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Sensitive and Quantitative Three-Color Protein Imaging in Fission Yeast Using Spectrally Diverse, Recoded Fluorescent Proteins with Experimentally-Characterized In Vivo Maturation Kinetics
Schizosaccharomyces pombe is an outstanding model organism for cell biological investigations, yet the range of useful and well-characterized fluorescent proteins (XFPs) is limited. We generated and characterized three recoded fluorescent proteins for 3-color analysis in S.pombe, Super-folder GFP, m...
Autores principales: | Al-Sady, Bassem, Greenstein, Rachel A., El-Samad, Hana J., Braun, Sigurd, Madhani, Hiten D. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4968791/ https://www.ncbi.nlm.nih.gov/pubmed/27479698 http://dx.doi.org/10.1371/journal.pone.0159292 |
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