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Role of the Gut Microbiome in Modulating Arthritis Progression in Mice
Genetics alone cannot explain most cases of rheumatoid arthritis (RA). Thus, investigating environmental factors such as the gut microbiota may provide new insights into the initiation and progression of RA. In this study, we performed 16S rRNA sequencing to characterise the gut microbiota of DBA1 m...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4969881/ https://www.ncbi.nlm.nih.gov/pubmed/27481047 http://dx.doi.org/10.1038/srep30594 |
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author | Liu, Xiaofei Zeng, Benhua Zhang, Juan Li, Wenxia Mou, Fangxiang Wang, Heng Zou, Qinghua Zhong, Bing Wu, Like Wei, Hong Fang, Yongfei |
author_facet | Liu, Xiaofei Zeng, Benhua Zhang, Juan Li, Wenxia Mou, Fangxiang Wang, Heng Zou, Qinghua Zhong, Bing Wu, Like Wei, Hong Fang, Yongfei |
author_sort | Liu, Xiaofei |
collection | PubMed |
description | Genetics alone cannot explain most cases of rheumatoid arthritis (RA). Thus, investigating environmental factors such as the gut microbiota may provide new insights into the initiation and progression of RA. In this study, we performed 16S rRNA sequencing to characterise the gut microbiota of DBA1 mice that did or did not develop arthritis after induction with collagen. We found that divergence in the distribution of microbiota after induction was pronounced and significant. Mice susceptible to collagen-induced arthritis (CIA) showed enriched operational taxonomic units (OTUs) affiliated with the genus Lactobacillus as the dominant genus prior to arthritis onset. With disease development, the abundance of OTUs affiliated with the families Bacteroidaceae, Lachnospiraceae, and S24-7 increased significantly in CIA-susceptible mice. Notably, germ-free mice conventionalized with the microbiota from CIA-susceptible mice showed a higher frequency of arthritis induction than those conventionalized with the microbiota from CIA-resistant mice. Consistently, the concentration of the cytokine interleukin-17 in serum and the proportions of CD8+T cells and Th17 lymphocytes in the spleen were significantly higher in the former group, whereas the abundances of dendritic cells, B cells, and Treg cells in the spleen were significantly lower. Our results suggest that the gut microbiome influences arthritis susceptibility. |
format | Online Article Text |
id | pubmed-4969881 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-49698812016-08-11 Role of the Gut Microbiome in Modulating Arthritis Progression in Mice Liu, Xiaofei Zeng, Benhua Zhang, Juan Li, Wenxia Mou, Fangxiang Wang, Heng Zou, Qinghua Zhong, Bing Wu, Like Wei, Hong Fang, Yongfei Sci Rep Article Genetics alone cannot explain most cases of rheumatoid arthritis (RA). Thus, investigating environmental factors such as the gut microbiota may provide new insights into the initiation and progression of RA. In this study, we performed 16S rRNA sequencing to characterise the gut microbiota of DBA1 mice that did or did not develop arthritis after induction with collagen. We found that divergence in the distribution of microbiota after induction was pronounced and significant. Mice susceptible to collagen-induced arthritis (CIA) showed enriched operational taxonomic units (OTUs) affiliated with the genus Lactobacillus as the dominant genus prior to arthritis onset. With disease development, the abundance of OTUs affiliated with the families Bacteroidaceae, Lachnospiraceae, and S24-7 increased significantly in CIA-susceptible mice. Notably, germ-free mice conventionalized with the microbiota from CIA-susceptible mice showed a higher frequency of arthritis induction than those conventionalized with the microbiota from CIA-resistant mice. Consistently, the concentration of the cytokine interleukin-17 in serum and the proportions of CD8+T cells and Th17 lymphocytes in the spleen were significantly higher in the former group, whereas the abundances of dendritic cells, B cells, and Treg cells in the spleen were significantly lower. Our results suggest that the gut microbiome influences arthritis susceptibility. Nature Publishing Group 2016-08-02 /pmc/articles/PMC4969881/ /pubmed/27481047 http://dx.doi.org/10.1038/srep30594 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Liu, Xiaofei Zeng, Benhua Zhang, Juan Li, Wenxia Mou, Fangxiang Wang, Heng Zou, Qinghua Zhong, Bing Wu, Like Wei, Hong Fang, Yongfei Role of the Gut Microbiome in Modulating Arthritis Progression in Mice |
title | Role of the Gut Microbiome in Modulating Arthritis Progression in Mice |
title_full | Role of the Gut Microbiome in Modulating Arthritis Progression in Mice |
title_fullStr | Role of the Gut Microbiome in Modulating Arthritis Progression in Mice |
title_full_unstemmed | Role of the Gut Microbiome in Modulating Arthritis Progression in Mice |
title_short | Role of the Gut Microbiome in Modulating Arthritis Progression in Mice |
title_sort | role of the gut microbiome in modulating arthritis progression in mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4969881/ https://www.ncbi.nlm.nih.gov/pubmed/27481047 http://dx.doi.org/10.1038/srep30594 |
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