Expression Pattern of Mouse Vasa Homologue (MVH) in the Ovaries of C57BL/6 Female Mice

BACKGROUND: Vasa (a DEAD-box helicase, also known as Ddx4) is an ATP-dependent RNA helicase highly conserved among all animals. Research on the presence and function of DDX4 in female mammals is limited. To gain greater insight into its distribution and role in female mice, we detected the expressio...

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Detalles Bibliográficos
Autores principales: Song, Kunkun, Ma, Wenwen, Huang, Cong, Ding, Jiahui, Cui, Dandan, Zhang, Mingmin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scientific Literature, Inc. 2016
Materias:
Animal Study
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4973802/
https://www.ncbi.nlm.nih.gov/pubmed/27460133
http://dx.doi.org/10.12659/MSM.899830
Descripción
Sumario:BACKGROUND: Vasa (a DEAD-box helicase, also known as Ddx4) is an ATP-dependent RNA helicase highly conserved among all animals. Research on the presence and function of DDX4 in female mammals is limited. To gain greater insight into its distribution and role in female mice, we detected the expression of DDX4 protein in the ovaries and analyzed its expression pattern. MATERIAL/METHODS: MVH was detected in the cytoplasm of oocytes in all non-apoptotic follicles. RESULTS: In the present study, we found that higher expression levels of ~55–60 kDa MVH isoform in the ovaries were followed by the accumulations of preovulatory follicles. CONCLUSIONS: Higher levels of MVH protein in the ovaries might prepare oocytes for the competence to resume meiosis.

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