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Trichoderma Biodiversity of Agricultural Fields in East China Reveals a Gradient Distribution of Species

We surveyed the Trichoderma (Hypocreales, Ascomycota) biodiversity in agricultural fields in four major agricultural provinces of East China. Trichoderma strains were identified based on molecular approaches and morphological characteristics. In three sampled seasons (spring, summer and autumn), 207...

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Detalles Bibliográficos
Autores principales: Jiang, Yuan, Wang, Jin-Liang, Chen, Jing, Mao, Li-Juan, Feng, Xiao-Xiao, Zhang, Chu-Long, Lin, Fu-Cheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4970770/
https://www.ncbi.nlm.nih.gov/pubmed/27482910
http://dx.doi.org/10.1371/journal.pone.0160613
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author Jiang, Yuan
Wang, Jin-Liang
Chen, Jing
Mao, Li-Juan
Feng, Xiao-Xiao
Zhang, Chu-Long
Lin, Fu-Cheng
author_facet Jiang, Yuan
Wang, Jin-Liang
Chen, Jing
Mao, Li-Juan
Feng, Xiao-Xiao
Zhang, Chu-Long
Lin, Fu-Cheng
author_sort Jiang, Yuan
collection PubMed
description We surveyed the Trichoderma (Hypocreales, Ascomycota) biodiversity in agricultural fields in four major agricultural provinces of East China. Trichoderma strains were identified based on molecular approaches and morphological characteristics. In three sampled seasons (spring, summer and autumn), 2078 strains were isolated and identified to 17 known species: T. harzianum (429 isolates), T. asperellum (425), T. hamatum (397), T. virens (340), T. koningiopsis (248), T. brevicompactum (73), T. atroviride (73), T. fertile (26), T. longibrachiatum (22), T. pleuroticola (16), T. erinaceum (16), T. oblongisporum (2), T. polysporum (2), T. spirale (2), T. capillare (2), T. velutinum (2), and T. saturnisporum (1). T. harzianum, T. asperellum, T. hamatum, and T. virens were identified as the dominant species with dominance (Y) values of 0.057, 0.052, 0.048, and 0.039, respectively. The species amount, isolate numbers and the dominant species of Trichoderma varied between provinces. Zhejiang Province has shown the highest diversity, which was reflected in the highest species amount (14) and the highest Shannon–Wiener diversity index of Trichoderma haplotypes (1.46). We observed that relative frequencies of T. hamatum and T. koningiopsis under rice soil were higher than those under wheat and maize soil, indicating the preference of Trichoderma to different crops. Remarkable seasonal variation was shown, with summer exhibiting the highest biodiversity of the studied seasons. These results show that Trichoderma biodiversity in agricultural fields varies by region, crop, and season. Zhejiang Province (the southernmost province in the investigated area) had more T. hamatum than Shandong Province (the northernmost province), not only in isolate amounts but also in haplotype amounts. Furthermore, at haplotype level, only T. hamatum showed a gradient distribution from south to north in correspondence analysis among the four dominant species. The above results would contribute to the application of Trichoderma biocontrol strains.
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spelling pubmed-49707702016-08-18 Trichoderma Biodiversity of Agricultural Fields in East China Reveals a Gradient Distribution of Species Jiang, Yuan Wang, Jin-Liang Chen, Jing Mao, Li-Juan Feng, Xiao-Xiao Zhang, Chu-Long Lin, Fu-Cheng PLoS One Research Article We surveyed the Trichoderma (Hypocreales, Ascomycota) biodiversity in agricultural fields in four major agricultural provinces of East China. Trichoderma strains were identified based on molecular approaches and morphological characteristics. In three sampled seasons (spring, summer and autumn), 2078 strains were isolated and identified to 17 known species: T. harzianum (429 isolates), T. asperellum (425), T. hamatum (397), T. virens (340), T. koningiopsis (248), T. brevicompactum (73), T. atroviride (73), T. fertile (26), T. longibrachiatum (22), T. pleuroticola (16), T. erinaceum (16), T. oblongisporum (2), T. polysporum (2), T. spirale (2), T. capillare (2), T. velutinum (2), and T. saturnisporum (1). T. harzianum, T. asperellum, T. hamatum, and T. virens were identified as the dominant species with dominance (Y) values of 0.057, 0.052, 0.048, and 0.039, respectively. The species amount, isolate numbers and the dominant species of Trichoderma varied between provinces. Zhejiang Province has shown the highest diversity, which was reflected in the highest species amount (14) and the highest Shannon–Wiener diversity index of Trichoderma haplotypes (1.46). We observed that relative frequencies of T. hamatum and T. koningiopsis under rice soil were higher than those under wheat and maize soil, indicating the preference of Trichoderma to different crops. Remarkable seasonal variation was shown, with summer exhibiting the highest biodiversity of the studied seasons. These results show that Trichoderma biodiversity in agricultural fields varies by region, crop, and season. Zhejiang Province (the southernmost province in the investigated area) had more T. hamatum than Shandong Province (the northernmost province), not only in isolate amounts but also in haplotype amounts. Furthermore, at haplotype level, only T. hamatum showed a gradient distribution from south to north in correspondence analysis among the four dominant species. The above results would contribute to the application of Trichoderma biocontrol strains. Public Library of Science 2016-08-02 /pmc/articles/PMC4970770/ /pubmed/27482910 http://dx.doi.org/10.1371/journal.pone.0160613 Text en © 2016 Jiang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Jiang, Yuan
Wang, Jin-Liang
Chen, Jing
Mao, Li-Juan
Feng, Xiao-Xiao
Zhang, Chu-Long
Lin, Fu-Cheng
Trichoderma Biodiversity of Agricultural Fields in East China Reveals a Gradient Distribution of Species
title Trichoderma Biodiversity of Agricultural Fields in East China Reveals a Gradient Distribution of Species
title_full Trichoderma Biodiversity of Agricultural Fields in East China Reveals a Gradient Distribution of Species
title_fullStr Trichoderma Biodiversity of Agricultural Fields in East China Reveals a Gradient Distribution of Species
title_full_unstemmed Trichoderma Biodiversity of Agricultural Fields in East China Reveals a Gradient Distribution of Species
title_short Trichoderma Biodiversity of Agricultural Fields in East China Reveals a Gradient Distribution of Species
title_sort trichoderma biodiversity of agricultural fields in east china reveals a gradient distribution of species
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4970770/
https://www.ncbi.nlm.nih.gov/pubmed/27482910
http://dx.doi.org/10.1371/journal.pone.0160613
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