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Optogenetic identification of a rapid-eye-movement (REM) sleep modulatory circuit in the hypothalamus

Rapid-Eye Movement (REM) sleep correlates with neuronal activity in the brainstem, basal forebrain and lateral hypothalamus (LH). LH melanin-concentrating hormone (MCH)-expressing neurons are active during sleep, however, their action on REM sleep remains unclear. Using optogenetic tools in newly-ge...

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Autores principales: Jego, Sonia, Glasgow, Stephen D., Herrera, Carolina Gutierrez, Ekstrand, Mats, Reed, Sean J., Boyce, Richard, Friedman, Jeffrey, Burdakov, Denis, Adamantidis, Antoine R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4974078/
https://www.ncbi.nlm.nih.gov/pubmed/24056699
http://dx.doi.org/10.1038/nn.3522
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author Jego, Sonia
Glasgow, Stephen D.
Herrera, Carolina Gutierrez
Ekstrand, Mats
Reed, Sean J.
Boyce, Richard
Friedman, Jeffrey
Burdakov, Denis
Adamantidis, Antoine R.
author_facet Jego, Sonia
Glasgow, Stephen D.
Herrera, Carolina Gutierrez
Ekstrand, Mats
Reed, Sean J.
Boyce, Richard
Friedman, Jeffrey
Burdakov, Denis
Adamantidis, Antoine R.
author_sort Jego, Sonia
collection PubMed
description Rapid-Eye Movement (REM) sleep correlates with neuronal activity in the brainstem, basal forebrain and lateral hypothalamus (LH). LH melanin-concentrating hormone (MCH)-expressing neurons are active during sleep, however, their action on REM sleep remains unclear. Using optogenetic tools in newly-generated Tg(Pmch-Cre) mice, we found that acute activation of MCH neurons (ChETA, SSFO) at the onset of REM sleep extended the duration of REM, but not non-REM sleep episode. In contrast, their acute silencing (eNpHR3.0, ArchT) reduced the frequency and amplitude of hippocampal theta rhythm, without affecting REM sleep duration. In vitro activation of MCH neuron terminals induced GABA(A)-mediated inhibitory post-synaptic currents (IPSCs) in wake-promoting histaminergic neurons of the tuberomammillary nucleus (TMN), while in vivo activation of MCH neuron terminals in TMN or medial septum also prolonged REM sleep episodes. Collectively, these results suggest that activation of MCH neurons maintains REM sleep, possibly through inhibition of arousal circuits in the mammalian brain.
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spelling pubmed-49740782016-08-04 Optogenetic identification of a rapid-eye-movement (REM) sleep modulatory circuit in the hypothalamus Jego, Sonia Glasgow, Stephen D. Herrera, Carolina Gutierrez Ekstrand, Mats Reed, Sean J. Boyce, Richard Friedman, Jeffrey Burdakov, Denis Adamantidis, Antoine R. Nat Neurosci Article Rapid-Eye Movement (REM) sleep correlates with neuronal activity in the brainstem, basal forebrain and lateral hypothalamus (LH). LH melanin-concentrating hormone (MCH)-expressing neurons are active during sleep, however, their action on REM sleep remains unclear. Using optogenetic tools in newly-generated Tg(Pmch-Cre) mice, we found that acute activation of MCH neurons (ChETA, SSFO) at the onset of REM sleep extended the duration of REM, but not non-REM sleep episode. In contrast, their acute silencing (eNpHR3.0, ArchT) reduced the frequency and amplitude of hippocampal theta rhythm, without affecting REM sleep duration. In vitro activation of MCH neuron terminals induced GABA(A)-mediated inhibitory post-synaptic currents (IPSCs) in wake-promoting histaminergic neurons of the tuberomammillary nucleus (TMN), while in vivo activation of MCH neuron terminals in TMN or medial septum also prolonged REM sleep episodes. Collectively, these results suggest that activation of MCH neurons maintains REM sleep, possibly through inhibition of arousal circuits in the mammalian brain. 2013-09-22 2013-11 /pmc/articles/PMC4974078/ /pubmed/24056699 http://dx.doi.org/10.1038/nn.3522 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Jego, Sonia
Glasgow, Stephen D.
Herrera, Carolina Gutierrez
Ekstrand, Mats
Reed, Sean J.
Boyce, Richard
Friedman, Jeffrey
Burdakov, Denis
Adamantidis, Antoine R.
Optogenetic identification of a rapid-eye-movement (REM) sleep modulatory circuit in the hypothalamus
title Optogenetic identification of a rapid-eye-movement (REM) sleep modulatory circuit in the hypothalamus
title_full Optogenetic identification of a rapid-eye-movement (REM) sleep modulatory circuit in the hypothalamus
title_fullStr Optogenetic identification of a rapid-eye-movement (REM) sleep modulatory circuit in the hypothalamus
title_full_unstemmed Optogenetic identification of a rapid-eye-movement (REM) sleep modulatory circuit in the hypothalamus
title_short Optogenetic identification of a rapid-eye-movement (REM) sleep modulatory circuit in the hypothalamus
title_sort optogenetic identification of a rapid-eye-movement (rem) sleep modulatory circuit in the hypothalamus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4974078/
https://www.ncbi.nlm.nih.gov/pubmed/24056699
http://dx.doi.org/10.1038/nn.3522
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