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Effect of High-Fat Diet on the Formation of Pulmonary Neutrophil Extracellular Traps during Influenza Pneumonia in BALB/c Mice

Obesity is an independent risk factor for severe outcome of influenza infection. Higher dietary fat consumption has been linked to greater morbidity and severe influenza in mouse models. However, the extent of generation of neutrophil extracellular traps (NETs or NETosis) in obese individuals during...

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Autores principales: Moorthy, Anandi Narayana, Tan, Kong Bing, Wang, Shi, Narasaraju, Teluguakula, Chow, Vincent T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4969943/
https://www.ncbi.nlm.nih.gov/pubmed/27531997
http://dx.doi.org/10.3389/fimmu.2016.00289
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author Moorthy, Anandi Narayana
Tan, Kong Bing
Wang, Shi
Narasaraju, Teluguakula
Chow, Vincent T.
author_facet Moorthy, Anandi Narayana
Tan, Kong Bing
Wang, Shi
Narasaraju, Teluguakula
Chow, Vincent T.
author_sort Moorthy, Anandi Narayana
collection PubMed
description Obesity is an independent risk factor for severe outcome of influenza infection. Higher dietary fat consumption has been linked to greater morbidity and severe influenza in mouse models. However, the extent of generation of neutrophil extracellular traps (NETs or NETosis) in obese individuals during influenza pneumonia is hitherto unknown. This study investigated pulmonary NETs generation in BALB/c mice fed with high-fat diet (HFD) and low-fat diet (LFD), during the course of influenza pneumonia. Clinical disease progression, histopathology, lung reactive oxygen species, and myeloperoxidase activity were also compared. Consumption of HFD over 18 weeks led to significantly higher body weight, body mass index, and adiposity in BALB/c mice compared with LFD. Lethal challenge of mice (on HFD and LFD) with influenza A/PR/8/34 (H1N1) virus led to similar body weight loss and histopathologic severity. However, NETs were formed at relatively higher levels in mice fed with HFD, despite the absence of significant difference in disease progression between HFD- and LFD-fed mice.
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spelling pubmed-49699432016-08-16 Effect of High-Fat Diet on the Formation of Pulmonary Neutrophil Extracellular Traps during Influenza Pneumonia in BALB/c Mice Moorthy, Anandi Narayana Tan, Kong Bing Wang, Shi Narasaraju, Teluguakula Chow, Vincent T. Front Immunol Immunology Obesity is an independent risk factor for severe outcome of influenza infection. Higher dietary fat consumption has been linked to greater morbidity and severe influenza in mouse models. However, the extent of generation of neutrophil extracellular traps (NETs or NETosis) in obese individuals during influenza pneumonia is hitherto unknown. This study investigated pulmonary NETs generation in BALB/c mice fed with high-fat diet (HFD) and low-fat diet (LFD), during the course of influenza pneumonia. Clinical disease progression, histopathology, lung reactive oxygen species, and myeloperoxidase activity were also compared. Consumption of HFD over 18 weeks led to significantly higher body weight, body mass index, and adiposity in BALB/c mice compared with LFD. Lethal challenge of mice (on HFD and LFD) with influenza A/PR/8/34 (H1N1) virus led to similar body weight loss and histopathologic severity. However, NETs were formed at relatively higher levels in mice fed with HFD, despite the absence of significant difference in disease progression between HFD- and LFD-fed mice. Frontiers Media S.A. 2016-08-02 /pmc/articles/PMC4969943/ /pubmed/27531997 http://dx.doi.org/10.3389/fimmu.2016.00289 Text en Copyright © 2016 Moorthy, Tan, Wang, Narasaraju and Chow. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Moorthy, Anandi Narayana
Tan, Kong Bing
Wang, Shi
Narasaraju, Teluguakula
Chow, Vincent T.
Effect of High-Fat Diet on the Formation of Pulmonary Neutrophil Extracellular Traps during Influenza Pneumonia in BALB/c Mice
title Effect of High-Fat Diet on the Formation of Pulmonary Neutrophil Extracellular Traps during Influenza Pneumonia in BALB/c Mice
title_full Effect of High-Fat Diet on the Formation of Pulmonary Neutrophil Extracellular Traps during Influenza Pneumonia in BALB/c Mice
title_fullStr Effect of High-Fat Diet on the Formation of Pulmonary Neutrophil Extracellular Traps during Influenza Pneumonia in BALB/c Mice
title_full_unstemmed Effect of High-Fat Diet on the Formation of Pulmonary Neutrophil Extracellular Traps during Influenza Pneumonia in BALB/c Mice
title_short Effect of High-Fat Diet on the Formation of Pulmonary Neutrophil Extracellular Traps during Influenza Pneumonia in BALB/c Mice
title_sort effect of high-fat diet on the formation of pulmonary neutrophil extracellular traps during influenza pneumonia in balb/c mice
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4969943/
https://www.ncbi.nlm.nih.gov/pubmed/27531997
http://dx.doi.org/10.3389/fimmu.2016.00289
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