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Visible-Light Optical Coherence Tomography Angiography for Monitoring Laser-Induced Choroidal Neovascularization in Mice

PURPOSE: This study sought to determine the earliest time-point at which evidence of choroidal neovascularization (CNV) could be detected with visible-light optical coherence tomography angiography (vis-OCTA) in a mouse model of laser-induced CNV. METHODS: Visible light-OCTA was used to study laser-...

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Autores principales: Shah, Ronil S., Soetikno, Brian T., Yi, Ji, Liu, Wenzhong, Skondra, Dimitra, Zhang, Hao F., Fawzi, Amani A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Association for Research in Vision and Ophthalmology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4968775/
https://www.ncbi.nlm.nih.gov/pubmed/27409510
http://dx.doi.org/10.1167/iovs.15-18891
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author Shah, Ronil S.
Soetikno, Brian T.
Yi, Ji
Liu, Wenzhong
Skondra, Dimitra
Zhang, Hao F.
Fawzi, Amani A.
author_facet Shah, Ronil S.
Soetikno, Brian T.
Yi, Ji
Liu, Wenzhong
Skondra, Dimitra
Zhang, Hao F.
Fawzi, Amani A.
author_sort Shah, Ronil S.
collection PubMed
description PURPOSE: This study sought to determine the earliest time-point at which evidence of choroidal neovascularization (CNV) could be detected with visible-light optical coherence tomography angiography (vis-OCTA) in a mouse model of laser-induced CNV. METHODS: Visible light-OCTA was used to study laser-induced CNV at different time-points after laser injury to monitor CNV development and measure CNV lesion size. Measurements obtained from vis-OCTA angiograms were compared with histopathologic measurements from isolectin-stained choroidal flatmounts. RESULTS: Choroidal neovascularization area measurements between the vis-OCTA system and isolectin-stained choroidal flatmounts were significantly different in area for days 2 to 4 postlaser injury, and were not significantly different in area for days 5, 7, and 14. Choroidal neovascularization area measurements taken from the stained flatmounts were larger than their vis-OCTA counterparts for all time-points. Both modalities showed a similar trend of CNV size increasing from the day of laser injury until a peak of day 7 postlaser injury and subsequently decreasing by day 14. CONCLUSIONS: The earliest vis-OCTA can detect the presence of aberrant vessels in a mouse laser-induced CNV model is 5 days after laser injury. Visible light-OCTA was able to visualize the maximum of the CNV network 7 days postlaser injury, in accordance with choroidal flatmount immunostaining. Visible light-OCTA is a reliable tool in both detecting the presence of CNV development, as well as accurately determining the size of the lesion in a mouse laser-induced CNV model.
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spelling pubmed-49687752017-01-01 Visible-Light Optical Coherence Tomography Angiography for Monitoring Laser-Induced Choroidal Neovascularization in Mice Shah, Ronil S. Soetikno, Brian T. Yi, Ji Liu, Wenzhong Skondra, Dimitra Zhang, Hao F. Fawzi, Amani A. Invest Ophthalmol Vis Sci Articles PURPOSE: This study sought to determine the earliest time-point at which evidence of choroidal neovascularization (CNV) could be detected with visible-light optical coherence tomography angiography (vis-OCTA) in a mouse model of laser-induced CNV. METHODS: Visible light-OCTA was used to study laser-induced CNV at different time-points after laser injury to monitor CNV development and measure CNV lesion size. Measurements obtained from vis-OCTA angiograms were compared with histopathologic measurements from isolectin-stained choroidal flatmounts. RESULTS: Choroidal neovascularization area measurements between the vis-OCTA system and isolectin-stained choroidal flatmounts were significantly different in area for days 2 to 4 postlaser injury, and were not significantly different in area for days 5, 7, and 14. Choroidal neovascularization area measurements taken from the stained flatmounts were larger than their vis-OCTA counterparts for all time-points. Both modalities showed a similar trend of CNV size increasing from the day of laser injury until a peak of day 7 postlaser injury and subsequently decreasing by day 14. CONCLUSIONS: The earliest vis-OCTA can detect the presence of aberrant vessels in a mouse laser-induced CNV model is 5 days after laser injury. Visible light-OCTA was able to visualize the maximum of the CNV network 7 days postlaser injury, in accordance with choroidal flatmount immunostaining. Visible light-OCTA is a reliable tool in both detecting the presence of CNV development, as well as accurately determining the size of the lesion in a mouse laser-induced CNV model. The Association for Research in Vision and Ophthalmology 2016-07-13 2016-07 /pmc/articles/PMC4968775/ /pubmed/27409510 http://dx.doi.org/10.1167/iovs.15-18891 Text en http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
spellingShingle Articles
Shah, Ronil S.
Soetikno, Brian T.
Yi, Ji
Liu, Wenzhong
Skondra, Dimitra
Zhang, Hao F.
Fawzi, Amani A.
Visible-Light Optical Coherence Tomography Angiography for Monitoring Laser-Induced Choroidal Neovascularization in Mice
title Visible-Light Optical Coherence Tomography Angiography for Monitoring Laser-Induced Choroidal Neovascularization in Mice
title_full Visible-Light Optical Coherence Tomography Angiography for Monitoring Laser-Induced Choroidal Neovascularization in Mice
title_fullStr Visible-Light Optical Coherence Tomography Angiography for Monitoring Laser-Induced Choroidal Neovascularization in Mice
title_full_unstemmed Visible-Light Optical Coherence Tomography Angiography for Monitoring Laser-Induced Choroidal Neovascularization in Mice
title_short Visible-Light Optical Coherence Tomography Angiography for Monitoring Laser-Induced Choroidal Neovascularization in Mice
title_sort visible-light optical coherence tomography angiography for monitoring laser-induced choroidal neovascularization in mice
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4968775/
https://www.ncbi.nlm.nih.gov/pubmed/27409510
http://dx.doi.org/10.1167/iovs.15-18891
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