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A Novel Role for Banana MaASR in the Regulation of Flowering Time in Transgenic Arabidopsis

The abscisic acid (ABA)-, stress-, and ripening-induced (ASR) protein is a plant-specific hydrophilic transcriptional factor involved in fruit ripening and the abiotic stress response. To date, there have been no studies on the role of ASR genes in delayed flowering time. Here, we found that the ASR...

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Autores principales: Sun, Peiguang, Miao, Hongxia, Yu, Xiaomeng, Jia, Caihong, Liu, Juhua, Zhang, Jianbin, Wang, Jingyi, Wang, Zhuo, Wang, Anbang, Xu, Biyu, Jin, Zhiqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4972433/
https://www.ncbi.nlm.nih.gov/pubmed/27486844
http://dx.doi.org/10.1371/journal.pone.0160690
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author Sun, Peiguang
Miao, Hongxia
Yu, Xiaomeng
Jia, Caihong
Liu, Juhua
Zhang, Jianbin
Wang, Jingyi
Wang, Zhuo
Wang, Anbang
Xu, Biyu
Jin, Zhiqiang
author_facet Sun, Peiguang
Miao, Hongxia
Yu, Xiaomeng
Jia, Caihong
Liu, Juhua
Zhang, Jianbin
Wang, Jingyi
Wang, Zhuo
Wang, Anbang
Xu, Biyu
Jin, Zhiqiang
author_sort Sun, Peiguang
collection PubMed
description The abscisic acid (ABA)-, stress-, and ripening-induced (ASR) protein is a plant-specific hydrophilic transcriptional factor involved in fruit ripening and the abiotic stress response. To date, there have been no studies on the role of ASR genes in delayed flowering time. Here, we found that the ASR from banana, designated as MaASR, was preferentially expressed in the banana female flowers from the eighth, fourth, and first cluster of the inflorescence. MaASR transgenic lines (L14 and L38) had a clear delayed-flowering phenotype. The number of rosette leaves, sepals, and pedicel trichomes in L14 and L38 was greater than in the wild type (WT) under long day (LD) conditions. The period of buds, mid-flowers, and full bloom of L14 and L38 appeared later than the WT. cDNA microarray and quantitative real-time PCR (qRT-PCR) analyses revealed that overexpression of MaASR delays flowering through reduced expression of several genes, including photoperiod pathway genes, vernalization pathway genes, gibberellic acid pathway genes, and floral integrator genes, under short days (SD) for 28 d (from vegetative to reproductive transition stage); however, the expression of the autonomous pathway genes was not affected. This study provides the first evidence of a role for ASR genes in delayed flowering time in plants.
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spelling pubmed-49724332016-08-18 A Novel Role for Banana MaASR in the Regulation of Flowering Time in Transgenic Arabidopsis Sun, Peiguang Miao, Hongxia Yu, Xiaomeng Jia, Caihong Liu, Juhua Zhang, Jianbin Wang, Jingyi Wang, Zhuo Wang, Anbang Xu, Biyu Jin, Zhiqiang PLoS One Research Article The abscisic acid (ABA)-, stress-, and ripening-induced (ASR) protein is a plant-specific hydrophilic transcriptional factor involved in fruit ripening and the abiotic stress response. To date, there have been no studies on the role of ASR genes in delayed flowering time. Here, we found that the ASR from banana, designated as MaASR, was preferentially expressed in the banana female flowers from the eighth, fourth, and first cluster of the inflorescence. MaASR transgenic lines (L14 and L38) had a clear delayed-flowering phenotype. The number of rosette leaves, sepals, and pedicel trichomes in L14 and L38 was greater than in the wild type (WT) under long day (LD) conditions. The period of buds, mid-flowers, and full bloom of L14 and L38 appeared later than the WT. cDNA microarray and quantitative real-time PCR (qRT-PCR) analyses revealed that overexpression of MaASR delays flowering through reduced expression of several genes, including photoperiod pathway genes, vernalization pathway genes, gibberellic acid pathway genes, and floral integrator genes, under short days (SD) for 28 d (from vegetative to reproductive transition stage); however, the expression of the autonomous pathway genes was not affected. This study provides the first evidence of a role for ASR genes in delayed flowering time in plants. Public Library of Science 2016-08-03 /pmc/articles/PMC4972433/ /pubmed/27486844 http://dx.doi.org/10.1371/journal.pone.0160690 Text en © 2016 Sun et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Sun, Peiguang
Miao, Hongxia
Yu, Xiaomeng
Jia, Caihong
Liu, Juhua
Zhang, Jianbin
Wang, Jingyi
Wang, Zhuo
Wang, Anbang
Xu, Biyu
Jin, Zhiqiang
A Novel Role for Banana MaASR in the Regulation of Flowering Time in Transgenic Arabidopsis
title A Novel Role for Banana MaASR in the Regulation of Flowering Time in Transgenic Arabidopsis
title_full A Novel Role for Banana MaASR in the Regulation of Flowering Time in Transgenic Arabidopsis
title_fullStr A Novel Role for Banana MaASR in the Regulation of Flowering Time in Transgenic Arabidopsis
title_full_unstemmed A Novel Role for Banana MaASR in the Regulation of Flowering Time in Transgenic Arabidopsis
title_short A Novel Role for Banana MaASR in the Regulation of Flowering Time in Transgenic Arabidopsis
title_sort novel role for banana maasr in the regulation of flowering time in transgenic arabidopsis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4972433/
https://www.ncbi.nlm.nih.gov/pubmed/27486844
http://dx.doi.org/10.1371/journal.pone.0160690
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