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Optogenetic identification of a rapid-eye-movement (REM) sleep modulatory circuit in the hypothalamus
Rapid-Eye Movement (REM) sleep correlates with neuronal activity in the brainstem, basal forebrain and lateral hypothalamus (LH). LH melanin-concentrating hormone (MCH)-expressing neurons are active during sleep, however, their action on REM sleep remains unclear. Using optogenetic tools in newly-ge...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4974078/ https://www.ncbi.nlm.nih.gov/pubmed/24056699 http://dx.doi.org/10.1038/nn.3522 |
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author | Jego, Sonia Glasgow, Stephen D. Herrera, Carolina Gutierrez Ekstrand, Mats Reed, Sean J. Boyce, Richard Friedman, Jeffrey Burdakov, Denis Adamantidis, Antoine R. |
author_facet | Jego, Sonia Glasgow, Stephen D. Herrera, Carolina Gutierrez Ekstrand, Mats Reed, Sean J. Boyce, Richard Friedman, Jeffrey Burdakov, Denis Adamantidis, Antoine R. |
author_sort | Jego, Sonia |
collection | PubMed |
description | Rapid-Eye Movement (REM) sleep correlates with neuronal activity in the brainstem, basal forebrain and lateral hypothalamus (LH). LH melanin-concentrating hormone (MCH)-expressing neurons are active during sleep, however, their action on REM sleep remains unclear. Using optogenetic tools in newly-generated Tg(Pmch-Cre) mice, we found that acute activation of MCH neurons (ChETA, SSFO) at the onset of REM sleep extended the duration of REM, but not non-REM sleep episode. In contrast, their acute silencing (eNpHR3.0, ArchT) reduced the frequency and amplitude of hippocampal theta rhythm, without affecting REM sleep duration. In vitro activation of MCH neuron terminals induced GABA(A)-mediated inhibitory post-synaptic currents (IPSCs) in wake-promoting histaminergic neurons of the tuberomammillary nucleus (TMN), while in vivo activation of MCH neuron terminals in TMN or medial septum also prolonged REM sleep episodes. Collectively, these results suggest that activation of MCH neurons maintains REM sleep, possibly through inhibition of arousal circuits in the mammalian brain. |
format | Online Article Text |
id | pubmed-4974078 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
record_format | MEDLINE/PubMed |
spelling | pubmed-49740782016-08-04 Optogenetic identification of a rapid-eye-movement (REM) sleep modulatory circuit in the hypothalamus Jego, Sonia Glasgow, Stephen D. Herrera, Carolina Gutierrez Ekstrand, Mats Reed, Sean J. Boyce, Richard Friedman, Jeffrey Burdakov, Denis Adamantidis, Antoine R. Nat Neurosci Article Rapid-Eye Movement (REM) sleep correlates with neuronal activity in the brainstem, basal forebrain and lateral hypothalamus (LH). LH melanin-concentrating hormone (MCH)-expressing neurons are active during sleep, however, their action on REM sleep remains unclear. Using optogenetic tools in newly-generated Tg(Pmch-Cre) mice, we found that acute activation of MCH neurons (ChETA, SSFO) at the onset of REM sleep extended the duration of REM, but not non-REM sleep episode. In contrast, their acute silencing (eNpHR3.0, ArchT) reduced the frequency and amplitude of hippocampal theta rhythm, without affecting REM sleep duration. In vitro activation of MCH neuron terminals induced GABA(A)-mediated inhibitory post-synaptic currents (IPSCs) in wake-promoting histaminergic neurons of the tuberomammillary nucleus (TMN), while in vivo activation of MCH neuron terminals in TMN or medial septum also prolonged REM sleep episodes. Collectively, these results suggest that activation of MCH neurons maintains REM sleep, possibly through inhibition of arousal circuits in the mammalian brain. 2013-09-22 2013-11 /pmc/articles/PMC4974078/ /pubmed/24056699 http://dx.doi.org/10.1038/nn.3522 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Jego, Sonia Glasgow, Stephen D. Herrera, Carolina Gutierrez Ekstrand, Mats Reed, Sean J. Boyce, Richard Friedman, Jeffrey Burdakov, Denis Adamantidis, Antoine R. Optogenetic identification of a rapid-eye-movement (REM) sleep modulatory circuit in the hypothalamus |
title | Optogenetic identification of a rapid-eye-movement (REM) sleep modulatory circuit in the hypothalamus |
title_full | Optogenetic identification of a rapid-eye-movement (REM) sleep modulatory circuit in the hypothalamus |
title_fullStr | Optogenetic identification of a rapid-eye-movement (REM) sleep modulatory circuit in the hypothalamus |
title_full_unstemmed | Optogenetic identification of a rapid-eye-movement (REM) sleep modulatory circuit in the hypothalamus |
title_short | Optogenetic identification of a rapid-eye-movement (REM) sleep modulatory circuit in the hypothalamus |
title_sort | optogenetic identification of a rapid-eye-movement (rem) sleep modulatory circuit in the hypothalamus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4974078/ https://www.ncbi.nlm.nih.gov/pubmed/24056699 http://dx.doi.org/10.1038/nn.3522 |
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