CLA) suppressed the viability of MCF-7 cancer cell line more effectively than extract from non-enriched egg yolks (EFA). In this study, we analysed the effect of EFA-CLA and EFA on transcriptome profile of MCF-7 cells by applying the whole Human Genome Microarray technology. RESULTS: We found that EFA-CLA and EFA treated cells differentially regulated genes involved in cancer development and progression. EFA-CLA, compared to EFA, positively increased the mRNA expression of TSC2 and PTEN tumor suppressors as well as decreased the expression of NOTCH1, AGPS, GNA12, STAT3, UCP2, HIGD2A, HIF1A, PPKAR1A oncogenes. CONCLUSIONS: We show for the first time that EFA-CLA can regulate genes engaged in AKT/mTOR pathway and inhibiting cell cycle progression. The observed results are most likely achieved by the combined effect of both: incorporated CLA isomers and other fatty acids in eggs organically modified through hens’ diet. Our results suggest that CLA-enriched eggs could be easily available food products with a potential of a cancer chemopreventive agent. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12263-016-0537-z) contains supplementary material, which is available to authorized users. BioMed Central 2016-07-27 /pmc/articles/PMC4968440/ /pubmed/27551323 http://dx.doi.org/10.1186/s12263-016-0537-z Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Koronowicz, Aneta A.
Banks, Paula
Domagała, Dominik
Master, Adam
Leszczyńska, Teresa
Piasna, Ewelina
Marynowska, Mariola
Laidler, Piotr
Fatty acid extract from CLA-enriched egg yolks can mediate transcriptome reprogramming of MCF-7 cancer cells to prevent their growth and proliferation
title Fatty acid extract from CLA-enriched egg yolks can mediate transcriptome reprogramming of MCF-7 cancer cells to prevent their growth and proliferation
title_full Fatty acid extract from CLA-enriched egg yolks can mediate transcriptome reprogramming of MCF-7 cancer cells to prevent their growth and proliferation
title_fullStr Fatty acid extract from CLA-enriched egg yolks can mediate transcriptome reprogramming of MCF-7 cancer cells to prevent their growth and proliferation
title_full_unstemmed Fatty acid extract from CLA-enriched egg yolks can mediate transcriptome reprogramming of MCF-7 cancer cells to prevent their growth and proliferation
title_short Fatty acid extract from CLA-enriched egg yolks can mediate transcriptome reprogramming of MCF-7 cancer cells to prevent their growth and proliferation
title_sort fatty acid extract from cla-enriched egg yolks can mediate transcriptome reprogramming of mcf-7 cancer cells to prevent their growth and proliferation
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4968440/
https://www.ncbi.nlm.nih.gov/pubmed/27551323
http://dx.doi.org/10.1186/s12263-016-0537-z
work_keys_str_mv AT koronowiczanetaa fattyacidextractfromclaenrichedeggyolkscanmediatetranscriptomereprogrammingofmcf7cancercellstopreventtheirgrowthandproliferation
AT bankspaula fattyacidextractfromclaenrichedeggyolkscanmediatetranscriptomereprogrammingofmcf7cancercellstopreventtheirgrowthandproliferation
AT domagaładominik fattyacidextractfromclaenrichedeggyolkscanmediatetranscriptomereprogrammingofmcf7cancercellstopreventtheirgrowthandproliferation
AT masteradam fattyacidextractfromclaenrichedeggyolkscanmediatetranscriptomereprogrammingofmcf7cancercellstopreventtheirgrowthandproliferation
AT leszczynskateresa fattyacidextractfromclaenrichedeggyolkscanmediatetranscriptomereprogrammingofmcf7cancercellstopreventtheirgrowthandproliferation
AT piasnaewelina fattyacidextractfromclaenrichedeggyolkscanmediatetranscriptomereprogrammingofmcf7cancercellstopreventtheirgrowthandproliferation
AT marynowskamariola fattyacidextractfromclaenrichedeggyolkscanmediatetranscriptomereprogrammingofmcf7cancercellstopreventtheirgrowthandproliferation
AT laidlerpiotr fattyacidextractfromclaenrichedeggyolkscanmediatetranscriptomereprogrammingofmcf7cancercellstopreventtheirgrowthandproliferation