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Distribution of proteins within different compartments of tendon varies according to tendon type

Although the predominant function of all tendons is to transfer force from muscle to bone and position the limbs, some tendons additionally function as energy stores, reducing the energetic cost of locomotion. To maximise energy storage and return, energy‐storing tendons need to be more extensible a...

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Autores principales: Thorpe, Chavaunne T., Karunaseelan, Kabelan J., Ng Chieng Hin, Jade, Riley, Graham P., Birch, Helen L., Clegg, Peter D., Screen, Hazel R. C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4974547/
https://www.ncbi.nlm.nih.gov/pubmed/27113131
http://dx.doi.org/10.1111/joa.12485
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author Thorpe, Chavaunne T.
Karunaseelan, Kabelan J.
Ng Chieng Hin, Jade
Riley, Graham P.
Birch, Helen L.
Clegg, Peter D.
Screen, Hazel R. C.
author_facet Thorpe, Chavaunne T.
Karunaseelan, Kabelan J.
Ng Chieng Hin, Jade
Riley, Graham P.
Birch, Helen L.
Clegg, Peter D.
Screen, Hazel R. C.
author_sort Thorpe, Chavaunne T.
collection PubMed
description Although the predominant function of all tendons is to transfer force from muscle to bone and position the limbs, some tendons additionally function as energy stores, reducing the energetic cost of locomotion. To maximise energy storage and return, energy‐storing tendons need to be more extensible and elastic than tendons with a purely positional function. These properties are conferred in part by a specialisation of a specific compartment of the tendon, the interfascicular matrix, which enables sliding and recoil between adjacent fascicles. However, the composition of the interfascicular matrix is poorly characterised and we therefore tested the hypothesis that the distribution of elastin and proteoglycans differs between energy‐storing and positional tendons, and that protein distribution varies between the fascicular matrix and the interfascicular matrix, with localisation of elastin and lubricin to the interfascicular matrix. Protein distribution in the energy‐storing equine superficial digital flexor tendon and positional common digital extensor tendon was assessed using histology and immunohistochemistry. The results support the hypothesis, demonstrating enrichment of lubricin in the interfascicular matrix in both tendon types, where it is likely to facilitate interfascicular sliding. Elastin was also localised to the interfascicular matrix, specifically in the energy‐storing superficial digital flexor tendon, which may account for the greater elasticity of the interfascicular matrix in this tendon. A differential distribution of proteoglycans was identified between tendon types and regions, which may indicate a distinct role for each of these proteins in tendon. These data provide important advances into fully characterising structure–function relationships within tendon.
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spelling pubmed-49745472016-10-15 Distribution of proteins within different compartments of tendon varies according to tendon type Thorpe, Chavaunne T. Karunaseelan, Kabelan J. Ng Chieng Hin, Jade Riley, Graham P. Birch, Helen L. Clegg, Peter D. Screen, Hazel R. C. J Anat Original Articles Although the predominant function of all tendons is to transfer force from muscle to bone and position the limbs, some tendons additionally function as energy stores, reducing the energetic cost of locomotion. To maximise energy storage and return, energy‐storing tendons need to be more extensible and elastic than tendons with a purely positional function. These properties are conferred in part by a specialisation of a specific compartment of the tendon, the interfascicular matrix, which enables sliding and recoil between adjacent fascicles. However, the composition of the interfascicular matrix is poorly characterised and we therefore tested the hypothesis that the distribution of elastin and proteoglycans differs between energy‐storing and positional tendons, and that protein distribution varies between the fascicular matrix and the interfascicular matrix, with localisation of elastin and lubricin to the interfascicular matrix. Protein distribution in the energy‐storing equine superficial digital flexor tendon and positional common digital extensor tendon was assessed using histology and immunohistochemistry. The results support the hypothesis, demonstrating enrichment of lubricin in the interfascicular matrix in both tendon types, where it is likely to facilitate interfascicular sliding. Elastin was also localised to the interfascicular matrix, specifically in the energy‐storing superficial digital flexor tendon, which may account for the greater elasticity of the interfascicular matrix in this tendon. A differential distribution of proteoglycans was identified between tendon types and regions, which may indicate a distinct role for each of these proteins in tendon. These data provide important advances into fully characterising structure–function relationships within tendon. John Wiley and Sons Inc. 2016-04-25 2016-09 /pmc/articles/PMC4974547/ /pubmed/27113131 http://dx.doi.org/10.1111/joa.12485 Text en © 2016 The Authors. Journal of Anatomy published by John Wiley & Sons Ltd on behalf of Anatomical Society. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Thorpe, Chavaunne T.
Karunaseelan, Kabelan J.
Ng Chieng Hin, Jade
Riley, Graham P.
Birch, Helen L.
Clegg, Peter D.
Screen, Hazel R. C.
Distribution of proteins within different compartments of tendon varies according to tendon type
title Distribution of proteins within different compartments of tendon varies according to tendon type
title_full Distribution of proteins within different compartments of tendon varies according to tendon type
title_fullStr Distribution of proteins within different compartments of tendon varies according to tendon type
title_full_unstemmed Distribution of proteins within different compartments of tendon varies according to tendon type
title_short Distribution of proteins within different compartments of tendon varies according to tendon type
title_sort distribution of proteins within different compartments of tendon varies according to tendon type
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4974547/
https://www.ncbi.nlm.nih.gov/pubmed/27113131
http://dx.doi.org/10.1111/joa.12485
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