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Metaproteomics and metabolomics analyses of chronically petroleum‐polluted sites reveal the importance of general anaerobic processes uncoupled with degradation

Crude oil is one of the most important natural assets for humankind, yet it is a major environmental pollutant, notably in marine environments. One of the largest crude oil polluted areas in the word is the semi‐enclosed Mediterranean Sea, in which the metabolic potential of indigenous microbial pop...

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Autores principales: Bargiela, Rafael, Herbst, Florian‐Alexander, Martínez‐Martínez, Mónica, Seifert, Jana, Rojo, David, Cappello, Simone, Genovese, María, Crisafi, Francesca, Denaro, Renata, Chernikova, Tatyana N., Barbas, Coral, von Bergen, Martin, Yakimov, Michail M., Ferrer, Manuel, Golyshin, Peter N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4973819/
https://www.ncbi.nlm.nih.gov/pubmed/26201687
http://dx.doi.org/10.1002/pmic.201400614
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author Bargiela, Rafael
Herbst, Florian‐Alexander
Martínez‐Martínez, Mónica
Seifert, Jana
Rojo, David
Cappello, Simone
Genovese, María
Crisafi, Francesca
Denaro, Renata
Chernikova, Tatyana N.
Barbas, Coral
von Bergen, Martin
Yakimov, Michail M.
Ferrer, Manuel
Golyshin, Peter N.
author_facet Bargiela, Rafael
Herbst, Florian‐Alexander
Martínez‐Martínez, Mónica
Seifert, Jana
Rojo, David
Cappello, Simone
Genovese, María
Crisafi, Francesca
Denaro, Renata
Chernikova, Tatyana N.
Barbas, Coral
von Bergen, Martin
Yakimov, Michail M.
Ferrer, Manuel
Golyshin, Peter N.
author_sort Bargiela, Rafael
collection PubMed
description Crude oil is one of the most important natural assets for humankind, yet it is a major environmental pollutant, notably in marine environments. One of the largest crude oil polluted areas in the word is the semi‐enclosed Mediterranean Sea, in which the metabolic potential of indigenous microbial populations towards the large‐scale chronic pollution is yet to be defined, particularly in anaerobic and micro‐aerophilic sites. Here, we provide an insight into the microbial metabolism in sediments from three chronically polluted marine sites along the coastline of Italy: the Priolo oil terminal/refinery site (near Siracuse, Sicily), harbour of Messina (Sicily) and shipwreck of MT Haven (near Genoa). Using shotgun metaproteomics and community metabolomics approaches, the presence of 651 microbial proteins and 4776 metabolite mass features have been detected in these three environments, revealing a high metabolic heterogeneity between the investigated sites. The proteomes displayed the prevalence of anaerobic metabolisms that were not directly related with petroleum biodegradation, indicating that in the absence of oxygen, biodegradation is significantly suppressed. This suppression was also suggested by examining the metabolome patterns. The proteome analysis further highlighted the metabolic coupling between methylotrophs and sulphate reducers in oxygen‐depleted petroleum‐polluted sediments.
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spelling pubmed-49738192016-08-17 Metaproteomics and metabolomics analyses of chronically petroleum‐polluted sites reveal the importance of general anaerobic processes uncoupled with degradation Bargiela, Rafael Herbst, Florian‐Alexander Martínez‐Martínez, Mónica Seifert, Jana Rojo, David Cappello, Simone Genovese, María Crisafi, Francesca Denaro, Renata Chernikova, Tatyana N. Barbas, Coral von Bergen, Martin Yakimov, Michail M. Ferrer, Manuel Golyshin, Peter N. Proteomics Research Articles Crude oil is one of the most important natural assets for humankind, yet it is a major environmental pollutant, notably in marine environments. One of the largest crude oil polluted areas in the word is the semi‐enclosed Mediterranean Sea, in which the metabolic potential of indigenous microbial populations towards the large‐scale chronic pollution is yet to be defined, particularly in anaerobic and micro‐aerophilic sites. Here, we provide an insight into the microbial metabolism in sediments from three chronically polluted marine sites along the coastline of Italy: the Priolo oil terminal/refinery site (near Siracuse, Sicily), harbour of Messina (Sicily) and shipwreck of MT Haven (near Genoa). Using shotgun metaproteomics and community metabolomics approaches, the presence of 651 microbial proteins and 4776 metabolite mass features have been detected in these three environments, revealing a high metabolic heterogeneity between the investigated sites. The proteomes displayed the prevalence of anaerobic metabolisms that were not directly related with petroleum biodegradation, indicating that in the absence of oxygen, biodegradation is significantly suppressed. This suppression was also suggested by examining the metabolome patterns. The proteome analysis further highlighted the metabolic coupling between methylotrophs and sulphate reducers in oxygen‐depleted petroleum‐polluted sediments. John Wiley and Sons Inc. 2015-08-27 2015-10 /pmc/articles/PMC4973819/ /pubmed/26201687 http://dx.doi.org/10.1002/pmic.201400614 Text en © 2015 The Authors. PROTEOMICS published by Wiley‐VCH Verlag GmbH & Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Bargiela, Rafael
Herbst, Florian‐Alexander
Martínez‐Martínez, Mónica
Seifert, Jana
Rojo, David
Cappello, Simone
Genovese, María
Crisafi, Francesca
Denaro, Renata
Chernikova, Tatyana N.
Barbas, Coral
von Bergen, Martin
Yakimov, Michail M.
Ferrer, Manuel
Golyshin, Peter N.
Metaproteomics and metabolomics analyses of chronically petroleum‐polluted sites reveal the importance of general anaerobic processes uncoupled with degradation
title Metaproteomics and metabolomics analyses of chronically petroleum‐polluted sites reveal the importance of general anaerobic processes uncoupled with degradation
title_full Metaproteomics and metabolomics analyses of chronically petroleum‐polluted sites reveal the importance of general anaerobic processes uncoupled with degradation
title_fullStr Metaproteomics and metabolomics analyses of chronically petroleum‐polluted sites reveal the importance of general anaerobic processes uncoupled with degradation
title_full_unstemmed Metaproteomics and metabolomics analyses of chronically petroleum‐polluted sites reveal the importance of general anaerobic processes uncoupled with degradation
title_short Metaproteomics and metabolomics analyses of chronically petroleum‐polluted sites reveal the importance of general anaerobic processes uncoupled with degradation
title_sort metaproteomics and metabolomics analyses of chronically petroleum‐polluted sites reveal the importance of general anaerobic processes uncoupled with degradation
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4973819/
https://www.ncbi.nlm.nih.gov/pubmed/26201687
http://dx.doi.org/10.1002/pmic.201400614
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