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Sensitive and Quantitative Three-Color Protein Imaging in Fission Yeast Using Spectrally Diverse, Recoded Fluorescent Proteins with Experimentally-Characterized In Vivo Maturation Kinetics

Schizosaccharomyces pombe is an outstanding model organism for cell biological investigations, yet the range of useful and well-characterized fluorescent proteins (XFPs) is limited. We generated and characterized three recoded fluorescent proteins for 3-color analysis in S.pombe, Super-folder GFP, m...

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Detalles Bibliográficos
Autores principales: Al-Sady, Bassem, Greenstein, Rachel A., El-Samad, Hana J., Braun, Sigurd, Madhani, Hiten D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4968791/
https://www.ncbi.nlm.nih.gov/pubmed/27479698
http://dx.doi.org/10.1371/journal.pone.0159292