Reorganisation of Hoxd regulatory landscapes during the evolution of a snake-like body plan

Within land vertebrate species, snakes display extreme variations in their body plan, characterized by the absence of limbs and an elongated morphology. Such a particular interpretation of the basic vertebrate body architecture has often been associated with changes in the function or regulation of...

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Autores principales: Guerreiro, Isabel, Gitto, Sandra, Novoa, Ana, Codourey, Julien, Nguyen Huynh, Thi Hanh, Gonzalez, Federico, Milinkovitch, Michel C, Mallo, Moises, Duboule, Denis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4969037/
https://www.ncbi.nlm.nih.gov/pubmed/27476854
http://dx.doi.org/10.7554/eLife.16087
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author Guerreiro, Isabel
Gitto, Sandra
Novoa, Ana
Codourey, Julien
Nguyen Huynh, Thi Hanh
Gonzalez, Federico
Milinkovitch, Michel C
Mallo, Moises
Duboule, Denis
author_facet Guerreiro, Isabel
Gitto, Sandra
Novoa, Ana
Codourey, Julien
Nguyen Huynh, Thi Hanh
Gonzalez, Federico
Milinkovitch, Michel C
Mallo, Moises
Duboule, Denis
author_sort Guerreiro, Isabel
collection PubMed
description Within land vertebrate species, snakes display extreme variations in their body plan, characterized by the absence of limbs and an elongated morphology. Such a particular interpretation of the basic vertebrate body architecture has often been associated with changes in the function or regulation of Hox genes. Here, we use an interspecies comparative approach to investigate different regulatory aspects at the snake HoxD locus. We report that, unlike in other vertebrates, snake mesoderm-specific enhancers are mostly located within the HoxD cluster itself rather than outside. In addition, despite both the absence of limbs and an altered Hoxd gene regulation in external genitalia, the limb-associated bimodal HoxD chromatin structure is maintained at the snake locus. Finally, we show that snake and mouse orthologous enhancer sequences can display distinct expression specificities. These results show that vertebrate morphological evolution likely involved extensive reorganisation at Hox loci, yet within a generally conserved regulatory framework. DOI: http://dx.doi.org/10.7554/eLife.16087.001
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spelling pubmed-49690372016-08-03 Reorganisation of Hoxd regulatory landscapes during the evolution of a snake-like body plan Guerreiro, Isabel Gitto, Sandra Novoa, Ana Codourey, Julien Nguyen Huynh, Thi Hanh Gonzalez, Federico Milinkovitch, Michel C Mallo, Moises Duboule, Denis eLife Developmental Biology and Stem Cells Within land vertebrate species, snakes display extreme variations in their body plan, characterized by the absence of limbs and an elongated morphology. Such a particular interpretation of the basic vertebrate body architecture has often been associated with changes in the function or regulation of Hox genes. Here, we use an interspecies comparative approach to investigate different regulatory aspects at the snake HoxD locus. We report that, unlike in other vertebrates, snake mesoderm-specific enhancers are mostly located within the HoxD cluster itself rather than outside. In addition, despite both the absence of limbs and an altered Hoxd gene regulation in external genitalia, the limb-associated bimodal HoxD chromatin structure is maintained at the snake locus. Finally, we show that snake and mouse orthologous enhancer sequences can display distinct expression specificities. These results show that vertebrate morphological evolution likely involved extensive reorganisation at Hox loci, yet within a generally conserved regulatory framework. DOI: http://dx.doi.org/10.7554/eLife.16087.001 eLife Sciences Publications, Ltd 2016-08-01 /pmc/articles/PMC4969037/ /pubmed/27476854 http://dx.doi.org/10.7554/eLife.16087 Text en © 2016, Guerreiro et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Developmental Biology and Stem Cells
Guerreiro, Isabel
Gitto, Sandra
Novoa, Ana
Codourey, Julien
Nguyen Huynh, Thi Hanh
Gonzalez, Federico
Milinkovitch, Michel C
Mallo, Moises
Duboule, Denis
Reorganisation of Hoxd regulatory landscapes during the evolution of a snake-like body plan
title Reorganisation of Hoxd regulatory landscapes during the evolution of a snake-like body plan
title_full Reorganisation of Hoxd regulatory landscapes during the evolution of a snake-like body plan
title_fullStr Reorganisation of Hoxd regulatory landscapes during the evolution of a snake-like body plan
title_full_unstemmed Reorganisation of Hoxd regulatory landscapes during the evolution of a snake-like body plan
title_short Reorganisation of Hoxd regulatory landscapes during the evolution of a snake-like body plan
title_sort reorganisation of hoxd regulatory landscapes during the evolution of a snake-like body plan
topic Developmental Biology and Stem Cells
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4969037/
https://www.ncbi.nlm.nih.gov/pubmed/27476854
http://dx.doi.org/10.7554/eLife.16087
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