Method development and validation of potent pyrimidine derivative by UV-VIS spectrophotometer

BACKGROUND: A rapid and sensitive ultraviolet-visible (UV-VIS) spectroscopic method was developed for the estimation of pyrimidine derivative 6-Bromo-3-(6-(2,6-dichlorophenyl)-2-(morpolinomethylamino) pyrimidine4-yl) -2H-chromen-2-one (BT(10)M) in bulk form. RESULTS: Pyrimidine derivative was monitored at 275 nm with UV detection, and there is no interference of diluents at 275 nm. The method was found to be linear in the range of 50 to 150 μg/ml. The accuracy and precision were determined and validated statistically. The method was validated as a guideline. CONCLUSIONS: The results showed that the proposed method is suitable for the accurate, precise, and rapid determination of pyrimidine derivative. [Figure: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13588-014-0015-9) contains supplementary material, which is available to authorized users.