Cargando…

Single-Use Disposable Electrochemical Label-Free Immunosensor for Detection of Glycated Hemoglobin (HbA1c) Using Differential Pulse Voltammetry (DPV)

A single-use disposable in vitro electrochemical immunosensor for the detection of HbA1c in undiluted human serum using differential pulse voltammetry (DPV) was developed. A three-electrode configuration electrochemical biosensor consisted of 10-nm-thin gold film working and counter electrodes and a...

Descripción completa

Detalles Bibliográficos
Autores principales: Molazemhosseini, Alireza, Magagnin, Luca, Vena, Pasquale, Liu, Chung-Chiun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4970074/
https://www.ncbi.nlm.nih.gov/pubmed/27376299
http://dx.doi.org/10.3390/s16071024
_version_ 1782445906076893184
author Molazemhosseini, Alireza
Magagnin, Luca
Vena, Pasquale
Liu, Chung-Chiun
author_facet Molazemhosseini, Alireza
Magagnin, Luca
Vena, Pasquale
Liu, Chung-Chiun
author_sort Molazemhosseini, Alireza
collection PubMed
description A single-use disposable in vitro electrochemical immunosensor for the detection of HbA1c in undiluted human serum using differential pulse voltammetry (DPV) was developed. A three-electrode configuration electrochemical biosensor consisted of 10-nm-thin gold film working and counter electrodes and a thick-film printed Ag/AgCl reference electrode was fabricated on a polyethylene terephthalate (PET) substrate. Micro-fabrication techniques including sputtering vapor deposition and thick-film printing were used to fabricate the biosensor. This was a roll-to-roll cost-effective manufacturing process making the single-use disposable in vitro HbA1c biosensor a reality. Self-assembled monolayers of 3-Mercaptopropionic acid (MPA) were employed to covalently immobilize anti-HbA1c on the surface of gold electrodes. Electrochemical impedance spectroscopy (EIS) and X-ray photoelectron spectroscopy (XPS) confirmed the excellent coverage of MPA-SAM and the upward orientation of carboxylic groups. The hindering effect of HbA1c on the ferricyanide/ferrocyanide electron transfer reaction was exploited as the HbA1c detection mechanism. The biosensor showed a linear range of 7.5–20 µg/mL of HbA1c in 0.1 M PBS. Using undiluted human serum as the test medium, the biosensor presented an excellent linear behavior (R(2) = 0.999) in the range of 0.1–0.25 mg/mL of HbA1c. The potential application of this biosensor for in vitro measurement of HbA1c for diabetic management was demonstrated.
format Online
Article
Text
id pubmed-4970074
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-49700742016-08-04 Single-Use Disposable Electrochemical Label-Free Immunosensor for Detection of Glycated Hemoglobin (HbA1c) Using Differential Pulse Voltammetry (DPV) Molazemhosseini, Alireza Magagnin, Luca Vena, Pasquale Liu, Chung-Chiun Sensors (Basel) Article A single-use disposable in vitro electrochemical immunosensor for the detection of HbA1c in undiluted human serum using differential pulse voltammetry (DPV) was developed. A three-electrode configuration electrochemical biosensor consisted of 10-nm-thin gold film working and counter electrodes and a thick-film printed Ag/AgCl reference electrode was fabricated on a polyethylene terephthalate (PET) substrate. Micro-fabrication techniques including sputtering vapor deposition and thick-film printing were used to fabricate the biosensor. This was a roll-to-roll cost-effective manufacturing process making the single-use disposable in vitro HbA1c biosensor a reality. Self-assembled monolayers of 3-Mercaptopropionic acid (MPA) were employed to covalently immobilize anti-HbA1c on the surface of gold electrodes. Electrochemical impedance spectroscopy (EIS) and X-ray photoelectron spectroscopy (XPS) confirmed the excellent coverage of MPA-SAM and the upward orientation of carboxylic groups. The hindering effect of HbA1c on the ferricyanide/ferrocyanide electron transfer reaction was exploited as the HbA1c detection mechanism. The biosensor showed a linear range of 7.5–20 µg/mL of HbA1c in 0.1 M PBS. Using undiluted human serum as the test medium, the biosensor presented an excellent linear behavior (R(2) = 0.999) in the range of 0.1–0.25 mg/mL of HbA1c. The potential application of this biosensor for in vitro measurement of HbA1c for diabetic management was demonstrated. MDPI 2016-07-01 /pmc/articles/PMC4970074/ /pubmed/27376299 http://dx.doi.org/10.3390/s16071024 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Molazemhosseini, Alireza
Magagnin, Luca
Vena, Pasquale
Liu, Chung-Chiun
Single-Use Disposable Electrochemical Label-Free Immunosensor for Detection of Glycated Hemoglobin (HbA1c) Using Differential Pulse Voltammetry (DPV)
title Single-Use Disposable Electrochemical Label-Free Immunosensor for Detection of Glycated Hemoglobin (HbA1c) Using Differential Pulse Voltammetry (DPV)
title_full Single-Use Disposable Electrochemical Label-Free Immunosensor for Detection of Glycated Hemoglobin (HbA1c) Using Differential Pulse Voltammetry (DPV)
title_fullStr Single-Use Disposable Electrochemical Label-Free Immunosensor for Detection of Glycated Hemoglobin (HbA1c) Using Differential Pulse Voltammetry (DPV)
title_full_unstemmed Single-Use Disposable Electrochemical Label-Free Immunosensor for Detection of Glycated Hemoglobin (HbA1c) Using Differential Pulse Voltammetry (DPV)
title_short Single-Use Disposable Electrochemical Label-Free Immunosensor for Detection of Glycated Hemoglobin (HbA1c) Using Differential Pulse Voltammetry (DPV)
title_sort single-use disposable electrochemical label-free immunosensor for detection of glycated hemoglobin (hba1c) using differential pulse voltammetry (dpv)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4970074/
https://www.ncbi.nlm.nih.gov/pubmed/27376299
http://dx.doi.org/10.3390/s16071024
work_keys_str_mv AT molazemhosseinialireza singleusedisposableelectrochemicallabelfreeimmunosensorfordetectionofglycatedhemoglobinhba1cusingdifferentialpulsevoltammetrydpv
AT magagninluca singleusedisposableelectrochemicallabelfreeimmunosensorfordetectionofglycatedhemoglobinhba1cusingdifferentialpulsevoltammetrydpv
AT venapasquale singleusedisposableelectrochemicallabelfreeimmunosensorfordetectionofglycatedhemoglobinhba1cusingdifferentialpulsevoltammetrydpv
AT liuchungchiun singleusedisposableelectrochemicallabelfreeimmunosensorfordetectionofglycatedhemoglobinhba1cusingdifferentialpulsevoltammetrydpv