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Development and Maturation of Embryonic Cortical Neurons Grafted into the Damaged Adult Motor Cortex
Injury to the human central nervous system can lead to devastating consequences due to its poor ability to self-repair. Neural transplantation aimed at replacing lost neurons and restore functional circuitry has proven to be a promising therapeutical avenue. We previously reported in adult rodent an...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4971105/ https://www.ncbi.nlm.nih.gov/pubmed/27536221 http://dx.doi.org/10.3389/fncir.2016.00055 |
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author | Ballout, Nissrine Frappé, Isabelle Péron, Sophie Jaber, Mohamed Zibara, Kazem Gaillard, Afsaneh |
author_facet | Ballout, Nissrine Frappé, Isabelle Péron, Sophie Jaber, Mohamed Zibara, Kazem Gaillard, Afsaneh |
author_sort | Ballout, Nissrine |
collection | PubMed |
description | Injury to the human central nervous system can lead to devastating consequences due to its poor ability to self-repair. Neural transplantation aimed at replacing lost neurons and restore functional circuitry has proven to be a promising therapeutical avenue. We previously reported in adult rodent animal models with cortical lesions that grafted fetal cortical neurons could effectively re-establish specific patterns of projections and synapses. The current study was designed to provide a detailed characterization of the spatio-temporal in vivo development of fetal cortical transplanted cells within the lesioned adult motor cortex and their corresponding axonal projections. We show here that as early as 2 weeks after grafting, cortical neuroblasts transplanted into damaged adult motor cortex developed appropriate projections to cortical and subcortical targets. Grafted cells initially exhibited characteristics of immature neurons, which then differentiated into mature neurons with appropriate cortical phenotypes where most were glutamatergic and few were GABAergic. All cortical subtypes identified with the specific markers CTIP2, Cux1, FOXP2, and Tbr1 were generated after grafting as evidenced with BrdU co-labeling. The set of data provided here is of interest as it sets biological standards for future studies aimed at replacing fetal cells with embryonic stem cells as a source of cortical neurons. |
format | Online Article Text |
id | pubmed-4971105 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-49711052016-08-17 Development and Maturation of Embryonic Cortical Neurons Grafted into the Damaged Adult Motor Cortex Ballout, Nissrine Frappé, Isabelle Péron, Sophie Jaber, Mohamed Zibara, Kazem Gaillard, Afsaneh Front Neural Circuits Neuroscience Injury to the human central nervous system can lead to devastating consequences due to its poor ability to self-repair. Neural transplantation aimed at replacing lost neurons and restore functional circuitry has proven to be a promising therapeutical avenue. We previously reported in adult rodent animal models with cortical lesions that grafted fetal cortical neurons could effectively re-establish specific patterns of projections and synapses. The current study was designed to provide a detailed characterization of the spatio-temporal in vivo development of fetal cortical transplanted cells within the lesioned adult motor cortex and their corresponding axonal projections. We show here that as early as 2 weeks after grafting, cortical neuroblasts transplanted into damaged adult motor cortex developed appropriate projections to cortical and subcortical targets. Grafted cells initially exhibited characteristics of immature neurons, which then differentiated into mature neurons with appropriate cortical phenotypes where most were glutamatergic and few were GABAergic. All cortical subtypes identified with the specific markers CTIP2, Cux1, FOXP2, and Tbr1 were generated after grafting as evidenced with BrdU co-labeling. The set of data provided here is of interest as it sets biological standards for future studies aimed at replacing fetal cells with embryonic stem cells as a source of cortical neurons. Frontiers Media S.A. 2016-08-03 /pmc/articles/PMC4971105/ /pubmed/27536221 http://dx.doi.org/10.3389/fncir.2016.00055 Text en Copyright © 2016 Ballout, Frappé, Péron, Jaber, Zibara and Gaillard. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neuroscience Ballout, Nissrine Frappé, Isabelle Péron, Sophie Jaber, Mohamed Zibara, Kazem Gaillard, Afsaneh Development and Maturation of Embryonic Cortical Neurons Grafted into the Damaged Adult Motor Cortex |
title | Development and Maturation of Embryonic Cortical Neurons Grafted into the Damaged Adult Motor Cortex |
title_full | Development and Maturation of Embryonic Cortical Neurons Grafted into the Damaged Adult Motor Cortex |
title_fullStr | Development and Maturation of Embryonic Cortical Neurons Grafted into the Damaged Adult Motor Cortex |
title_full_unstemmed | Development and Maturation of Embryonic Cortical Neurons Grafted into the Damaged Adult Motor Cortex |
title_short | Development and Maturation of Embryonic Cortical Neurons Grafted into the Damaged Adult Motor Cortex |
title_sort | development and maturation of embryonic cortical neurons grafted into the damaged adult motor cortex |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4971105/ https://www.ncbi.nlm.nih.gov/pubmed/27536221 http://dx.doi.org/10.3389/fncir.2016.00055 |
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