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Canine distemper virus isolated from a monkey efficiently replicates on Vero cells expressing non-human primate SLAM receptors but not human SLAM receptor

BACKGROUND: In 2008, an outbreak of canine distemper virus (CDV) infection in monkeys was reported in China. We isolated CDV strain (subsequently named Monkey-BJ01-DV) from lung tissue obtained from a rhesus monkey that died in this outbreak. We evaluated the ability of this virus on Vero cells expr...

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Detalles Bibliográficos
Autores principales: Feng, Na, Liu, Yuxiu, Wang, Jianzhong, Xu, Weiwei, Li, Tiansong, Wang, Tiecheng, Wang, Lei, Yu, Yicong, Wang, Hualei, Zhao, Yongkun, Yang, Songtao, Gao, Yuwei, Hu, Guixue, Xia, Xianzhu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4971657/
https://www.ncbi.nlm.nih.gov/pubmed/27484638
http://dx.doi.org/10.1186/s12917-016-0757-x
Descripción
Sumario:BACKGROUND: In 2008, an outbreak of canine distemper virus (CDV) infection in monkeys was reported in China. We isolated CDV strain (subsequently named Monkey-BJ01-DV) from lung tissue obtained from a rhesus monkey that died in this outbreak. We evaluated the ability of this virus on Vero cells expressing SLAM receptors from dog, monkey and human origin, and analyzed the H gene of Monkey-BJ01-DV with other strains. RESULTS: The Monkey-BJ01-DV isolate replicated to the highest titer on Vero cells expressing dog-origin SLAM (10(5.2±0.2) TCID(50)/ml) and monkey-origin SLAM (10(5.4±0.1) TCID(50)/ml), but achieved markedly lower titers on human-origin SLAM cells (10(3.3±0.3) TCID(50)/ml). Phylogenetic analysis of the full-length H gene showed that Monkey-BJ01-DV was highly related to other CDV strains obtained during recent CDV epidemics among species of the Canidae family in China, and these Monkey strains CDV (Monkey-BJ01-DV, CYN07-dV, Monkey-KM-01) possessed a number of amino acid specific substitutions (E276V, Q392R, D435Y and I542F) compared to the H protein of CDV epidemic in other animals at the same period. CONCLUSIONS: Our results suggested that the monkey origin-CDV-H protein could possess specific substitutions to adapt to the new host. Monkey-BJ01-DV can efficiently use monkey- and dog-origin SLAM to infect and replicate in host cells, but further adaptation may be required for efficient replication in host cells expressing the human SLAM receptor.