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Histone acetyl transferase GCN5 promotes human hepatocellular carcinoma progression by enhancing AIB1 expression

BACKGROUND: General control non-depressible 5 (GCN5) is a crucial catalytic component of a transcriptional regulatory complex that plays important roles in cellular functions from cell cycle regulation to DNA damage repair. Although GCN5 has recently been implicated in certain oncogenic roles, its r...

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Autores principales: Majaz, Sidra, Tong, Zhangwei, Peng, Kesong, Wang, Wei, Ren, Wenjing, Li, Ming, Liu, Kun, Mo, Pingli, Li, Wengang, Yu, Chundong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4969657/
https://www.ncbi.nlm.nih.gov/pubmed/27486509
http://dx.doi.org/10.1186/s13578-016-0114-6
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author Majaz, Sidra
Tong, Zhangwei
Peng, Kesong
Wang, Wei
Ren, Wenjing
Li, Ming
Liu, Kun
Mo, Pingli
Li, Wengang
Yu, Chundong
author_facet Majaz, Sidra
Tong, Zhangwei
Peng, Kesong
Wang, Wei
Ren, Wenjing
Li, Ming
Liu, Kun
Mo, Pingli
Li, Wengang
Yu, Chundong
author_sort Majaz, Sidra
collection PubMed
description BACKGROUND: General control non-depressible 5 (GCN5) is a crucial catalytic component of a transcriptional regulatory complex that plays important roles in cellular functions from cell cycle regulation to DNA damage repair. Although GCN5 has recently been implicated in certain oncogenic roles, its role in liver cancer progression remains vague. RESULTS: In this study, we report that GCN5 was overexpressed in 17 (54.8 %) of 31 human hepatocellular carcinoma (HCC) specimens. Down-regulation of GCN5 inhibited HCC cell proliferation and xenograft tumor formation. GCN5 knockdown decreased the protein levels of the proliferation marker proliferating cell nuclear antigen (PCNA) and amplified in breast cancer 1 (AIB1), but increased the protein levels of cell cycle inhibitor p21(Cip1/Waf1) in HepG2 cells. GCN5 regulated AIB1 expression, at least in part, by cooperating with E2F1 to enhance AIB1 transcription. Consistently, GCN5 expression was positively correlated with AIB1 expression in human HCC specimens in two GEO profile datasets. CONCLUSION: Since AIB1 plays a promoting role in HCC progression, our results propose that GCN5 promotes HCC progression at least partially by regulating AIB1 expression. This study implicates that GCN5 might be a potential molecular target for HCC diagnosis and treatment.
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spelling pubmed-49696572016-08-03 Histone acetyl transferase GCN5 promotes human hepatocellular carcinoma progression by enhancing AIB1 expression Majaz, Sidra Tong, Zhangwei Peng, Kesong Wang, Wei Ren, Wenjing Li, Ming Liu, Kun Mo, Pingli Li, Wengang Yu, Chundong Cell Biosci Research BACKGROUND: General control non-depressible 5 (GCN5) is a crucial catalytic component of a transcriptional regulatory complex that plays important roles in cellular functions from cell cycle regulation to DNA damage repair. Although GCN5 has recently been implicated in certain oncogenic roles, its role in liver cancer progression remains vague. RESULTS: In this study, we report that GCN5 was overexpressed in 17 (54.8 %) of 31 human hepatocellular carcinoma (HCC) specimens. Down-regulation of GCN5 inhibited HCC cell proliferation and xenograft tumor formation. GCN5 knockdown decreased the protein levels of the proliferation marker proliferating cell nuclear antigen (PCNA) and amplified in breast cancer 1 (AIB1), but increased the protein levels of cell cycle inhibitor p21(Cip1/Waf1) in HepG2 cells. GCN5 regulated AIB1 expression, at least in part, by cooperating with E2F1 to enhance AIB1 transcription. Consistently, GCN5 expression was positively correlated with AIB1 expression in human HCC specimens in two GEO profile datasets. CONCLUSION: Since AIB1 plays a promoting role in HCC progression, our results propose that GCN5 promotes HCC progression at least partially by regulating AIB1 expression. This study implicates that GCN5 might be a potential molecular target for HCC diagnosis and treatment. BioMed Central 2016-08-02 /pmc/articles/PMC4969657/ /pubmed/27486509 http://dx.doi.org/10.1186/s13578-016-0114-6 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Majaz, Sidra
Tong, Zhangwei
Peng, Kesong
Wang, Wei
Ren, Wenjing
Li, Ming
Liu, Kun
Mo, Pingli
Li, Wengang
Yu, Chundong
Histone acetyl transferase GCN5 promotes human hepatocellular carcinoma progression by enhancing AIB1 expression
title Histone acetyl transferase GCN5 promotes human hepatocellular carcinoma progression by enhancing AIB1 expression
title_full Histone acetyl transferase GCN5 promotes human hepatocellular carcinoma progression by enhancing AIB1 expression
title_fullStr Histone acetyl transferase GCN5 promotes human hepatocellular carcinoma progression by enhancing AIB1 expression
title_full_unstemmed Histone acetyl transferase GCN5 promotes human hepatocellular carcinoma progression by enhancing AIB1 expression
title_short Histone acetyl transferase GCN5 promotes human hepatocellular carcinoma progression by enhancing AIB1 expression
title_sort histone acetyl transferase gcn5 promotes human hepatocellular carcinoma progression by enhancing aib1 expression
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4969657/
https://www.ncbi.nlm.nih.gov/pubmed/27486509
http://dx.doi.org/10.1186/s13578-016-0114-6
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