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Loss of l(3)mbt leads to acquisition of the ping-pong cycle in Drosophila ovarian somatic cells
In Drosophila germ cells, PIWI-interacting RNAs (piRNAs) are amplified through a PIWI slicer-dependent feed-forward loop termed the ping-pong cycle, yielding secondary piRNAs. However, the detailed mechanism remains poorly understood, largely because an ex vivo model system amenable to biochemical a...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4973291/ https://www.ncbi.nlm.nih.gov/pubmed/27474440 http://dx.doi.org/10.1101/gad.283929.116 |
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author | Sumiyoshi, Tetsutaro Sato, Kaoru Yamamoto, Hitomi Iwasaki, Yuka W. Siomi, Haruhiko Siomi, Mikiko C. |
author_facet | Sumiyoshi, Tetsutaro Sato, Kaoru Yamamoto, Hitomi Iwasaki, Yuka W. Siomi, Haruhiko Siomi, Mikiko C. |
author_sort | Sumiyoshi, Tetsutaro |
collection | PubMed |
description | In Drosophila germ cells, PIWI-interacting RNAs (piRNAs) are amplified through a PIWI slicer-dependent feed-forward loop termed the ping-pong cycle, yielding secondary piRNAs. However, the detailed mechanism remains poorly understood, largely because an ex vivo model system amenable to biochemical analyses has not been available. Here, we show that CRISPR-mediated loss of function of lethal (3) malignant brain tumor [l(3)mbt] leads to ectopic activation of the germ-specific ping-pong cycle in ovarian somatic cells. Perinuclear foci resembling nuage, the ping-pong center, appeared following l(3)mbt mutation. This activation of the ping-pong machinery in cultured cells will greatly facilitate elucidation of the mechanism underlying secondary piRNA biogenesis in Drosophila. |
format | Online Article Text |
id | pubmed-4973291 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-49732912016-08-25 Loss of l(3)mbt leads to acquisition of the ping-pong cycle in Drosophila ovarian somatic cells Sumiyoshi, Tetsutaro Sato, Kaoru Yamamoto, Hitomi Iwasaki, Yuka W. Siomi, Haruhiko Siomi, Mikiko C. Genes Dev Research Communication In Drosophila germ cells, PIWI-interacting RNAs (piRNAs) are amplified through a PIWI slicer-dependent feed-forward loop termed the ping-pong cycle, yielding secondary piRNAs. However, the detailed mechanism remains poorly understood, largely because an ex vivo model system amenable to biochemical analyses has not been available. Here, we show that CRISPR-mediated loss of function of lethal (3) malignant brain tumor [l(3)mbt] leads to ectopic activation of the germ-specific ping-pong cycle in ovarian somatic cells. Perinuclear foci resembling nuage, the ping-pong center, appeared following l(3)mbt mutation. This activation of the ping-pong machinery in cultured cells will greatly facilitate elucidation of the mechanism underlying secondary piRNA biogenesis in Drosophila. Cold Spring Harbor Laboratory Press 2016-07-15 /pmc/articles/PMC4973291/ /pubmed/27474440 http://dx.doi.org/10.1101/gad.283929.116 Text en © 2016 Sumiyoshi et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by/4.0/ This article, published in Genes & Development, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Research Communication Sumiyoshi, Tetsutaro Sato, Kaoru Yamamoto, Hitomi Iwasaki, Yuka W. Siomi, Haruhiko Siomi, Mikiko C. Loss of l(3)mbt leads to acquisition of the ping-pong cycle in Drosophila ovarian somatic cells |
title | Loss of l(3)mbt leads to acquisition of the ping-pong cycle in Drosophila ovarian somatic cells |
title_full | Loss of l(3)mbt leads to acquisition of the ping-pong cycle in Drosophila ovarian somatic cells |
title_fullStr | Loss of l(3)mbt leads to acquisition of the ping-pong cycle in Drosophila ovarian somatic cells |
title_full_unstemmed | Loss of l(3)mbt leads to acquisition of the ping-pong cycle in Drosophila ovarian somatic cells |
title_short | Loss of l(3)mbt leads to acquisition of the ping-pong cycle in Drosophila ovarian somatic cells |
title_sort | loss of l(3)mbt leads to acquisition of the ping-pong cycle in drosophila ovarian somatic cells |
topic | Research Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4973291/ https://www.ncbi.nlm.nih.gov/pubmed/27474440 http://dx.doi.org/10.1101/gad.283929.116 |
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