Fusion to a homo-oligomeric scaffold allows cryo-EM analysis of a small protein

Recent technical advances have revolutionized the field of cryo-electron microscopy (cryo-EM). However, most monomeric proteins remain too small (<100 kDa) for cryo-EM analysis. To overcome this limitation, we explored a strategy whereby a monomeric target protein is genetically fused to a homo-o...

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Detalles Bibliográficos
Autores principales: Coscia, Francesca, Estrozi, Leandro F., Hans, Fabienne, Malet, Hélène, Noirclerc-Savoye, Marjolaine, Schoehn, Guy, Petosa, Carlo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4971460/
https://www.ncbi.nlm.nih.gov/pubmed/27485862
http://dx.doi.org/10.1038/srep30909

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