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Novel role for the midbody in primary ciliogenesis by polarized epithelial cells
The primary cilium is a membrane protrusion that is crucial for vertebrate tissue homeostasis and development. Here, we investigated the uncharacterized process of primary ciliogenesis in polarized epithelial cells. We show that after cytokinesis, the midbody is inherited by one of the daughter cell...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4970324/ https://www.ncbi.nlm.nih.gov/pubmed/27458130 http://dx.doi.org/10.1083/jcb.201601020 |
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author | Bernabé-Rubio, Miguel Andrés, Germán Casares-Arias, Javier Fernández-Barrera, Jaime Rangel, Laura Reglero-Real, Natalia Gershlick, David C. Fernández, José J. Millán, Jaime Correas, Isabel Miguez, David G. Alonso, Miguel A. |
author_facet | Bernabé-Rubio, Miguel Andrés, Germán Casares-Arias, Javier Fernández-Barrera, Jaime Rangel, Laura Reglero-Real, Natalia Gershlick, David C. Fernández, José J. Millán, Jaime Correas, Isabel Miguez, David G. Alonso, Miguel A. |
author_sort | Bernabé-Rubio, Miguel |
collection | PubMed |
description | The primary cilium is a membrane protrusion that is crucial for vertebrate tissue homeostasis and development. Here, we investigated the uncharacterized process of primary ciliogenesis in polarized epithelial cells. We show that after cytokinesis, the midbody is inherited by one of the daughter cells as a remnant that initially locates peripherally at the apical surface of one of the daughter cells. The remnant then moves along the apical surface and, once proximal to the centrosome at the center of the apical surface, enables cilium formation. The physical removal of the remnant greatly impairs ciliogenesis. We developed a probabilistic cell population–based model that reproduces the experimental data. In addition, our model explains, solely in terms of cell area constraints, the various observed transitions of the midbody, the beginning of ciliogenesis, and the accumulation of ciliated cells. Our findings reveal a biological mechanism that links the three microtubule-based organelles—the midbody, the centrosome, and the cilium—in the same cellular process. |
format | Online Article Text |
id | pubmed-4970324 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-49703242017-02-01 Novel role for the midbody in primary ciliogenesis by polarized epithelial cells Bernabé-Rubio, Miguel Andrés, Germán Casares-Arias, Javier Fernández-Barrera, Jaime Rangel, Laura Reglero-Real, Natalia Gershlick, David C. Fernández, José J. Millán, Jaime Correas, Isabel Miguez, David G. Alonso, Miguel A. J Cell Biol Research Articles The primary cilium is a membrane protrusion that is crucial for vertebrate tissue homeostasis and development. Here, we investigated the uncharacterized process of primary ciliogenesis in polarized epithelial cells. We show that after cytokinesis, the midbody is inherited by one of the daughter cells as a remnant that initially locates peripherally at the apical surface of one of the daughter cells. The remnant then moves along the apical surface and, once proximal to the centrosome at the center of the apical surface, enables cilium formation. The physical removal of the remnant greatly impairs ciliogenesis. We developed a probabilistic cell population–based model that reproduces the experimental data. In addition, our model explains, solely in terms of cell area constraints, the various observed transitions of the midbody, the beginning of ciliogenesis, and the accumulation of ciliated cells. Our findings reveal a biological mechanism that links the three microtubule-based organelles—the midbody, the centrosome, and the cilium—in the same cellular process. The Rockefeller University Press 2016-08-01 /pmc/articles/PMC4970324/ /pubmed/27458130 http://dx.doi.org/10.1083/jcb.201601020 Text en © 2016 Bernabé-Rubio et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Bernabé-Rubio, Miguel Andrés, Germán Casares-Arias, Javier Fernández-Barrera, Jaime Rangel, Laura Reglero-Real, Natalia Gershlick, David C. Fernández, José J. Millán, Jaime Correas, Isabel Miguez, David G. Alonso, Miguel A. Novel role for the midbody in primary ciliogenesis by polarized epithelial cells |
title | Novel role for the midbody in primary ciliogenesis by polarized epithelial cells |
title_full | Novel role for the midbody in primary ciliogenesis by polarized epithelial cells |
title_fullStr | Novel role for the midbody in primary ciliogenesis by polarized epithelial cells |
title_full_unstemmed | Novel role for the midbody in primary ciliogenesis by polarized epithelial cells |
title_short | Novel role for the midbody in primary ciliogenesis by polarized epithelial cells |
title_sort | novel role for the midbody in primary ciliogenesis by polarized epithelial cells |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4970324/ https://www.ncbi.nlm.nih.gov/pubmed/27458130 http://dx.doi.org/10.1083/jcb.201601020 |
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