Cargando…

Genetic Diversity and Association Characters of Bacteria Isolated from Arbuscular Mycorrhizal Fungal Spore Walls

Association between arbuscular mycorrhizal fungi (AMF) and bacteria has long been studied. However, the factors influencing their association in the natural environment is still unknown. This study aimed to isolate bacteria associated with spore walls of AMF and identify their potential characters f...

Descripción completa

Detalles Bibliográficos
Autores principales: Selvakumar, Gopal, Krishnamoorthy, Ramasamy, Kim, Kiyoon, Sa, Tong-Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4968797/
https://www.ncbi.nlm.nih.gov/pubmed/27479250
http://dx.doi.org/10.1371/journal.pone.0160356
_version_ 1782445705612230656
author Selvakumar, Gopal
Krishnamoorthy, Ramasamy
Kim, Kiyoon
Sa, Tong-Min
author_facet Selvakumar, Gopal
Krishnamoorthy, Ramasamy
Kim, Kiyoon
Sa, Tong-Min
author_sort Selvakumar, Gopal
collection PubMed
description Association between arbuscular mycorrhizal fungi (AMF) and bacteria has long been studied. However, the factors influencing their association in the natural environment is still unknown. This study aimed to isolate bacteria associated with spore walls of AMF and identify their potential characters for association. Spores collected from coastal reclamation land were differentiated based on their morphology and identified by 18S rDNA sequencing as Funneliformis caledonium, Racocetra alborosea and Funneliformis mosseae. Bacteria associated with AMF spore walls were isolated after treating them with disinfection solution at different time intervals. After 0, 10 and 20 min of spore disinfection, 86, 24 and 10 spore associated bacteria (SAB) were isolated, respectively. BOX-PCR fingerprinting analysis showed that diverse bacterial communities were associated to AMF spores. Bacteria belonging to the same genera could associate with different AMF spores. Gram positive bacteria were more closely associated with AMF spores. Isolated SAB were characterized and tested for spore association characters such as chitinase, protease, cellulase enzymes and exopolysaccharide production (EPS). Among the 120 SAB, 113 SAB were able to show one or more characters for association and seven SAB did not show any association characters. The 16S rDNA sequence of SAB revealed that bacteria belonging to the phyla Firmicutes, Proteobacteria, Actinobacteria and Bactereiodes were associated with AMF spore walls.
format Online
Article
Text
id pubmed-4968797
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-49687972016-08-18 Genetic Diversity and Association Characters of Bacteria Isolated from Arbuscular Mycorrhizal Fungal Spore Walls Selvakumar, Gopal Krishnamoorthy, Ramasamy Kim, Kiyoon Sa, Tong-Min PLoS One Research Article Association between arbuscular mycorrhizal fungi (AMF) and bacteria has long been studied. However, the factors influencing their association in the natural environment is still unknown. This study aimed to isolate bacteria associated with spore walls of AMF and identify their potential characters for association. Spores collected from coastal reclamation land were differentiated based on their morphology and identified by 18S rDNA sequencing as Funneliformis caledonium, Racocetra alborosea and Funneliformis mosseae. Bacteria associated with AMF spore walls were isolated after treating them with disinfection solution at different time intervals. After 0, 10 and 20 min of spore disinfection, 86, 24 and 10 spore associated bacteria (SAB) were isolated, respectively. BOX-PCR fingerprinting analysis showed that diverse bacterial communities were associated to AMF spores. Bacteria belonging to the same genera could associate with different AMF spores. Gram positive bacteria were more closely associated with AMF spores. Isolated SAB were characterized and tested for spore association characters such as chitinase, protease, cellulase enzymes and exopolysaccharide production (EPS). Among the 120 SAB, 113 SAB were able to show one or more characters for association and seven SAB did not show any association characters. The 16S rDNA sequence of SAB revealed that bacteria belonging to the phyla Firmicutes, Proteobacteria, Actinobacteria and Bactereiodes were associated with AMF spore walls. Public Library of Science 2016-08-01 /pmc/articles/PMC4968797/ /pubmed/27479250 http://dx.doi.org/10.1371/journal.pone.0160356 Text en © 2016 Selvakumar et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Selvakumar, Gopal
Krishnamoorthy, Ramasamy
Kim, Kiyoon
Sa, Tong-Min
Genetic Diversity and Association Characters of Bacteria Isolated from Arbuscular Mycorrhizal Fungal Spore Walls
title Genetic Diversity and Association Characters of Bacteria Isolated from Arbuscular Mycorrhizal Fungal Spore Walls
title_full Genetic Diversity and Association Characters of Bacteria Isolated from Arbuscular Mycorrhizal Fungal Spore Walls
title_fullStr Genetic Diversity and Association Characters of Bacteria Isolated from Arbuscular Mycorrhizal Fungal Spore Walls
title_full_unstemmed Genetic Diversity and Association Characters of Bacteria Isolated from Arbuscular Mycorrhizal Fungal Spore Walls
title_short Genetic Diversity and Association Characters of Bacteria Isolated from Arbuscular Mycorrhizal Fungal Spore Walls
title_sort genetic diversity and association characters of bacteria isolated from arbuscular mycorrhizal fungal spore walls
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4968797/
https://www.ncbi.nlm.nih.gov/pubmed/27479250
http://dx.doi.org/10.1371/journal.pone.0160356
work_keys_str_mv AT selvakumargopal geneticdiversityandassociationcharactersofbacteriaisolatedfromarbuscularmycorrhizalfungalsporewalls
AT krishnamoorthyramasamy geneticdiversityandassociationcharactersofbacteriaisolatedfromarbuscularmycorrhizalfungalsporewalls
AT kimkiyoon geneticdiversityandassociationcharactersofbacteriaisolatedfromarbuscularmycorrhizalfungalsporewalls
AT satongmin geneticdiversityandassociationcharactersofbacteriaisolatedfromarbuscularmycorrhizalfungalsporewalls