Cargando…
NMR Study Reveals the Receiver Domain of Arabidopsis ETHYLENE RESPONSE1 Ethylene Receptor as an Atypical Type Response Regulator
The gaseous plant hormone ethylene, recognized by plant ethylene receptors, plays a pivotal role in various aspects of plant growth and development. ETHYLENE RESPONSE1 (ETR1) is an ethylene receptor isolated from Arabidopsis and has a structure characteristic of prokaryotic two-component histidine k...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4972365/ https://www.ncbi.nlm.nih.gov/pubmed/27486797 http://dx.doi.org/10.1371/journal.pone.0160598 |
_version_ | 1782446233464340480 |
---|---|
author | Hung, Yi-Lin Jiang, Ingjye Lee, Yi-Zong Wen, Chi-Kuang Sue, Shih-Che |
author_facet | Hung, Yi-Lin Jiang, Ingjye Lee, Yi-Zong Wen, Chi-Kuang Sue, Shih-Che |
author_sort | Hung, Yi-Lin |
collection | PubMed |
description | The gaseous plant hormone ethylene, recognized by plant ethylene receptors, plays a pivotal role in various aspects of plant growth and development. ETHYLENE RESPONSE1 (ETR1) is an ethylene receptor isolated from Arabidopsis and has a structure characteristic of prokaryotic two-component histidine kinase (HK) and receiver domain (RD), where the RD structurally resembles bacteria response regulators (RRs). The ETR1 HK domain has autophosphorylation activity, and little is known if the HK can transfer the phosphoryl group to the RD for receptor signaling. Unveiling the correlation of the receptor structure and phosphorylation status would advance the studies towards the underlying mechanisms of ETR1 receptor signaling. In this study, using the nuclear magnetic resonance technique, our data suggested that the ETR1-RD is monomeric in solution and the rigid structure of the RD prevents the conserved aspartate residue phosphorylation. Comparing the backbone dynamics with other RRs, we propose that backbone flexibility is critical to the RR phosphorylation. Besides the limited flexibility, ETR1-RD has a unique γ loop conformation of opposite orientation, which makes ETR1-RD unfavorable for phosphorylation. These two features explain why ETR1-RD cannot be phosphorylated and is classified as an atypical type RR. As a control, phosphorylation of the ETR1-RD was also impaired when the sequence was swapped to the fragment of the bacterial typical type RR, CheY. Here, we suggest a molecule insight that the ETR1-RD already exists as an active formation and executes its function through binding with the downstream factors without phosphorylation. |
format | Online Article Text |
id | pubmed-4972365 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-49723652016-08-18 NMR Study Reveals the Receiver Domain of Arabidopsis ETHYLENE RESPONSE1 Ethylene Receptor as an Atypical Type Response Regulator Hung, Yi-Lin Jiang, Ingjye Lee, Yi-Zong Wen, Chi-Kuang Sue, Shih-Che PLoS One Research Article The gaseous plant hormone ethylene, recognized by plant ethylene receptors, plays a pivotal role in various aspects of plant growth and development. ETHYLENE RESPONSE1 (ETR1) is an ethylene receptor isolated from Arabidopsis and has a structure characteristic of prokaryotic two-component histidine kinase (HK) and receiver domain (RD), where the RD structurally resembles bacteria response regulators (RRs). The ETR1 HK domain has autophosphorylation activity, and little is known if the HK can transfer the phosphoryl group to the RD for receptor signaling. Unveiling the correlation of the receptor structure and phosphorylation status would advance the studies towards the underlying mechanisms of ETR1 receptor signaling. In this study, using the nuclear magnetic resonance technique, our data suggested that the ETR1-RD is monomeric in solution and the rigid structure of the RD prevents the conserved aspartate residue phosphorylation. Comparing the backbone dynamics with other RRs, we propose that backbone flexibility is critical to the RR phosphorylation. Besides the limited flexibility, ETR1-RD has a unique γ loop conformation of opposite orientation, which makes ETR1-RD unfavorable for phosphorylation. These two features explain why ETR1-RD cannot be phosphorylated and is classified as an atypical type RR. As a control, phosphorylation of the ETR1-RD was also impaired when the sequence was swapped to the fragment of the bacterial typical type RR, CheY. Here, we suggest a molecule insight that the ETR1-RD already exists as an active formation and executes its function through binding with the downstream factors without phosphorylation. Public Library of Science 2016-08-03 /pmc/articles/PMC4972365/ /pubmed/27486797 http://dx.doi.org/10.1371/journal.pone.0160598 Text en © 2016 Hung et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Hung, Yi-Lin Jiang, Ingjye Lee, Yi-Zong Wen, Chi-Kuang Sue, Shih-Che NMR Study Reveals the Receiver Domain of Arabidopsis ETHYLENE RESPONSE1 Ethylene Receptor as an Atypical Type Response Regulator |
title | NMR Study Reveals the Receiver Domain of Arabidopsis ETHYLENE RESPONSE1 Ethylene Receptor as an Atypical Type Response Regulator |
title_full | NMR Study Reveals the Receiver Domain of Arabidopsis ETHYLENE RESPONSE1 Ethylene Receptor as an Atypical Type Response Regulator |
title_fullStr | NMR Study Reveals the Receiver Domain of Arabidopsis ETHYLENE RESPONSE1 Ethylene Receptor as an Atypical Type Response Regulator |
title_full_unstemmed | NMR Study Reveals the Receiver Domain of Arabidopsis ETHYLENE RESPONSE1 Ethylene Receptor as an Atypical Type Response Regulator |
title_short | NMR Study Reveals the Receiver Domain of Arabidopsis ETHYLENE RESPONSE1 Ethylene Receptor as an Atypical Type Response Regulator |
title_sort | nmr study reveals the receiver domain of arabidopsis ethylene response1 ethylene receptor as an atypical type response regulator |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4972365/ https://www.ncbi.nlm.nih.gov/pubmed/27486797 http://dx.doi.org/10.1371/journal.pone.0160598 |
work_keys_str_mv | AT hungyilin nmrstudyrevealsthereceiverdomainofarabidopsisethyleneresponse1ethylenereceptorasanatypicaltyperesponseregulator AT jiangingjye nmrstudyrevealsthereceiverdomainofarabidopsisethyleneresponse1ethylenereceptorasanatypicaltyperesponseregulator AT leeyizong nmrstudyrevealsthereceiverdomainofarabidopsisethyleneresponse1ethylenereceptorasanatypicaltyperesponseregulator AT wenchikuang nmrstudyrevealsthereceiverdomainofarabidopsisethyleneresponse1ethylenereceptorasanatypicaltyperesponseregulator AT sueshihche nmrstudyrevealsthereceiverdomainofarabidopsisethyleneresponse1ethylenereceptorasanatypicaltyperesponseregulator |