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Gateway Vectors for Simultaneous Detection of Multiple Protein−Protein Interactions in Plant Cells Using Bimolecular Fluorescence Complementation
Bimolecular fluorescence complementation (BiFC) is widely used to detect protein—protein interactions, because it is technically simple, convenient, and can be adapted for use with conventional fluorescence microscopy. We previously constructed enhanced yellow fluorescent protein (EYFP)-based Gatewa...
Autores principales: | Kamigaki, Akane, Nito, Kazumasa, Hikino, Kazumi, Goto-Yamada, Shino, Nishimura, Mikio, Nakagawa, Tsuyoshi, Mano, Shoji |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4973907/ https://www.ncbi.nlm.nih.gov/pubmed/27490375 http://dx.doi.org/10.1371/journal.pone.0160717 |
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