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A simultaneous diagnosis and genotyping method for global surveillance of cetacean morbillivirus
Cetacean morbillivirus (CeMV) is considered one of the most important viral pathogens in cetaceans. CeMV outbreaks of lethal disease have repeatedly been observed in Europe, the Americas, and Australia, while large herds of gregarious species were found to be the likely reservoirs and sources of CeM...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4971491/ https://www.ncbi.nlm.nih.gov/pubmed/27484954 http://dx.doi.org/10.1038/srep30625 |
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author | Yang, Wei-Cheng Wu, Bi-Jhen Sierra, Eva Fernandez, Antonio Groch, Kátia R. Catão-Dias, José Luiz West, Kristi Chan, Kun-Wei |
author_facet | Yang, Wei-Cheng Wu, Bi-Jhen Sierra, Eva Fernandez, Antonio Groch, Kátia R. Catão-Dias, José Luiz West, Kristi Chan, Kun-Wei |
author_sort | Yang, Wei-Cheng |
collection | PubMed |
description | Cetacean morbillivirus (CeMV) is considered one of the most important viral pathogens in cetaceans. CeMV outbreaks of lethal disease have repeatedly been observed in Europe, the Americas, and Australia, while large herds of gregarious species were found to be the likely reservoirs and sources of CeMV infection to susceptible species in the Atlantic and Pacific Oceans. Furthermore, three new strains were detected recently in Hawaii, Brazil and Australia. To clarify the real global distribution of CeMV and possible carriers, we showed a novel technique successfully diagnosing and distinguishing different virus strains (DMV, PWMV and novel CeMVs) using FFPE samples from 1996 to 2011. This efficient method that combines qRT-PCR and high resolution melting (HRM) could be applied to the future retrospective global studies for better understanding of different prevalence and outbreak conditions among ocean basins and the mechanism of variable host response to pathogens. |
format | Online Article Text |
id | pubmed-4971491 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-49714912016-08-11 A simultaneous diagnosis and genotyping method for global surveillance of cetacean morbillivirus Yang, Wei-Cheng Wu, Bi-Jhen Sierra, Eva Fernandez, Antonio Groch, Kátia R. Catão-Dias, José Luiz West, Kristi Chan, Kun-Wei Sci Rep Article Cetacean morbillivirus (CeMV) is considered one of the most important viral pathogens in cetaceans. CeMV outbreaks of lethal disease have repeatedly been observed in Europe, the Americas, and Australia, while large herds of gregarious species were found to be the likely reservoirs and sources of CeMV infection to susceptible species in the Atlantic and Pacific Oceans. Furthermore, three new strains were detected recently in Hawaii, Brazil and Australia. To clarify the real global distribution of CeMV and possible carriers, we showed a novel technique successfully diagnosing and distinguishing different virus strains (DMV, PWMV and novel CeMVs) using FFPE samples from 1996 to 2011. This efficient method that combines qRT-PCR and high resolution melting (HRM) could be applied to the future retrospective global studies for better understanding of different prevalence and outbreak conditions among ocean basins and the mechanism of variable host response to pathogens. Nature Publishing Group 2016-08-03 /pmc/articles/PMC4971491/ /pubmed/27484954 http://dx.doi.org/10.1038/srep30625 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Yang, Wei-Cheng Wu, Bi-Jhen Sierra, Eva Fernandez, Antonio Groch, Kátia R. Catão-Dias, José Luiz West, Kristi Chan, Kun-Wei A simultaneous diagnosis and genotyping method for global surveillance of cetacean morbillivirus |
title | A simultaneous diagnosis and genotyping method for global surveillance of cetacean morbillivirus |
title_full | A simultaneous diagnosis and genotyping method for global surveillance of cetacean morbillivirus |
title_fullStr | A simultaneous diagnosis and genotyping method for global surveillance of cetacean morbillivirus |
title_full_unstemmed | A simultaneous diagnosis and genotyping method for global surveillance of cetacean morbillivirus |
title_short | A simultaneous diagnosis and genotyping method for global surveillance of cetacean morbillivirus |
title_sort | simultaneous diagnosis and genotyping method for global surveillance of cetacean morbillivirus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4971491/ https://www.ncbi.nlm.nih.gov/pubmed/27484954 http://dx.doi.org/10.1038/srep30625 |
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