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RoboDiff: combining a sample changer and goniometer for highly automated macromolecular crystallography experiments

Automation of the mounting of cryocooled samples is now a feature of the majority of beamlines dedicated to macromolecular crystallography (MX). Robotic sample changers have been developed over many years, with the latest designs increasing capacity, reliability and speed. Here, the development of a...

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Autores principales: Nurizzo, Didier, Bowler, Matthew W., Caserotto, Hugo, Dobias, Fabien, Giraud, Thierry, Surr, John, Guichard, Nicolas, Papp, Gergely, Guijarro, Matias, Mueller-Dieckmann, Christoph, Flot, David, McSweeney, Sean, Cipriani, Florent, Theveneau, Pascal, Leonard, Gordon A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4973212/
https://www.ncbi.nlm.nih.gov/pubmed/27487827
http://dx.doi.org/10.1107/S205979831601158X
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author Nurizzo, Didier
Bowler, Matthew W.
Caserotto, Hugo
Dobias, Fabien
Giraud, Thierry
Surr, John
Guichard, Nicolas
Papp, Gergely
Guijarro, Matias
Mueller-Dieckmann, Christoph
Flot, David
McSweeney, Sean
Cipriani, Florent
Theveneau, Pascal
Leonard, Gordon A.
author_facet Nurizzo, Didier
Bowler, Matthew W.
Caserotto, Hugo
Dobias, Fabien
Giraud, Thierry
Surr, John
Guichard, Nicolas
Papp, Gergely
Guijarro, Matias
Mueller-Dieckmann, Christoph
Flot, David
McSweeney, Sean
Cipriani, Florent
Theveneau, Pascal
Leonard, Gordon A.
author_sort Nurizzo, Didier
collection PubMed
description Automation of the mounting of cryocooled samples is now a feature of the majority of beamlines dedicated to macromolecular crystallography (MX). Robotic sample changers have been developed over many years, with the latest designs increasing capacity, reliability and speed. Here, the development of a new sample changer deployed at the ESRF beamline MASSIF-1 (ID30A-1), based on an industrial six-axis robot, is described. The device, named RoboDiff, includes a high-capacity dewar, acts as both a sample changer and a high-accuracy goniometer, and has been designed for completely unattended sample mounting and diffraction data collection. This aim has been achieved using a high level of diagnostics at all steps of the process from mounting and characterization to data collection. The RoboDiff has been in service on the fully automated endstation MASSIF-1 at the ESRF since September 2014 and, at the time of writing, has processed more than 20 000 samples completely automatically.
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spelling pubmed-49732122016-08-17 RoboDiff: combining a sample changer and goniometer for highly automated macromolecular crystallography experiments Nurizzo, Didier Bowler, Matthew W. Caserotto, Hugo Dobias, Fabien Giraud, Thierry Surr, John Guichard, Nicolas Papp, Gergely Guijarro, Matias Mueller-Dieckmann, Christoph Flot, David McSweeney, Sean Cipriani, Florent Theveneau, Pascal Leonard, Gordon A. Acta Crystallogr D Struct Biol Research Papers Automation of the mounting of cryocooled samples is now a feature of the majority of beamlines dedicated to macromolecular crystallography (MX). Robotic sample changers have been developed over many years, with the latest designs increasing capacity, reliability and speed. Here, the development of a new sample changer deployed at the ESRF beamline MASSIF-1 (ID30A-1), based on an industrial six-axis robot, is described. The device, named RoboDiff, includes a high-capacity dewar, acts as both a sample changer and a high-accuracy goniometer, and has been designed for completely unattended sample mounting and diffraction data collection. This aim has been achieved using a high level of diagnostics at all steps of the process from mounting and characterization to data collection. The RoboDiff has been in service on the fully automated endstation MASSIF-1 at the ESRF since September 2014 and, at the time of writing, has processed more than 20 000 samples completely automatically. International Union of Crystallography 2016-07-27 /pmc/articles/PMC4973212/ /pubmed/27487827 http://dx.doi.org/10.1107/S205979831601158X Text en © Nurizzo et al. 2016 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Research Papers
Nurizzo, Didier
Bowler, Matthew W.
Caserotto, Hugo
Dobias, Fabien
Giraud, Thierry
Surr, John
Guichard, Nicolas
Papp, Gergely
Guijarro, Matias
Mueller-Dieckmann, Christoph
Flot, David
McSweeney, Sean
Cipriani, Florent
Theveneau, Pascal
Leonard, Gordon A.
RoboDiff: combining a sample changer and goniometer for highly automated macromolecular crystallography experiments
title RoboDiff: combining a sample changer and goniometer for highly automated macromolecular crystallography experiments
title_full RoboDiff: combining a sample changer and goniometer for highly automated macromolecular crystallography experiments
title_fullStr RoboDiff: combining a sample changer and goniometer for highly automated macromolecular crystallography experiments
title_full_unstemmed RoboDiff: combining a sample changer and goniometer for highly automated macromolecular crystallography experiments
title_short RoboDiff: combining a sample changer and goniometer for highly automated macromolecular crystallography experiments
title_sort robodiff: combining a sample changer and goniometer for highly automated macromolecular crystallography experiments
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4973212/
https://www.ncbi.nlm.nih.gov/pubmed/27487827
http://dx.doi.org/10.1107/S205979831601158X
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