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Differentially expressed genes in preimplantation human embryos: potential candidate genes for blastocyst formation and implantation

PURPOSE: The aim of this study was to determine which genes and gene pathways are differentially expressed when comparing human blastocysts with cleavage-stage embryos. METHODS: We individually assessed gene expression in preimplantation human embryos at cleavage (n = 3) and blastocyst (n = 3) stage...

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Autores principales: Munch, Erika M., Sparks, Amy E., Gonzalez Bosquet, Jesus, Christenson, Lane K., Devor, Eric J., Van Voorhis, Bradley J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4974233/
https://www.ncbi.nlm.nih.gov/pubmed/27241529
http://dx.doi.org/10.1007/s10815-016-0745-x
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author Munch, Erika M.
Sparks, Amy E.
Gonzalez Bosquet, Jesus
Christenson, Lane K.
Devor, Eric J.
Van Voorhis, Bradley J.
author_facet Munch, Erika M.
Sparks, Amy E.
Gonzalez Bosquet, Jesus
Christenson, Lane K.
Devor, Eric J.
Van Voorhis, Bradley J.
author_sort Munch, Erika M.
collection PubMed
description PURPOSE: The aim of this study was to determine which genes and gene pathways are differentially expressed when comparing human blastocysts with cleavage-stage embryos. METHODS: We individually assessed gene expression in preimplantation human embryos at cleavage (n = 3) and blastocyst (n = 3) stages. Gene expression patterns were then validated in publically available datasets and then independently validated in vitro with additional human embryos using TaqMan gene expression assays. Immunolocalization studies were conducted to identify protein expression in intact blastocyst-stage embryos. RESULTS: Compared to cleavage-stage embryos, blastocyst-stage embryos differentially expressed 51 genes (p < 0.001), with overrepresentation in amoebiasis pathways and pathways in cancer. Of these 51 genes, 21 were found to be independently validated in a separate, publically available dataset, with a substantial agreement with our initial findings (κ = 0.8). In an independent set of cleavage- and blastocyst-stage embryos, we validated that six of eight tested genes were differentially expressed (p < 0.05) by RT-qPCR. Immunofluorescence studies documented the presence of two studied proteins in the trophectoderm of blastocyst-stage embryos. CONCLUSIONS: Differentially expressed genes may be implicated in the invasion and proliferation of the early embryo. Our research highlights specific genes that may be further studied for their role in the implantation process and additionally raises questions about localized gene and/or protein expression in the trophectoderm, which could affect protocols for, and interpretation of, trophectoderm biopsies performed in in vitro fertilization cycles. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10815-016-0745-x) contains supplementary material, which is available to authorized users.
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spelling pubmed-49742332016-08-17 Differentially expressed genes in preimplantation human embryos: potential candidate genes for blastocyst formation and implantation Munch, Erika M. Sparks, Amy E. Gonzalez Bosquet, Jesus Christenson, Lane K. Devor, Eric J. Van Voorhis, Bradley J. J Assist Reprod Genet Embryo Biology PURPOSE: The aim of this study was to determine which genes and gene pathways are differentially expressed when comparing human blastocysts with cleavage-stage embryos. METHODS: We individually assessed gene expression in preimplantation human embryos at cleavage (n = 3) and blastocyst (n = 3) stages. Gene expression patterns were then validated in publically available datasets and then independently validated in vitro with additional human embryos using TaqMan gene expression assays. Immunolocalization studies were conducted to identify protein expression in intact blastocyst-stage embryos. RESULTS: Compared to cleavage-stage embryos, blastocyst-stage embryos differentially expressed 51 genes (p < 0.001), with overrepresentation in amoebiasis pathways and pathways in cancer. Of these 51 genes, 21 were found to be independently validated in a separate, publically available dataset, with a substantial agreement with our initial findings (κ = 0.8). In an independent set of cleavage- and blastocyst-stage embryos, we validated that six of eight tested genes were differentially expressed (p < 0.05) by RT-qPCR. Immunofluorescence studies documented the presence of two studied proteins in the trophectoderm of blastocyst-stage embryos. CONCLUSIONS: Differentially expressed genes may be implicated in the invasion and proliferation of the early embryo. Our research highlights specific genes that may be further studied for their role in the implantation process and additionally raises questions about localized gene and/or protein expression in the trophectoderm, which could affect protocols for, and interpretation of, trophectoderm biopsies performed in in vitro fertilization cycles. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10815-016-0745-x) contains supplementary material, which is available to authorized users. Springer US 2016-05-30 2016-08 /pmc/articles/PMC4974233/ /pubmed/27241529 http://dx.doi.org/10.1007/s10815-016-0745-x Text en © The Author(s) 2016 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Embryo Biology
Munch, Erika M.
Sparks, Amy E.
Gonzalez Bosquet, Jesus
Christenson, Lane K.
Devor, Eric J.
Van Voorhis, Bradley J.
Differentially expressed genes in preimplantation human embryos: potential candidate genes for blastocyst formation and implantation
title Differentially expressed genes in preimplantation human embryos: potential candidate genes for blastocyst formation and implantation
title_full Differentially expressed genes in preimplantation human embryos: potential candidate genes for blastocyst formation and implantation
title_fullStr Differentially expressed genes in preimplantation human embryos: potential candidate genes for blastocyst formation and implantation
title_full_unstemmed Differentially expressed genes in preimplantation human embryos: potential candidate genes for blastocyst formation and implantation
title_short Differentially expressed genes in preimplantation human embryos: potential candidate genes for blastocyst formation and implantation
title_sort differentially expressed genes in preimplantation human embryos: potential candidate genes for blastocyst formation and implantation
topic Embryo Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4974233/
https://www.ncbi.nlm.nih.gov/pubmed/27241529
http://dx.doi.org/10.1007/s10815-016-0745-x
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