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Radical scavenging and gastroprotective activity of methanolic extract of Gmelina arborea stem bark
BACKGROUND: Gmelina arborea (GA) is widely used in traditional medicine for treating a number of ailments including gastrointestinal tract disorders. OBJECTIVE: To evaluate the gastroprotective effect of GA stem bark against ethanol-induced gastric ulcer in Wistar rats. MATERIALS AND METHODS: All an...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4969311/ https://www.ncbi.nlm.nih.gov/pubmed/27449207 http://dx.doi.org/10.1016/j.jaim.2016.06.003 |
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author | Lawrence, Lincy Menon, Seema Vincent, Sheka Sivaram, Vipin P. Padikkala, Jose |
author_facet | Lawrence, Lincy Menon, Seema Vincent, Sheka Sivaram, Vipin P. Padikkala, Jose |
author_sort | Lawrence, Lincy |
collection | PubMed |
description | BACKGROUND: Gmelina arborea (GA) is widely used in traditional medicine for treating a number of ailments including gastrointestinal tract disorders. OBJECTIVE: To evaluate the gastroprotective effect of GA stem bark against ethanol-induced gastric ulcer in Wistar rats. MATERIALS AND METHODS: All animals were fasted for 36 h and received GA extract 250 and 500 mg/kg body weight (bw), 1 h before the administration of ethanol. The animals received ranitidine 50 mg/kg bw which served as the standard. The rats were sacrificed after 4 h. Then, the injuries to the gastric mucosa were estimated through gross evaluation of ulcer lesions and histology. The antioxidant parameters such as level of lipid peroxidation, superoxide dismutase (SOD), reduced glutathione (GSH), and glutathione peroxidase (GPx) in gastric tissue were also determined. RESULTS: GA treatment at a dose of 500 mg/kg bw offered 91.98% inhibition of ulcer formation, which is higher than that of ranitidine. The ethanol treatment extensively increased lipid peroxidation and it was significantly (P < 0.01) reduced in GA-treated group that eventually helped to prevent free radical accumulation. The GA enhanced the gastric mucosal antioxidant system, as indicated by a dose-dependent increase in the level/activities of GSH, GPx, and SOD. GA also attenuated the severity of histological signs of cell damage. Further, GA extract showed in-vitro 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity with IC(50) value of 124.39 μg/ml. CONCLUSION: The results indicate that the gastroprotective effect of GA is probably related to its antioxidant activities that protect gastric mucosa against oxidative damage and antilipid peroxidative activity that maintain membrane integrity. |
format | Online Article Text |
id | pubmed-4969311 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-49693112016-08-08 Radical scavenging and gastroprotective activity of methanolic extract of Gmelina arborea stem bark Lawrence, Lincy Menon, Seema Vincent, Sheka Sivaram, Vipin P. Padikkala, Jose J Ayurveda Integr Med Original Research Article (Experimental) BACKGROUND: Gmelina arborea (GA) is widely used in traditional medicine for treating a number of ailments including gastrointestinal tract disorders. OBJECTIVE: To evaluate the gastroprotective effect of GA stem bark against ethanol-induced gastric ulcer in Wistar rats. MATERIALS AND METHODS: All animals were fasted for 36 h and received GA extract 250 and 500 mg/kg body weight (bw), 1 h before the administration of ethanol. The animals received ranitidine 50 mg/kg bw which served as the standard. The rats were sacrificed after 4 h. Then, the injuries to the gastric mucosa were estimated through gross evaluation of ulcer lesions and histology. The antioxidant parameters such as level of lipid peroxidation, superoxide dismutase (SOD), reduced glutathione (GSH), and glutathione peroxidase (GPx) in gastric tissue were also determined. RESULTS: GA treatment at a dose of 500 mg/kg bw offered 91.98% inhibition of ulcer formation, which is higher than that of ranitidine. The ethanol treatment extensively increased lipid peroxidation and it was significantly (P < 0.01) reduced in GA-treated group that eventually helped to prevent free radical accumulation. The GA enhanced the gastric mucosal antioxidant system, as indicated by a dose-dependent increase in the level/activities of GSH, GPx, and SOD. GA also attenuated the severity of histological signs of cell damage. Further, GA extract showed in-vitro 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity with IC(50) value of 124.39 μg/ml. CONCLUSION: The results indicate that the gastroprotective effect of GA is probably related to its antioxidant activities that protect gastric mucosa against oxidative damage and antilipid peroxidative activity that maintain membrane integrity. Elsevier 2016 2016-07-20 /pmc/articles/PMC4969311/ /pubmed/27449207 http://dx.doi.org/10.1016/j.jaim.2016.06.003 Text en © 2016 Transdisciplinary University, Bangalore and World Ayurveda Foundation. Publishing Services by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Research Article (Experimental) Lawrence, Lincy Menon, Seema Vincent, Sheka Sivaram, Vipin P. Padikkala, Jose Radical scavenging and gastroprotective activity of methanolic extract of Gmelina arborea stem bark |
title | Radical scavenging and gastroprotective activity of methanolic extract of Gmelina arborea stem bark |
title_full | Radical scavenging and gastroprotective activity of methanolic extract of Gmelina arborea stem bark |
title_fullStr | Radical scavenging and gastroprotective activity of methanolic extract of Gmelina arborea stem bark |
title_full_unstemmed | Radical scavenging and gastroprotective activity of methanolic extract of Gmelina arborea stem bark |
title_short | Radical scavenging and gastroprotective activity of methanolic extract of Gmelina arborea stem bark |
title_sort | radical scavenging and gastroprotective activity of methanolic extract of gmelina arborea stem bark |
topic | Original Research Article (Experimental) |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4969311/ https://www.ncbi.nlm.nih.gov/pubmed/27449207 http://dx.doi.org/10.1016/j.jaim.2016.06.003 |
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