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Inhibition of prostate cancer growth using doxorubicin assisted by ultrasound-targeted nanobubble destruction

Ultrasound (US)-targeted microbubble destruction has been widely used as an effective drug-delivery system. However, nanobubbles (NBs) have better stability and stronger penetration than microbubbles, and drug delivery assisted by US-targeted NB destruction (UTND) still needs to be investigated. Our...

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Autores principales: Fan, Xiaozhou, Wang, Luofu, Guo, Yanli, Xiong, Xingyu, Zhu, Lianhua, Fang, Kejing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4973723/
https://www.ncbi.nlm.nih.gov/pubmed/27536100
http://dx.doi.org/10.2147/IJN.S111808
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author Fan, Xiaozhou
Wang, Luofu
Guo, Yanli
Xiong, Xingyu
Zhu, Lianhua
Fang, Kejing
author_facet Fan, Xiaozhou
Wang, Luofu
Guo, Yanli
Xiong, Xingyu
Zhu, Lianhua
Fang, Kejing
author_sort Fan, Xiaozhou
collection PubMed
description Ultrasound (US)-targeted microbubble destruction has been widely used as an effective drug-delivery system. However, nanobubbles (NBs) have better stability and stronger penetration than microbubbles, and drug delivery assisted by US-targeted NB destruction (UTND) still needs to be investigated. Our aim was to investigate the effect of doxorubicin (DOX) on the inhibition of prostate cancer growth under UTND. Contrast-enhanced US imaging of transplanted PC3 prostate cancer in mice showed that under a combination of 1 W/cm(2) US power and a 100 Hz intermittent pulse with a “5 seconds on, 5 seconds off” mode, NBs with an average size of (485.7±33) nm were effectively destroyed within 15 minutes in the tumor location. PC3 cells and 20 tumor-bearing mice were divided into four groups: a DOX group, a DOX + NB group, a DOX + US group, and a DOX + NB + US group. The cell growth-inhibition rate and DOX concentration of xenografts in the DOX + NB + US group were highest. Based on another control group and these four groups, another 25 tumor-bearing mice were used to observe the treatment effect of nine DOX injections under UTND. The xenografts in the DOX + NB + US group decreased more obviously and had more cellular apoptosis than other groups. Finally, electron microscopy was used to estimate the cavitation effect of NBs under US irradiation in the control group, NB group, US group, and NB + US group. The results of scanning electron microscopy showed that PC3 cells in the DOX + NB + US group had more holes and significantly increased cell-surface folds. Meanwhile, transmission electric microscopy confirmed that more lanthanum nitrate particles entered the parenchymal cells in xenografts in the NB + US group compared with the other groups. This study suggested that UTND technology could be an effective method to promote drugs to function in US-irradiated sites, and the underlying mechanism may be associated with a cavitation effect.
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spelling pubmed-49737232016-08-17 Inhibition of prostate cancer growth using doxorubicin assisted by ultrasound-targeted nanobubble destruction Fan, Xiaozhou Wang, Luofu Guo, Yanli Xiong, Xingyu Zhu, Lianhua Fang, Kejing Int J Nanomedicine Original Research Ultrasound (US)-targeted microbubble destruction has been widely used as an effective drug-delivery system. However, nanobubbles (NBs) have better stability and stronger penetration than microbubbles, and drug delivery assisted by US-targeted NB destruction (UTND) still needs to be investigated. Our aim was to investigate the effect of doxorubicin (DOX) on the inhibition of prostate cancer growth under UTND. Contrast-enhanced US imaging of transplanted PC3 prostate cancer in mice showed that under a combination of 1 W/cm(2) US power and a 100 Hz intermittent pulse with a “5 seconds on, 5 seconds off” mode, NBs with an average size of (485.7±33) nm were effectively destroyed within 15 minutes in the tumor location. PC3 cells and 20 tumor-bearing mice were divided into four groups: a DOX group, a DOX + NB group, a DOX + US group, and a DOX + NB + US group. The cell growth-inhibition rate and DOX concentration of xenografts in the DOX + NB + US group were highest. Based on another control group and these four groups, another 25 tumor-bearing mice were used to observe the treatment effect of nine DOX injections under UTND. The xenografts in the DOX + NB + US group decreased more obviously and had more cellular apoptosis than other groups. Finally, electron microscopy was used to estimate the cavitation effect of NBs under US irradiation in the control group, NB group, US group, and NB + US group. The results of scanning electron microscopy showed that PC3 cells in the DOX + NB + US group had more holes and significantly increased cell-surface folds. Meanwhile, transmission electric microscopy confirmed that more lanthanum nitrate particles entered the parenchymal cells in xenografts in the NB + US group compared with the other groups. This study suggested that UTND technology could be an effective method to promote drugs to function in US-irradiated sites, and the underlying mechanism may be associated with a cavitation effect. Dove Medical Press 2016-07-29 /pmc/articles/PMC4973723/ /pubmed/27536100 http://dx.doi.org/10.2147/IJN.S111808 Text en © 2016 Fan et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Fan, Xiaozhou
Wang, Luofu
Guo, Yanli
Xiong, Xingyu
Zhu, Lianhua
Fang, Kejing
Inhibition of prostate cancer growth using doxorubicin assisted by ultrasound-targeted nanobubble destruction
title Inhibition of prostate cancer growth using doxorubicin assisted by ultrasound-targeted nanobubble destruction
title_full Inhibition of prostate cancer growth using doxorubicin assisted by ultrasound-targeted nanobubble destruction
title_fullStr Inhibition of prostate cancer growth using doxorubicin assisted by ultrasound-targeted nanobubble destruction
title_full_unstemmed Inhibition of prostate cancer growth using doxorubicin assisted by ultrasound-targeted nanobubble destruction
title_short Inhibition of prostate cancer growth using doxorubicin assisted by ultrasound-targeted nanobubble destruction
title_sort inhibition of prostate cancer growth using doxorubicin assisted by ultrasound-targeted nanobubble destruction
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4973723/
https://www.ncbi.nlm.nih.gov/pubmed/27536100
http://dx.doi.org/10.2147/IJN.S111808
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