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High-rate, High Temperature Acetotrophic Methanogenesis Governed by a Three Population Consortium in Anaerobic Bioreactors

A combination of acetate oxidation and acetoclastic methanogenesis has been previously identified to enable high-rate methanogenesis at high temperatures (55 to 65°C), but this capability had not been linked to any key organisms. This study combined RNA–stable isotope probing on (13)C-labelled aceta...

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Autores principales: Ho, Dang, Jensen, Paul, Gutierrez-Zamora, Maria-Luisa, Beckmann, Sabrina, Manefield, Mike, Batstone, Damien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4973872/
https://www.ncbi.nlm.nih.gov/pubmed/27490246
http://dx.doi.org/10.1371/journal.pone.0159760
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author Ho, Dang
Jensen, Paul
Gutierrez-Zamora, Maria-Luisa
Beckmann, Sabrina
Manefield, Mike
Batstone, Damien
author_facet Ho, Dang
Jensen, Paul
Gutierrez-Zamora, Maria-Luisa
Beckmann, Sabrina
Manefield, Mike
Batstone, Damien
author_sort Ho, Dang
collection PubMed
description A combination of acetate oxidation and acetoclastic methanogenesis has been previously identified to enable high-rate methanogenesis at high temperatures (55 to 65°C), but this capability had not been linked to any key organisms. This study combined RNA–stable isotope probing on (13)C-labelled acetate and 16S amplicon sequencing to identify the active micro-organisms involved in high-rate methanogenesis. Active biomass was harvested from three bench-scale thermophilic bioreactors treating waste activated sludge at 55, 60 and 65°C, and fed with (13)-C labelled and (12)C-unlabelled acetate. Acetate uptake and cumulative methane production were determined and kinetic parameters were estimated using model-based analysis. Pyrosequencing performed on (13)C- enriched samples indicated that organisms accumulating labelled carbon were Coprothermobacter (all temperatures between 55 and 65°C), acetoclastic Methanosarcina (55 to 60°C) and hydrogenotrophic Methanothermobacter (60 to 65°C). The increased relative abundance of Coprothermobacter with increased temperature corresponding with a shift to syntrophic acetate oxidation identified this as a potentially key oxidiser. Methanosarcina likely acts as both a hydrogen utilising and acetoclastic methanogen at 55°C, and is replaced by Methanothermobacter as a hydrogen utiliser at higher temperatures.
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spelling pubmed-49738722016-08-18 High-rate, High Temperature Acetotrophic Methanogenesis Governed by a Three Population Consortium in Anaerobic Bioreactors Ho, Dang Jensen, Paul Gutierrez-Zamora, Maria-Luisa Beckmann, Sabrina Manefield, Mike Batstone, Damien PLoS One Research Article A combination of acetate oxidation and acetoclastic methanogenesis has been previously identified to enable high-rate methanogenesis at high temperatures (55 to 65°C), but this capability had not been linked to any key organisms. This study combined RNA–stable isotope probing on (13)C-labelled acetate and 16S amplicon sequencing to identify the active micro-organisms involved in high-rate methanogenesis. Active biomass was harvested from three bench-scale thermophilic bioreactors treating waste activated sludge at 55, 60 and 65°C, and fed with (13)-C labelled and (12)C-unlabelled acetate. Acetate uptake and cumulative methane production were determined and kinetic parameters were estimated using model-based analysis. Pyrosequencing performed on (13)C- enriched samples indicated that organisms accumulating labelled carbon were Coprothermobacter (all temperatures between 55 and 65°C), acetoclastic Methanosarcina (55 to 60°C) and hydrogenotrophic Methanothermobacter (60 to 65°C). The increased relative abundance of Coprothermobacter with increased temperature corresponding with a shift to syntrophic acetate oxidation identified this as a potentially key oxidiser. Methanosarcina likely acts as both a hydrogen utilising and acetoclastic methanogen at 55°C, and is replaced by Methanothermobacter as a hydrogen utiliser at higher temperatures. Public Library of Science 2016-08-04 /pmc/articles/PMC4973872/ /pubmed/27490246 http://dx.doi.org/10.1371/journal.pone.0159760 Text en © 2016 Ho et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Ho, Dang
Jensen, Paul
Gutierrez-Zamora, Maria-Luisa
Beckmann, Sabrina
Manefield, Mike
Batstone, Damien
High-rate, High Temperature Acetotrophic Methanogenesis Governed by a Three Population Consortium in Anaerobic Bioreactors
title High-rate, High Temperature Acetotrophic Methanogenesis Governed by a Three Population Consortium in Anaerobic Bioreactors
title_full High-rate, High Temperature Acetotrophic Methanogenesis Governed by a Three Population Consortium in Anaerobic Bioreactors
title_fullStr High-rate, High Temperature Acetotrophic Methanogenesis Governed by a Three Population Consortium in Anaerobic Bioreactors
title_full_unstemmed High-rate, High Temperature Acetotrophic Methanogenesis Governed by a Three Population Consortium in Anaerobic Bioreactors
title_short High-rate, High Temperature Acetotrophic Methanogenesis Governed by a Three Population Consortium in Anaerobic Bioreactors
title_sort high-rate, high temperature acetotrophic methanogenesis governed by a three population consortium in anaerobic bioreactors
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4973872/
https://www.ncbi.nlm.nih.gov/pubmed/27490246
http://dx.doi.org/10.1371/journal.pone.0159760
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