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2-cyclohexylamino-5,8-dimethoxy-1,4-naphthoquinone inhibits LPS-induced BV2 microglial activation through MAPK/NF-kB signaling pathways

AIMS: To verify the effects of several 5,8-dimethoxy-1,4-naphthoquinone (DMNQ) derivatives on LPS-induced NO production, cellular ROS levels and cytokine expression in BV-2 microglial cells. MAIN METHODS: An MTT assay and FACS flow cytometry were performed to assess the cellular viability and apopto...

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Autores principales: Sun, Hu-Nan, Shen, Gui-Nan, Jin, Yong-Zhe, Jin, Yu, Han, Ying-Hao, Feng, Li, Liu, Lei, Jin, Mei-Hua, Luo, Ying-Hua, Kwon, Tea-Ho, Cui, Yu-Dong, Jin, Cheng-Hao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4971128/
https://www.ncbi.nlm.nih.gov/pubmed/27512726
http://dx.doi.org/10.1016/j.heliyon.2016.e00132
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author Sun, Hu-Nan
Shen, Gui-Nan
Jin, Yong-Zhe
Jin, Yu
Han, Ying-Hao
Feng, Li
Liu, Lei
Jin, Mei-Hua
Luo, Ying-Hua
Kwon, Tea-Ho
Cui, Yu-Dong
Jin, Cheng-Hao
author_facet Sun, Hu-Nan
Shen, Gui-Nan
Jin, Yong-Zhe
Jin, Yu
Han, Ying-Hao
Feng, Li
Liu, Lei
Jin, Mei-Hua
Luo, Ying-Hua
Kwon, Tea-Ho
Cui, Yu-Dong
Jin, Cheng-Hao
author_sort Sun, Hu-Nan
collection PubMed
description AIMS: To verify the effects of several 5,8-dimethoxy-1,4-naphthoquinone (DMNQ) derivatives on LPS-induced NO production, cellular ROS levels and cytokine expression in BV-2 microglial cells. MAIN METHODS: An MTT assay and FACS flow cytometry were performed to assess the cellular viability and apoptosis and cellular ROS levels, respectively. To examine the expression of pro-inflammatory cytokines and cellular signaling pathways, semi-quantitative RT-PCR and Western blotting were also used in this study. KEY FINDINGS: Among the six newly synthesized DMNQ derivatives, 2-cyclohexylamino-5,8-dimethoxy-1,4-naphthoquinone (R6) significantly inhibited the NO production, cellular ROS levels and the cytokines expression in BV-2 microglial cells, which stimulated by LPS. Signaling study showed that compound R6 treatment also significantly down-regulated the LPS-induced phosphorylation of MAPKs (ERK, JNK and p38) and decreased the degradation of IκB-α in BV2 microglial cells. SIGNIFICANCE: Our findings demonstrate that our newly synthesized compound derived from DMNQ, 2-cyclohexylamino-5,8-dimethoxy-1,4-naphthoquinone (R6), might be a therapeutic agent for the treatment of glia-mediated neuroinflammatory diseases.
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spelling pubmed-49711282016-08-10 2-cyclohexylamino-5,8-dimethoxy-1,4-naphthoquinone inhibits LPS-induced BV2 microglial activation through MAPK/NF-kB signaling pathways Sun, Hu-Nan Shen, Gui-Nan Jin, Yong-Zhe Jin, Yu Han, Ying-Hao Feng, Li Liu, Lei Jin, Mei-Hua Luo, Ying-Hua Kwon, Tea-Ho Cui, Yu-Dong Jin, Cheng-Hao Heliyon Article AIMS: To verify the effects of several 5,8-dimethoxy-1,4-naphthoquinone (DMNQ) derivatives on LPS-induced NO production, cellular ROS levels and cytokine expression in BV-2 microglial cells. MAIN METHODS: An MTT assay and FACS flow cytometry were performed to assess the cellular viability and apoptosis and cellular ROS levels, respectively. To examine the expression of pro-inflammatory cytokines and cellular signaling pathways, semi-quantitative RT-PCR and Western blotting were also used in this study. KEY FINDINGS: Among the six newly synthesized DMNQ derivatives, 2-cyclohexylamino-5,8-dimethoxy-1,4-naphthoquinone (R6) significantly inhibited the NO production, cellular ROS levels and the cytokines expression in BV-2 microglial cells, which stimulated by LPS. Signaling study showed that compound R6 treatment also significantly down-regulated the LPS-induced phosphorylation of MAPKs (ERK, JNK and p38) and decreased the degradation of IκB-α in BV2 microglial cells. SIGNIFICANCE: Our findings demonstrate that our newly synthesized compound derived from DMNQ, 2-cyclohexylamino-5,8-dimethoxy-1,4-naphthoquinone (R6), might be a therapeutic agent for the treatment of glia-mediated neuroinflammatory diseases. Elsevier 2016-07-20 /pmc/articles/PMC4971128/ /pubmed/27512726 http://dx.doi.org/10.1016/j.heliyon.2016.e00132 Text en © 2016 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Sun, Hu-Nan
Shen, Gui-Nan
Jin, Yong-Zhe
Jin, Yu
Han, Ying-Hao
Feng, Li
Liu, Lei
Jin, Mei-Hua
Luo, Ying-Hua
Kwon, Tea-Ho
Cui, Yu-Dong
Jin, Cheng-Hao
2-cyclohexylamino-5,8-dimethoxy-1,4-naphthoquinone inhibits LPS-induced BV2 microglial activation through MAPK/NF-kB signaling pathways
title 2-cyclohexylamino-5,8-dimethoxy-1,4-naphthoquinone inhibits LPS-induced BV2 microglial activation through MAPK/NF-kB signaling pathways
title_full 2-cyclohexylamino-5,8-dimethoxy-1,4-naphthoquinone inhibits LPS-induced BV2 microglial activation through MAPK/NF-kB signaling pathways
title_fullStr 2-cyclohexylamino-5,8-dimethoxy-1,4-naphthoquinone inhibits LPS-induced BV2 microglial activation through MAPK/NF-kB signaling pathways
title_full_unstemmed 2-cyclohexylamino-5,8-dimethoxy-1,4-naphthoquinone inhibits LPS-induced BV2 microglial activation through MAPK/NF-kB signaling pathways
title_short 2-cyclohexylamino-5,8-dimethoxy-1,4-naphthoquinone inhibits LPS-induced BV2 microglial activation through MAPK/NF-kB signaling pathways
title_sort 2-cyclohexylamino-5,8-dimethoxy-1,4-naphthoquinone inhibits lps-induced bv2 microglial activation through mapk/nf-kb signaling pathways
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4971128/
https://www.ncbi.nlm.nih.gov/pubmed/27512726
http://dx.doi.org/10.1016/j.heliyon.2016.e00132
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