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Dissecting the role of Wnt signaling and its interactions with FGF signaling during midbrain neurogenesis
Interactions between FGF and Wnt/ bcat signaling control development of the midbrain. The nature of this interaction and how these regulate patterning, growth and differentiation is less clear, as it has not been possible to temporally dissect the effects of one pathway relative to the other. We hav...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4973611/ https://www.ncbi.nlm.nih.gov/pubmed/27606327 http://dx.doi.org/10.1080/23262133.2015.1057313 |
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author | Dyer, Carlene Blanc, Eric Stanley, Rob J Knight, Robert D |
author_facet | Dyer, Carlene Blanc, Eric Stanley, Rob J Knight, Robert D |
author_sort | Dyer, Carlene |
collection | PubMed |
description | Interactions between FGF and Wnt/ bcat signaling control development of the midbrain. The nature of this interaction and how these regulate patterning, growth and differentiation is less clear, as it has not been possible to temporally dissect the effects of one pathway relative to the other. We have employed pharmacological and genetic tools to probe the temporal and spatial roles of FGF and Wnt in controlling the specification of early midbrain neurons. We identify a β-catenin (bcat) independent role for GSK-3 in modulating FGF activity and hence neuronal patterning. This function is complicated by an overlap with bcat-dependent regulation of FGF signaling, through the regulation of sprouty4. Additionally we reveal how attenuation of Axin protein function can promote fluctuating levels of bcat activity that are dependent on FGF activity. This highlights the complex nature of the interactions between FGF and Wnt/ bcat and reveals that they act at multiple levels to control each others activity in the midbrain. |
format | Online Article Text |
id | pubmed-4973611 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-49736112016-09-21 Dissecting the role of Wnt signaling and its interactions with FGF signaling during midbrain neurogenesis Dyer, Carlene Blanc, Eric Stanley, Rob J Knight, Robert D Neurogenesis (Austin) Research Paper Interactions between FGF and Wnt/ bcat signaling control development of the midbrain. The nature of this interaction and how these regulate patterning, growth and differentiation is less clear, as it has not been possible to temporally dissect the effects of one pathway relative to the other. We have employed pharmacological and genetic tools to probe the temporal and spatial roles of FGF and Wnt in controlling the specification of early midbrain neurons. We identify a β-catenin (bcat) independent role for GSK-3 in modulating FGF activity and hence neuronal patterning. This function is complicated by an overlap with bcat-dependent regulation of FGF signaling, through the regulation of sprouty4. Additionally we reveal how attenuation of Axin protein function can promote fluctuating levels of bcat activity that are dependent on FGF activity. This highlights the complex nature of the interactions between FGF and Wnt/ bcat and reveals that they act at multiple levels to control each others activity in the midbrain. Taylor & Francis 2015-09-21 /pmc/articles/PMC4973611/ /pubmed/27606327 http://dx.doi.org/10.1080/23262133.2015.1057313 Text en © 2015 The Author(s). Published with license by Taylor & Francis http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted. |
spellingShingle | Research Paper Dyer, Carlene Blanc, Eric Stanley, Rob J Knight, Robert D Dissecting the role of Wnt signaling and its interactions with FGF signaling during midbrain neurogenesis |
title | Dissecting the role of Wnt signaling and its interactions with FGF signaling during midbrain neurogenesis |
title_full | Dissecting the role of Wnt signaling and its interactions with FGF signaling during midbrain neurogenesis |
title_fullStr | Dissecting the role of Wnt signaling and its interactions with FGF signaling during midbrain neurogenesis |
title_full_unstemmed | Dissecting the role of Wnt signaling and its interactions with FGF signaling during midbrain neurogenesis |
title_short | Dissecting the role of Wnt signaling and its interactions with FGF signaling during midbrain neurogenesis |
title_sort | dissecting the role of wnt signaling and its interactions with fgf signaling during midbrain neurogenesis |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4973611/ https://www.ncbi.nlm.nih.gov/pubmed/27606327 http://dx.doi.org/10.1080/23262133.2015.1057313 |
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