Expression Pattern of Mouse Vasa Homologue (MVH) in the Ovaries of C57BL/6 Female Mice

BACKGROUND: Vasa (a DEAD-box helicase, also known as Ddx4) is an ATP-dependent RNA helicase highly conserved among all animals. Research on the presence and function of DDX4 in female mammals is limited. To gain greater insight into its distribution and role in female mice, we detected the expressio...

Descripción completa

Detalles Bibliográficos
Autores principales: Song, Kunkun, Ma, Wenwen, Huang, Cong, Ding, Jiahui, Cui, Dandan, Zhang, Mingmin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scientific Literature, Inc. 2016
Materias:
Animal Study
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4973802/
https://www.ncbi.nlm.nih.gov/pubmed/27460133
http://dx.doi.org/10.12659/MSM.899830
_version_ 1782446453523742720
author Song, Kunkun
Ma, Wenwen
Huang, Cong
Ding, Jiahui
Cui, Dandan
Zhang, Mingmin
author_facet Song, Kunkun
Ma, Wenwen
Huang, Cong
Ding, Jiahui
Cui, Dandan
Zhang, Mingmin
author_sort Song, Kunkun
collection PubMed
description BACKGROUND: Vasa (a DEAD-box helicase, also known as Ddx4) is an ATP-dependent RNA helicase highly conserved among all animals. Research on the presence and function of DDX4 in female mammals is limited. To gain greater insight into its distribution and role in female mice, we detected the expression of DDX4 protein in the ovaries and analyzed its expression pattern. MATERIAL/METHODS: MVH was detected in the cytoplasm of oocytes in all non-apoptotic follicles. RESULTS: In the present study, we found that higher expression levels of ~55–60 kDa MVH isoform in the ovaries were followed by the accumulations of preovulatory follicles. CONCLUSIONS: Higher levels of MVH protein in the ovaries might prepare oocytes for the competence to resume meiosis.
format Online
Article
Text
id pubmed-4973802
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher International Scientific Literature, Inc.
record_format MEDLINE/PubMed
spelling pubmed-49738022016-08-19 Expression Pattern of Mouse Vasa Homologue (MVH) in the Ovaries of C57BL/6 Female Mice Song, Kunkun Ma, Wenwen Huang, Cong Ding, Jiahui Cui, Dandan Zhang, Mingmin Med Sci Monit Animal Study BACKGROUND: Vasa (a DEAD-box helicase, also known as Ddx4) is an ATP-dependent RNA helicase highly conserved among all animals. Research on the presence and function of DDX4 in female mammals is limited. To gain greater insight into its distribution and role in female mice, we detected the expression of DDX4 protein in the ovaries and analyzed its expression pattern. MATERIAL/METHODS: MVH was detected in the cytoplasm of oocytes in all non-apoptotic follicles. RESULTS: In the present study, we found that higher expression levels of ~55–60 kDa MVH isoform in the ovaries were followed by the accumulations of preovulatory follicles. CONCLUSIONS: Higher levels of MVH protein in the ovaries might prepare oocytes for the competence to resume meiosis. International Scientific Literature, Inc. 2016-07-27 /pmc/articles/PMC4973802/ /pubmed/27460133 http://dx.doi.org/10.12659/MSM.899830 Text en © Med Sci Monit, 2016 This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
spellingShingle Animal Study
Song, Kunkun
Ma, Wenwen
Huang, Cong
Ding, Jiahui
Cui, Dandan
Zhang, Mingmin
Expression Pattern of Mouse Vasa Homologue (MVH) in the Ovaries of C57BL/6 Female Mice
title Expression Pattern of Mouse Vasa Homologue (MVH) in the Ovaries of C57BL/6 Female Mice
title_full Expression Pattern of Mouse Vasa Homologue (MVH) in the Ovaries of C57BL/6 Female Mice
title_fullStr Expression Pattern of Mouse Vasa Homologue (MVH) in the Ovaries of C57BL/6 Female Mice
title_full_unstemmed Expression Pattern of Mouse Vasa Homologue (MVH) in the Ovaries of C57BL/6 Female Mice
title_short Expression Pattern of Mouse Vasa Homologue (MVH) in the Ovaries of C57BL/6 Female Mice
title_sort expression pattern of mouse vasa homologue (mvh) in the ovaries of c57bl/6 female mice
topic Animal Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4973802/
https://www.ncbi.nlm.nih.gov/pubmed/27460133
http://dx.doi.org/10.12659/MSM.899830
work_keys_str_mv AT songkunkun expressionpatternofmousevasahomologuemvhintheovariesofc57bl6femalemice
AT mawenwen expressionpatternofmousevasahomologuemvhintheovariesofc57bl6femalemice
AT huangcong expressionpatternofmousevasahomologuemvhintheovariesofc57bl6femalemice
AT dingjiahui expressionpatternofmousevasahomologuemvhintheovariesofc57bl6femalemice
AT cuidandan expressionpatternofmousevasahomologuemvhintheovariesofc57bl6femalemice
AT zhangmingmin expressionpatternofmousevasahomologuemvhintheovariesofc57bl6femalemice

Ejemplares similares