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Radical scavenging and gastroprotective activity of methanolic extract of Gmelina arborea stem bark

BACKGROUND: Gmelina arborea (GA) is widely used in traditional medicine for treating a number of ailments including gastrointestinal tract disorders. OBJECTIVE: To evaluate the gastroprotective effect of GA stem bark against ethanol-induced gastric ulcer in Wistar rats. MATERIALS AND METHODS: All an...

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Autores principales: Lawrence, Lincy, Menon, Seema, Vincent, Sheka, Sivaram, Vipin P., Padikkala, Jose
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4969311/
https://www.ncbi.nlm.nih.gov/pubmed/27449207
http://dx.doi.org/10.1016/j.jaim.2016.06.003
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author Lawrence, Lincy
Menon, Seema
Vincent, Sheka
Sivaram, Vipin P.
Padikkala, Jose
author_facet Lawrence, Lincy
Menon, Seema
Vincent, Sheka
Sivaram, Vipin P.
Padikkala, Jose
author_sort Lawrence, Lincy
collection PubMed
description BACKGROUND: Gmelina arborea (GA) is widely used in traditional medicine for treating a number of ailments including gastrointestinal tract disorders. OBJECTIVE: To evaluate the gastroprotective effect of GA stem bark against ethanol-induced gastric ulcer in Wistar rats. MATERIALS AND METHODS: All animals were fasted for 36 h and received GA extract 250 and 500 mg/kg body weight (bw), 1 h before the administration of ethanol. The animals received ranitidine 50 mg/kg bw which served as the standard. The rats were sacrificed after 4 h. Then, the injuries to the gastric mucosa were estimated through gross evaluation of ulcer lesions and histology. The antioxidant parameters such as level of lipid peroxidation, superoxide dismutase (SOD), reduced glutathione (GSH), and glutathione peroxidase (GPx) in gastric tissue were also determined. RESULTS: GA treatment at a dose of 500 mg/kg bw offered 91.98% inhibition of ulcer formation, which is higher than that of ranitidine. The ethanol treatment extensively increased lipid peroxidation and it was significantly (P < 0.01) reduced in GA-treated group that eventually helped to prevent free radical accumulation. The GA enhanced the gastric mucosal antioxidant system, as indicated by a dose-dependent increase in the level/activities of GSH, GPx, and SOD. GA also attenuated the severity of histological signs of cell damage. Further, GA extract showed in-vitro 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity with IC(50) value of 124.39 μg/ml. CONCLUSION: The results indicate that the gastroprotective effect of GA is probably related to its antioxidant activities that protect gastric mucosa against oxidative damage and antilipid peroxidative activity that maintain membrane integrity.
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spelling pubmed-49693112016-08-08 Radical scavenging and gastroprotective activity of methanolic extract of Gmelina arborea stem bark Lawrence, Lincy Menon, Seema Vincent, Sheka Sivaram, Vipin P. Padikkala, Jose J Ayurveda Integr Med Original Research Article (Experimental) BACKGROUND: Gmelina arborea (GA) is widely used in traditional medicine for treating a number of ailments including gastrointestinal tract disorders. OBJECTIVE: To evaluate the gastroprotective effect of GA stem bark against ethanol-induced gastric ulcer in Wistar rats. MATERIALS AND METHODS: All animals were fasted for 36 h and received GA extract 250 and 500 mg/kg body weight (bw), 1 h before the administration of ethanol. The animals received ranitidine 50 mg/kg bw which served as the standard. The rats were sacrificed after 4 h. Then, the injuries to the gastric mucosa were estimated through gross evaluation of ulcer lesions and histology. The antioxidant parameters such as level of lipid peroxidation, superoxide dismutase (SOD), reduced glutathione (GSH), and glutathione peroxidase (GPx) in gastric tissue were also determined. RESULTS: GA treatment at a dose of 500 mg/kg bw offered 91.98% inhibition of ulcer formation, which is higher than that of ranitidine. The ethanol treatment extensively increased lipid peroxidation and it was significantly (P < 0.01) reduced in GA-treated group that eventually helped to prevent free radical accumulation. The GA enhanced the gastric mucosal antioxidant system, as indicated by a dose-dependent increase in the level/activities of GSH, GPx, and SOD. GA also attenuated the severity of histological signs of cell damage. Further, GA extract showed in-vitro 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity with IC(50) value of 124.39 μg/ml. CONCLUSION: The results indicate that the gastroprotective effect of GA is probably related to its antioxidant activities that protect gastric mucosa against oxidative damage and antilipid peroxidative activity that maintain membrane integrity. Elsevier 2016 2016-07-20 /pmc/articles/PMC4969311/ /pubmed/27449207 http://dx.doi.org/10.1016/j.jaim.2016.06.003 Text en © 2016 Transdisciplinary University, Bangalore and World Ayurveda Foundation. Publishing Services by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Research Article (Experimental)
Lawrence, Lincy
Menon, Seema
Vincent, Sheka
Sivaram, Vipin P.
Padikkala, Jose
Radical scavenging and gastroprotective activity of methanolic extract of Gmelina arborea stem bark
title Radical scavenging and gastroprotective activity of methanolic extract of Gmelina arborea stem bark
title_full Radical scavenging and gastroprotective activity of methanolic extract of Gmelina arborea stem bark
title_fullStr Radical scavenging and gastroprotective activity of methanolic extract of Gmelina arborea stem bark
title_full_unstemmed Radical scavenging and gastroprotective activity of methanolic extract of Gmelina arborea stem bark
title_short Radical scavenging and gastroprotective activity of methanolic extract of Gmelina arborea stem bark
title_sort radical scavenging and gastroprotective activity of methanolic extract of gmelina arborea stem bark
topic Original Research Article (Experimental)
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4969311/
https://www.ncbi.nlm.nih.gov/pubmed/27449207
http://dx.doi.org/10.1016/j.jaim.2016.06.003
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