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Cross-Species Antiviral Activity of Goose Interferons against Duck Plague Virus Is Related to Its Positive Self-Feedback Regulation and Subsequent Interferon Stimulated Genes Induction

Interferons are a group of antiviral cytokines acting as the first line of defense in the antiviral immunity. Here, we describe the antiviral activity of goose type I interferon (IFNα) and type II interferon (IFNγ) against duck plague virus (DPV). Recombinant goose IFNα and IFNγ proteins of approxim...

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Autores principales: Zhou, Hao, Chen, Shun, Zhou, Qin, Wei, Yunan, Wang, Mingshu, Jia, Renyong, Zhu, Dekang, Liu, Mafeng, Liu, Fei, Yang, Qiao, Wu, Ying, Sun, Kunfeng, Chen, Xiaoyue, Cheng, Anchun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4974530/
https://www.ncbi.nlm.nih.gov/pubmed/27438848
http://dx.doi.org/10.3390/v8070195
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author Zhou, Hao
Chen, Shun
Zhou, Qin
Wei, Yunan
Wang, Mingshu
Jia, Renyong
Zhu, Dekang
Liu, Mafeng
Liu, Fei
Yang, Qiao
Wu, Ying
Sun, Kunfeng
Chen, Xiaoyue
Cheng, Anchun
author_facet Zhou, Hao
Chen, Shun
Zhou, Qin
Wei, Yunan
Wang, Mingshu
Jia, Renyong
Zhu, Dekang
Liu, Mafeng
Liu, Fei
Yang, Qiao
Wu, Ying
Sun, Kunfeng
Chen, Xiaoyue
Cheng, Anchun
author_sort Zhou, Hao
collection PubMed
description Interferons are a group of antiviral cytokines acting as the first line of defense in the antiviral immunity. Here, we describe the antiviral activity of goose type I interferon (IFNα) and type II interferon (IFNγ) against duck plague virus (DPV). Recombinant goose IFNα and IFNγ proteins of approximately 20 kDa and 18 kDa, respectively, were expressed. Following DPV-enhanced green fluorescent protein (EGFP) infection of duck embryo fibroblast cells (DEFs) with IFNα and IFNγ pre-treatment, the number of viral gene copies decreased more than 100-fold, with viral titers dropping approximately 100-fold. Compared to the control, DPV-EGFP cell positivity was decreased by goose IFNα and IFNγ at 36 hpi (3.89%; 0.79%) and 48 hpi (17.05%; 5.58%). In accordance with interferon-stimulated genes being the “workhorse” of IFN activity, the expression of duck myxovirus resistance (Mx) and oligoadenylate synthetases-like (OASL) was significantly upregulated (p < 0.001) by IFN treatment for 24 h. Interestingly, duck cells and goose cells showed a similar trend of increased ISG expression after goose IFNα and IFNγ pretreatment. Another interesting observation is that the positive feedback regulation of type I IFN and type II IFN by goose IFNα and IFNγ was confirmed in waterfowl for the first time. These results suggest that the antiviral activities of goose IFNα and IFNγ can likely be attributed to the potency with which downstream genes are induced by interferon. These findings will contribute to our understanding of the functional significance of the interferon antiviral system in aquatic birds and to the development of interferon-based prophylactic and therapeutic approaches against viral disease.
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spelling pubmed-49745302016-08-08 Cross-Species Antiviral Activity of Goose Interferons against Duck Plague Virus Is Related to Its Positive Self-Feedback Regulation and Subsequent Interferon Stimulated Genes Induction Zhou, Hao Chen, Shun Zhou, Qin Wei, Yunan Wang, Mingshu Jia, Renyong Zhu, Dekang Liu, Mafeng Liu, Fei Yang, Qiao Wu, Ying Sun, Kunfeng Chen, Xiaoyue Cheng, Anchun Viruses Article Interferons are a group of antiviral cytokines acting as the first line of defense in the antiviral immunity. Here, we describe the antiviral activity of goose type I interferon (IFNα) and type II interferon (IFNγ) against duck plague virus (DPV). Recombinant goose IFNα and IFNγ proteins of approximately 20 kDa and 18 kDa, respectively, were expressed. Following DPV-enhanced green fluorescent protein (EGFP) infection of duck embryo fibroblast cells (DEFs) with IFNα and IFNγ pre-treatment, the number of viral gene copies decreased more than 100-fold, with viral titers dropping approximately 100-fold. Compared to the control, DPV-EGFP cell positivity was decreased by goose IFNα and IFNγ at 36 hpi (3.89%; 0.79%) and 48 hpi (17.05%; 5.58%). In accordance with interferon-stimulated genes being the “workhorse” of IFN activity, the expression of duck myxovirus resistance (Mx) and oligoadenylate synthetases-like (OASL) was significantly upregulated (p < 0.001) by IFN treatment for 24 h. Interestingly, duck cells and goose cells showed a similar trend of increased ISG expression after goose IFNα and IFNγ pretreatment. Another interesting observation is that the positive feedback regulation of type I IFN and type II IFN by goose IFNα and IFNγ was confirmed in waterfowl for the first time. These results suggest that the antiviral activities of goose IFNα and IFNγ can likely be attributed to the potency with which downstream genes are induced by interferon. These findings will contribute to our understanding of the functional significance of the interferon antiviral system in aquatic birds and to the development of interferon-based prophylactic and therapeutic approaches against viral disease. MDPI 2016-07-18 /pmc/articles/PMC4974530/ /pubmed/27438848 http://dx.doi.org/10.3390/v8070195 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhou, Hao
Chen, Shun
Zhou, Qin
Wei, Yunan
Wang, Mingshu
Jia, Renyong
Zhu, Dekang
Liu, Mafeng
Liu, Fei
Yang, Qiao
Wu, Ying
Sun, Kunfeng
Chen, Xiaoyue
Cheng, Anchun
Cross-Species Antiviral Activity of Goose Interferons against Duck Plague Virus Is Related to Its Positive Self-Feedback Regulation and Subsequent Interferon Stimulated Genes Induction
title Cross-Species Antiviral Activity of Goose Interferons against Duck Plague Virus Is Related to Its Positive Self-Feedback Regulation and Subsequent Interferon Stimulated Genes Induction
title_full Cross-Species Antiviral Activity of Goose Interferons against Duck Plague Virus Is Related to Its Positive Self-Feedback Regulation and Subsequent Interferon Stimulated Genes Induction
title_fullStr Cross-Species Antiviral Activity of Goose Interferons against Duck Plague Virus Is Related to Its Positive Self-Feedback Regulation and Subsequent Interferon Stimulated Genes Induction
title_full_unstemmed Cross-Species Antiviral Activity of Goose Interferons against Duck Plague Virus Is Related to Its Positive Self-Feedback Regulation and Subsequent Interferon Stimulated Genes Induction
title_short Cross-Species Antiviral Activity of Goose Interferons against Duck Plague Virus Is Related to Its Positive Self-Feedback Regulation and Subsequent Interferon Stimulated Genes Induction
title_sort cross-species antiviral activity of goose interferons against duck plague virus is related to its positive self-feedback regulation and subsequent interferon stimulated genes induction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4974530/
https://www.ncbi.nlm.nih.gov/pubmed/27438848
http://dx.doi.org/10.3390/v8070195
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