Reorganisation of Hoxd regulatory landscapes during the evolution of a snake-like body plan
Within land vertebrate species, snakes display extreme variations in their body plan, characterized by the absence of limbs and an elongated morphology. Such a particular interpretation of the basic vertebrate body architecture has often been associated with changes in the function or regulation of...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4969037/ https://www.ncbi.nlm.nih.gov/pubmed/27476854 http://dx.doi.org/10.7554/eLife.16087 |
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author | Guerreiro, Isabel Gitto, Sandra Novoa, Ana Codourey, Julien Nguyen Huynh, Thi Hanh Gonzalez, Federico Milinkovitch, Michel C Mallo, Moises Duboule, Denis |
author_facet | Guerreiro, Isabel Gitto, Sandra Novoa, Ana Codourey, Julien Nguyen Huynh, Thi Hanh Gonzalez, Federico Milinkovitch, Michel C Mallo, Moises Duboule, Denis |
author_sort | Guerreiro, Isabel |
collection | PubMed |
description | Within land vertebrate species, snakes display extreme variations in their body plan, characterized by the absence of limbs and an elongated morphology. Such a particular interpretation of the basic vertebrate body architecture has often been associated with changes in the function or regulation of Hox genes. Here, we use an interspecies comparative approach to investigate different regulatory aspects at the snake HoxD locus. We report that, unlike in other vertebrates, snake mesoderm-specific enhancers are mostly located within the HoxD cluster itself rather than outside. In addition, despite both the absence of limbs and an altered Hoxd gene regulation in external genitalia, the limb-associated bimodal HoxD chromatin structure is maintained at the snake locus. Finally, we show that snake and mouse orthologous enhancer sequences can display distinct expression specificities. These results show that vertebrate morphological evolution likely involved extensive reorganisation at Hox loci, yet within a generally conserved regulatory framework. DOI: http://dx.doi.org/10.7554/eLife.16087.001 |
format | Online Article Text |
id | pubmed-4969037 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-49690372016-08-03 Reorganisation of Hoxd regulatory landscapes during the evolution of a snake-like body plan Guerreiro, Isabel Gitto, Sandra Novoa, Ana Codourey, Julien Nguyen Huynh, Thi Hanh Gonzalez, Federico Milinkovitch, Michel C Mallo, Moises Duboule, Denis eLife Developmental Biology and Stem Cells Within land vertebrate species, snakes display extreme variations in their body plan, characterized by the absence of limbs and an elongated morphology. Such a particular interpretation of the basic vertebrate body architecture has often been associated with changes in the function or regulation of Hox genes. Here, we use an interspecies comparative approach to investigate different regulatory aspects at the snake HoxD locus. We report that, unlike in other vertebrates, snake mesoderm-specific enhancers are mostly located within the HoxD cluster itself rather than outside. In addition, despite both the absence of limbs and an altered Hoxd gene regulation in external genitalia, the limb-associated bimodal HoxD chromatin structure is maintained at the snake locus. Finally, we show that snake and mouse orthologous enhancer sequences can display distinct expression specificities. These results show that vertebrate morphological evolution likely involved extensive reorganisation at Hox loci, yet within a generally conserved regulatory framework. DOI: http://dx.doi.org/10.7554/eLife.16087.001 eLife Sciences Publications, Ltd 2016-08-01 /pmc/articles/PMC4969037/ /pubmed/27476854 http://dx.doi.org/10.7554/eLife.16087 Text en © 2016, Guerreiro et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Developmental Biology and Stem Cells Guerreiro, Isabel Gitto, Sandra Novoa, Ana Codourey, Julien Nguyen Huynh, Thi Hanh Gonzalez, Federico Milinkovitch, Michel C Mallo, Moises Duboule, Denis Reorganisation of Hoxd regulatory landscapes during the evolution of a snake-like body plan |
title | Reorganisation of Hoxd regulatory landscapes during the evolution of a snake-like body plan |
title_full | Reorganisation of Hoxd regulatory landscapes during the evolution of a snake-like body plan |
title_fullStr | Reorganisation of Hoxd regulatory landscapes during the evolution of a snake-like body plan |
title_full_unstemmed | Reorganisation of Hoxd regulatory landscapes during the evolution of a snake-like body plan |
title_short | Reorganisation of Hoxd regulatory landscapes during the evolution of a snake-like body plan |
title_sort | reorganisation of hoxd regulatory landscapes during the evolution of a snake-like body plan |
topic | Developmental Biology and Stem Cells |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4969037/ https://www.ncbi.nlm.nih.gov/pubmed/27476854 http://dx.doi.org/10.7554/eLife.16087 |
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