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Genome-Wide Association Study Reveals the Genetic Basis of Stalk Cell Wall Components in Maize

Lignin, cellulose and hemicellulose are the three main components of the plant cell wall and can impact stalk quality by affecting cell wall structure and strength. In this study, we evaluated the lignin (LIG), cellulose (CEL) and hemicellulose (HC) contents in maize using an association mapping pan...

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Detalles Bibliográficos
Autores principales: Li, Kun, Wang, Hongwu, Hu, Xiaojiao, Liu, Zhifang, Wu, Yujin, Huang, Changling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4968789/
https://www.ncbi.nlm.nih.gov/pubmed/27479588
http://dx.doi.org/10.1371/journal.pone.0158906
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author Li, Kun
Wang, Hongwu
Hu, Xiaojiao
Liu, Zhifang
Wu, Yujin
Huang, Changling
author_facet Li, Kun
Wang, Hongwu
Hu, Xiaojiao
Liu, Zhifang
Wu, Yujin
Huang, Changling
author_sort Li, Kun
collection PubMed
description Lignin, cellulose and hemicellulose are the three main components of the plant cell wall and can impact stalk quality by affecting cell wall structure and strength. In this study, we evaluated the lignin (LIG), cellulose (CEL) and hemicellulose (HC) contents in maize using an association mapping panel that included 368 inbred lines in seven environments. A genome-wide association study using approximately 0.56 million SNPs with a minor allele frequency of 0.05 identified 22, 18 and 24 loci significantly associated with LIG, CEL and HC at P < 1.0×10(−4), respectively. The allelic variation of each significant association contributed 4 to 7% of the phenotypic variation. Candidate genes identified by GWAS mainly encode enzymes involved in cell wall metabolism, transcription factors, protein kinase and protein related to other biological processes. Among the association signals, six candidate genes had pleiotropic effects on lignin and cellulose content. These results provide valuable information for better understanding the genetic basis of stalk cell wall components in maize.
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spelling pubmed-49687892016-08-18 Genome-Wide Association Study Reveals the Genetic Basis of Stalk Cell Wall Components in Maize Li, Kun Wang, Hongwu Hu, Xiaojiao Liu, Zhifang Wu, Yujin Huang, Changling PLoS One Research Article Lignin, cellulose and hemicellulose are the three main components of the plant cell wall and can impact stalk quality by affecting cell wall structure and strength. In this study, we evaluated the lignin (LIG), cellulose (CEL) and hemicellulose (HC) contents in maize using an association mapping panel that included 368 inbred lines in seven environments. A genome-wide association study using approximately 0.56 million SNPs with a minor allele frequency of 0.05 identified 22, 18 and 24 loci significantly associated with LIG, CEL and HC at P < 1.0×10(−4), respectively. The allelic variation of each significant association contributed 4 to 7% of the phenotypic variation. Candidate genes identified by GWAS mainly encode enzymes involved in cell wall metabolism, transcription factors, protein kinase and protein related to other biological processes. Among the association signals, six candidate genes had pleiotropic effects on lignin and cellulose content. These results provide valuable information for better understanding the genetic basis of stalk cell wall components in maize. Public Library of Science 2016-08-01 /pmc/articles/PMC4968789/ /pubmed/27479588 http://dx.doi.org/10.1371/journal.pone.0158906 Text en © 2016 Li et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Li, Kun
Wang, Hongwu
Hu, Xiaojiao
Liu, Zhifang
Wu, Yujin
Huang, Changling
Genome-Wide Association Study Reveals the Genetic Basis of Stalk Cell Wall Components in Maize
title Genome-Wide Association Study Reveals the Genetic Basis of Stalk Cell Wall Components in Maize
title_full Genome-Wide Association Study Reveals the Genetic Basis of Stalk Cell Wall Components in Maize
title_fullStr Genome-Wide Association Study Reveals the Genetic Basis of Stalk Cell Wall Components in Maize
title_full_unstemmed Genome-Wide Association Study Reveals the Genetic Basis of Stalk Cell Wall Components in Maize
title_short Genome-Wide Association Study Reveals the Genetic Basis of Stalk Cell Wall Components in Maize
title_sort genome-wide association study reveals the genetic basis of stalk cell wall components in maize
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4968789/
https://www.ncbi.nlm.nih.gov/pubmed/27479588
http://dx.doi.org/10.1371/journal.pone.0158906
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