Development of Neutralization Assay Using an eGFP Chikungunya Virus

Chikungunya virus (CHIKV), a member of the Alphavirus genus, is an important human emerging/re-emerging pathogen. Currently, there are no effective antiviral drugs or vaccines against CHIKV infection. Herein, we construct an infectious clone of CHIKV and an eGFP reporter CHIKV (eGFP-CHIKV) with an i...

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Detalles Bibliográficos
Autores principales: Deng, Cheng-Lin, Liu, Si-Qing, Zhou, Dong-Gen, Xu, Lin-Lin, Li, Xiao-Dan, Zhang, Pan-Tao, Li, Peng-Hui, Ye, Han-Qing, Wei, Hong-Ping, Yuan, Zhi-Ming, Qin, Cheng-Feng, Zhang, Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4974516/
https://www.ncbi.nlm.nih.gov/pubmed/27367716
http://dx.doi.org/10.3390/v8070181
Descripción
Sumario:Chikungunya virus (CHIKV), a member of the Alphavirus genus, is an important human emerging/re-emerging pathogen. Currently, there are no effective antiviral drugs or vaccines against CHIKV infection. Herein, we construct an infectious clone of CHIKV and an eGFP reporter CHIKV (eGFP-CHIKV) with an isolated strain (assigned to Asian lineage) from CHIKV-infected patients. The eGFP-CHIKV reporter virus allows for direct visualization of viral replication through the levels of eGFP expression. Using a known CHIKV inhibitor, ribavirin, we confirmed that the eGFP-CHIKV reporter virus could be used to identify inhibitors against CHIKV. Importantly, we developed a novel and reliable eGFP-CHIKV reporter virus-based neutralization assay that could be used for rapid screening neutralizing antibodies against CHIKV.

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