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The Metabolic Responses to L-Glutamine of Livers from Rats with Diabetes Types 1 and 2
There are several claims about the beneficial effects of supplementing L-glutamine to both type 1 and type 2 diabetes. The purpose of the present study was to provide detailed knowledge about the fate of this amino acid in the liver, the first organ that receives the compound when ingested orally. T...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4973899/ https://www.ncbi.nlm.nih.gov/pubmed/27490892 http://dx.doi.org/10.1371/journal.pone.0160067 |
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author | Comar, Jurandir Fernando de Oliveira, Denise Silva Bracht, Livia Kemmelmeier, Fumie Suzuki Peralta, Rosane Marina Bracht, Adelar |
author_facet | Comar, Jurandir Fernando de Oliveira, Denise Silva Bracht, Livia Kemmelmeier, Fumie Suzuki Peralta, Rosane Marina Bracht, Adelar |
author_sort | Comar, Jurandir Fernando |
collection | PubMed |
description | There are several claims about the beneficial effects of supplementing L-glutamine to both type 1 and type 2 diabetes. The purpose of the present study was to provide detailed knowledge about the fate of this amino acid in the liver, the first organ that receives the compound when ingested orally. The study was done using the isolated perfused rat liver, an experimental system that preserves the microcirculation of the organ and that allows to measured several parameters during steady-state and pre steady-state conditions. L-Glutamine was infused in the portal vein (5 mM) and several parameters were monitored. Livers from type 1 diabetic rats showed an accelerated response to L-glutamine infusion. In consequence of this accelerated response livers from type 1 diabetic rats presented higher rates of ammonia, urea, glucose and lactate output during the first 25–30 minutes following L-glutamine infusion. As steady-state conditions approached, however, the difference between type 1 diabetes and control livers tended to disappear. Measurement of the glycogen content over a period of 100 minutes revealed that, excepting the initial phase of the L-glutamine infusion, the increased glucose output in livers from type 1 diabetic rats was mainly due to accelerated glycogenolysis. Livers from type 2 diabetic rats behaved similarly to control livers with no accelerated glucose output but with increased L-alanine production. L-Alanine is important for the pancreatic β-cells and from this point of view the oral intake of L-glutamine can be regarded as beneficial. Furthermore, the lack of increased glucose output in livers from type 2 diabetic rats is consistent with observations that even daily L-glutamine doses of 30 g do not increase the glycemic levels in well controlled type 2 diabetes patients. |
format | Online Article Text |
id | pubmed-4973899 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-49738992016-08-18 The Metabolic Responses to L-Glutamine of Livers from Rats with Diabetes Types 1 and 2 Comar, Jurandir Fernando de Oliveira, Denise Silva Bracht, Livia Kemmelmeier, Fumie Suzuki Peralta, Rosane Marina Bracht, Adelar PLoS One Research Article There are several claims about the beneficial effects of supplementing L-glutamine to both type 1 and type 2 diabetes. The purpose of the present study was to provide detailed knowledge about the fate of this amino acid in the liver, the first organ that receives the compound when ingested orally. The study was done using the isolated perfused rat liver, an experimental system that preserves the microcirculation of the organ and that allows to measured several parameters during steady-state and pre steady-state conditions. L-Glutamine was infused in the portal vein (5 mM) and several parameters were monitored. Livers from type 1 diabetic rats showed an accelerated response to L-glutamine infusion. In consequence of this accelerated response livers from type 1 diabetic rats presented higher rates of ammonia, urea, glucose and lactate output during the first 25–30 minutes following L-glutamine infusion. As steady-state conditions approached, however, the difference between type 1 diabetes and control livers tended to disappear. Measurement of the glycogen content over a period of 100 minutes revealed that, excepting the initial phase of the L-glutamine infusion, the increased glucose output in livers from type 1 diabetic rats was mainly due to accelerated glycogenolysis. Livers from type 2 diabetic rats behaved similarly to control livers with no accelerated glucose output but with increased L-alanine production. L-Alanine is important for the pancreatic β-cells and from this point of view the oral intake of L-glutamine can be regarded as beneficial. Furthermore, the lack of increased glucose output in livers from type 2 diabetic rats is consistent with observations that even daily L-glutamine doses of 30 g do not increase the glycemic levels in well controlled type 2 diabetes patients. Public Library of Science 2016-08-04 /pmc/articles/PMC4973899/ /pubmed/27490892 http://dx.doi.org/10.1371/journal.pone.0160067 Text en © 2016 Comar et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Comar, Jurandir Fernando de Oliveira, Denise Silva Bracht, Livia Kemmelmeier, Fumie Suzuki Peralta, Rosane Marina Bracht, Adelar The Metabolic Responses to L-Glutamine of Livers from Rats with Diabetes Types 1 and 2 |
title | The Metabolic Responses to L-Glutamine of Livers from Rats with Diabetes Types 1 and 2 |
title_full | The Metabolic Responses to L-Glutamine of Livers from Rats with Diabetes Types 1 and 2 |
title_fullStr | The Metabolic Responses to L-Glutamine of Livers from Rats with Diabetes Types 1 and 2 |
title_full_unstemmed | The Metabolic Responses to L-Glutamine of Livers from Rats with Diabetes Types 1 and 2 |
title_short | The Metabolic Responses to L-Glutamine of Livers from Rats with Diabetes Types 1 and 2 |
title_sort | metabolic responses to l-glutamine of livers from rats with diabetes types 1 and 2 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4973899/ https://www.ncbi.nlm.nih.gov/pubmed/27490892 http://dx.doi.org/10.1371/journal.pone.0160067 |
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