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Two-photon excited fluorescence of intrinsic fluorophores enables label-free assessment of adipose tissue function

Current methods for evaluating adipose tissue function are destructive or have low spatial resolution. These limit our ability to assess dynamic changes and heterogeneous responses that occur in healthy or diseased subjects, or during treatment. Here, we demonstrate that intrinsic two-photon excited...

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Autores principales: Alonzo, Carlo Amadeo, Karaliota, Sevasti, Pouli, Dimitra, Liu, Zhiyi, Karalis, Katia P., Georgakoudi, Irene
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4974509/
https://www.ncbi.nlm.nih.gov/pubmed/27491409
http://dx.doi.org/10.1038/srep31012
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author Alonzo, Carlo Amadeo
Karaliota, Sevasti
Pouli, Dimitra
Liu, Zhiyi
Karalis, Katia P.
Georgakoudi, Irene
author_facet Alonzo, Carlo Amadeo
Karaliota, Sevasti
Pouli, Dimitra
Liu, Zhiyi
Karalis, Katia P.
Georgakoudi, Irene
author_sort Alonzo, Carlo Amadeo
collection PubMed
description Current methods for evaluating adipose tissue function are destructive or have low spatial resolution. These limit our ability to assess dynamic changes and heterogeneous responses that occur in healthy or diseased subjects, or during treatment. Here, we demonstrate that intrinsic two-photon excited fluorescence enables functional imaging of adipocyte metabolism with subcellular resolution. Steady-state and time-resolved fluorescence from intracellular metabolic co-factors and lipid droplets can distinguish the functional states of excised white, brown, and cold-induced beige fat. Similar optical changes are identified when white and brown fat are assessed in vivo. Therefore, these studies establish the potential of non-invasive, high resolution, endogenous contrast, two-photon imaging to identify distinct adipose tissue types, monitor their functional state, and characterize heterogeneity of induced responses.
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spelling pubmed-49745092016-08-12 Two-photon excited fluorescence of intrinsic fluorophores enables label-free assessment of adipose tissue function Alonzo, Carlo Amadeo Karaliota, Sevasti Pouli, Dimitra Liu, Zhiyi Karalis, Katia P. Georgakoudi, Irene Sci Rep Article Current methods for evaluating adipose tissue function are destructive or have low spatial resolution. These limit our ability to assess dynamic changes and heterogeneous responses that occur in healthy or diseased subjects, or during treatment. Here, we demonstrate that intrinsic two-photon excited fluorescence enables functional imaging of adipocyte metabolism with subcellular resolution. Steady-state and time-resolved fluorescence from intracellular metabolic co-factors and lipid droplets can distinguish the functional states of excised white, brown, and cold-induced beige fat. Similar optical changes are identified when white and brown fat are assessed in vivo. Therefore, these studies establish the potential of non-invasive, high resolution, endogenous contrast, two-photon imaging to identify distinct adipose tissue types, monitor their functional state, and characterize heterogeneity of induced responses. Nature Publishing Group 2016-08-05 /pmc/articles/PMC4974509/ /pubmed/27491409 http://dx.doi.org/10.1038/srep31012 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Alonzo, Carlo Amadeo
Karaliota, Sevasti
Pouli, Dimitra
Liu, Zhiyi
Karalis, Katia P.
Georgakoudi, Irene
Two-photon excited fluorescence of intrinsic fluorophores enables label-free assessment of adipose tissue function
title Two-photon excited fluorescence of intrinsic fluorophores enables label-free assessment of adipose tissue function
title_full Two-photon excited fluorescence of intrinsic fluorophores enables label-free assessment of adipose tissue function
title_fullStr Two-photon excited fluorescence of intrinsic fluorophores enables label-free assessment of adipose tissue function
title_full_unstemmed Two-photon excited fluorescence of intrinsic fluorophores enables label-free assessment of adipose tissue function
title_short Two-photon excited fluorescence of intrinsic fluorophores enables label-free assessment of adipose tissue function
title_sort two-photon excited fluorescence of intrinsic fluorophores enables label-free assessment of adipose tissue function
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4974509/
https://www.ncbi.nlm.nih.gov/pubmed/27491409
http://dx.doi.org/10.1038/srep31012
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