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Genome-Wide Association Study Reveals the Genetic Basis of Stalk Cell Wall Components in Maize
Lignin, cellulose and hemicellulose are the three main components of the plant cell wall and can impact stalk quality by affecting cell wall structure and strength. In this study, we evaluated the lignin (LIG), cellulose (CEL) and hemicellulose (HC) contents in maize using an association mapping pan...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4968789/ https://www.ncbi.nlm.nih.gov/pubmed/27479588 http://dx.doi.org/10.1371/journal.pone.0158906 |
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author | Li, Kun Wang, Hongwu Hu, Xiaojiao Liu, Zhifang Wu, Yujin Huang, Changling |
author_facet | Li, Kun Wang, Hongwu Hu, Xiaojiao Liu, Zhifang Wu, Yujin Huang, Changling |
author_sort | Li, Kun |
collection | PubMed |
description | Lignin, cellulose and hemicellulose are the three main components of the plant cell wall and can impact stalk quality by affecting cell wall structure and strength. In this study, we evaluated the lignin (LIG), cellulose (CEL) and hemicellulose (HC) contents in maize using an association mapping panel that included 368 inbred lines in seven environments. A genome-wide association study using approximately 0.56 million SNPs with a minor allele frequency of 0.05 identified 22, 18 and 24 loci significantly associated with LIG, CEL and HC at P < 1.0×10(−4), respectively. The allelic variation of each significant association contributed 4 to 7% of the phenotypic variation. Candidate genes identified by GWAS mainly encode enzymes involved in cell wall metabolism, transcription factors, protein kinase and protein related to other biological processes. Among the association signals, six candidate genes had pleiotropic effects on lignin and cellulose content. These results provide valuable information for better understanding the genetic basis of stalk cell wall components in maize. |
format | Online Article Text |
id | pubmed-4968789 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-49687892016-08-18 Genome-Wide Association Study Reveals the Genetic Basis of Stalk Cell Wall Components in Maize Li, Kun Wang, Hongwu Hu, Xiaojiao Liu, Zhifang Wu, Yujin Huang, Changling PLoS One Research Article Lignin, cellulose and hemicellulose are the three main components of the plant cell wall and can impact stalk quality by affecting cell wall structure and strength. In this study, we evaluated the lignin (LIG), cellulose (CEL) and hemicellulose (HC) contents in maize using an association mapping panel that included 368 inbred lines in seven environments. A genome-wide association study using approximately 0.56 million SNPs with a minor allele frequency of 0.05 identified 22, 18 and 24 loci significantly associated with LIG, CEL and HC at P < 1.0×10(−4), respectively. The allelic variation of each significant association contributed 4 to 7% of the phenotypic variation. Candidate genes identified by GWAS mainly encode enzymes involved in cell wall metabolism, transcription factors, protein kinase and protein related to other biological processes. Among the association signals, six candidate genes had pleiotropic effects on lignin and cellulose content. These results provide valuable information for better understanding the genetic basis of stalk cell wall components in maize. Public Library of Science 2016-08-01 /pmc/articles/PMC4968789/ /pubmed/27479588 http://dx.doi.org/10.1371/journal.pone.0158906 Text en © 2016 Li et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Li, Kun Wang, Hongwu Hu, Xiaojiao Liu, Zhifang Wu, Yujin Huang, Changling Genome-Wide Association Study Reveals the Genetic Basis of Stalk Cell Wall Components in Maize |
title | Genome-Wide Association Study Reveals the Genetic Basis of Stalk Cell Wall Components in Maize |
title_full | Genome-Wide Association Study Reveals the Genetic Basis of Stalk Cell Wall Components in Maize |
title_fullStr | Genome-Wide Association Study Reveals the Genetic Basis of Stalk Cell Wall Components in Maize |
title_full_unstemmed | Genome-Wide Association Study Reveals the Genetic Basis of Stalk Cell Wall Components in Maize |
title_short | Genome-Wide Association Study Reveals the Genetic Basis of Stalk Cell Wall Components in Maize |
title_sort | genome-wide association study reveals the genetic basis of stalk cell wall components in maize |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4968789/ https://www.ncbi.nlm.nih.gov/pubmed/27479588 http://dx.doi.org/10.1371/journal.pone.0158906 |
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