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Protein N-terminal acetylation is required for embryogenesis in Arabidopsis

Early embryonic development generates precursors of all major cell types in Arabidopsis. Among these precursors, the hypophysis divides asymmetrically to form the progenitors of the quiescent center and columella stem cells. A great deal has been learnt about the mechanisms that control the asymmetr...

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Autores principales: Feng, Jinlin, Li, Ruiqi, Yu, Junya, Ma, Shuangshuang, Wu, Chunyan, Li, Yan, Cao, Ying, Ma, Ligeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4973746/
https://www.ncbi.nlm.nih.gov/pubmed/27385766
http://dx.doi.org/10.1093/jxb/erw257
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author Feng, Jinlin
Li, Ruiqi
Yu, Junya
Ma, Shuangshuang
Wu, Chunyan
Li, Yan
Cao, Ying
Ma, Ligeng
author_facet Feng, Jinlin
Li, Ruiqi
Yu, Junya
Ma, Shuangshuang
Wu, Chunyan
Li, Yan
Cao, Ying
Ma, Ligeng
author_sort Feng, Jinlin
collection PubMed
description Early embryonic development generates precursors of all major cell types in Arabidopsis. Among these precursors, the hypophysis divides asymmetrically to form the progenitors of the quiescent center and columella stem cells. A great deal has been learnt about the mechanisms that control the asymmetric division of the hypophysis and embryogenesis at the transcriptional level; however, no evidence of regulation at the co- or post-translational level has been reported. Here, we show that mutation of the catalytic subunit (Naa10) or auxiliary subunit (Naa15) of NatA, an N-terminal acetyltransferase that catalyzes protein N-terminal acetylation, produces an embryo-lethal phenotype. In addition, Naa10 and Naa15 were found to interact physically in planta. Further analysis revealed that the observed embryonic patterning defects started at the early globular stage and that the asymmetric division of the hypophysis was irregular; thus, no quiescent center progenitor cells were generated in naa10 and naa15 embryos. We further observed that the polar distributions of auxin and its efflux carrier PIN1 were disturbed in naa10 embryos. Our results suggest that NatA is required for asymmetric division of the hypophysis and early embryonic patterning in Arabidopsis, and provides a link between protein N-terminal acetylation and embryogenesis in plants.
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spelling pubmed-49737462016-08-05 Protein N-terminal acetylation is required for embryogenesis in Arabidopsis Feng, Jinlin Li, Ruiqi Yu, Junya Ma, Shuangshuang Wu, Chunyan Li, Yan Cao, Ying Ma, Ligeng J Exp Bot Research Paper Early embryonic development generates precursors of all major cell types in Arabidopsis. Among these precursors, the hypophysis divides asymmetrically to form the progenitors of the quiescent center and columella stem cells. A great deal has been learnt about the mechanisms that control the asymmetric division of the hypophysis and embryogenesis at the transcriptional level; however, no evidence of regulation at the co- or post-translational level has been reported. Here, we show that mutation of the catalytic subunit (Naa10) or auxiliary subunit (Naa15) of NatA, an N-terminal acetyltransferase that catalyzes protein N-terminal acetylation, produces an embryo-lethal phenotype. In addition, Naa10 and Naa15 were found to interact physically in planta. Further analysis revealed that the observed embryonic patterning defects started at the early globular stage and that the asymmetric division of the hypophysis was irregular; thus, no quiescent center progenitor cells were generated in naa10 and naa15 embryos. We further observed that the polar distributions of auxin and its efflux carrier PIN1 were disturbed in naa10 embryos. Our results suggest that NatA is required for asymmetric division of the hypophysis and early embryonic patterning in Arabidopsis, and provides a link between protein N-terminal acetylation and embryogenesis in plants. Oxford University Press 2016-08 2016-07-06 /pmc/articles/PMC4973746/ /pubmed/27385766 http://dx.doi.org/10.1093/jxb/erw257 Text en © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Feng, Jinlin
Li, Ruiqi
Yu, Junya
Ma, Shuangshuang
Wu, Chunyan
Li, Yan
Cao, Ying
Ma, Ligeng
Protein N-terminal acetylation is required for embryogenesis in Arabidopsis
title Protein N-terminal acetylation is required for embryogenesis in Arabidopsis
title_full Protein N-terminal acetylation is required for embryogenesis in Arabidopsis
title_fullStr Protein N-terminal acetylation is required for embryogenesis in Arabidopsis
title_full_unstemmed Protein N-terminal acetylation is required for embryogenesis in Arabidopsis
title_short Protein N-terminal acetylation is required for embryogenesis in Arabidopsis
title_sort protein n-terminal acetylation is required for embryogenesis in arabidopsis
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4973746/
https://www.ncbi.nlm.nih.gov/pubmed/27385766
http://dx.doi.org/10.1093/jxb/erw257
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