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Anti-inflammatory activity of aqueous extract and bioactive compounds identified from the fruits of Hancornia speciosa Gomes (Apocynaceae)
BACKGROUND: Hancornia speciosa Gomes (Apocynaceae), popularly known as “mangabeira,” has been used in folk medicine to treat inflammatory disorders, hypertension, dermatitis, diabetes, liver diseases and gastric disorders. Although the ethnobotany indicates that its fruits can be used for the treatm...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4974780/ https://www.ncbi.nlm.nih.gov/pubmed/27496015 http://dx.doi.org/10.1186/s12906-016-1259-x |
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author | Torres-Rêgo, Manoela Furtado, Allanny Alves Bitencourt, Mariana Angélica Oliveira Lima, Maira Conceição Jerônimo de Souza Andrade, Rafael Caetano Lisbôa Castro de Azevedo, Eduardo Pereira de Soares, Thaciane da Cunha Tomaz, José Carlos Lopes, Norberto Peporine da Silva-Júnior, Arnóbio Antônio Zucolotto, Silvana Maria Fernandes-Pedrosa, Matheus de Freitas |
author_facet | Torres-Rêgo, Manoela Furtado, Allanny Alves Bitencourt, Mariana Angélica Oliveira Lima, Maira Conceição Jerônimo de Souza Andrade, Rafael Caetano Lisbôa Castro de Azevedo, Eduardo Pereira de Soares, Thaciane da Cunha Tomaz, José Carlos Lopes, Norberto Peporine da Silva-Júnior, Arnóbio Antônio Zucolotto, Silvana Maria Fernandes-Pedrosa, Matheus de Freitas |
author_sort | Torres-Rêgo, Manoela |
collection | PubMed |
description | BACKGROUND: Hancornia speciosa Gomes (Apocynaceae), popularly known as “mangabeira,” has been used in folk medicine to treat inflammatory disorders, hypertension, dermatitis, diabetes, liver diseases and gastric disorders. Although the ethnobotany indicates that its fruits can be used for the treatment of ulcers and inflammatory disorders, only few studies have been conducted to prove such biological activities. This study investigated the anti-inflammatory properties of the aqueous extract of the fruits of H. speciosa Gomes as well as its bioactive compounds using in vivo experimental models. METHODS: The bioactive compounds were identified by High Performance Liquid Chromatography coupled with diode array detector (HPLC-DAD) and Liquid Chromatography coupled with Mass Spectrometry (LC-MS). The anti-inflammatory properties were investigated through in vivo tests, which comprised xylene-induced ear edema, carrageenan-induced peritonitis and zymosan-induced air pouch. The levels of IL-1β, IL-6, IL-12 and TNF-α were determined using ELISA. RESULTS: Rutin and chlorogenic acid were identified in the extract as the main secondary metabolites. In addition, the extract as well as rutin and chlorogenic acid significantly inhibited the xilol-induced ear edema and also reduced the cell migration in both carrageenan-induced peritonitis and zymosan-induced air pouch models. Reduced levels of cytokines were also observed. CONCLUSION: This is the first study that demonstrated the anti-inflammatory activity of the extract of H. speciosa fruits against different inflammatory agents in animal models, suggesting that its bioactive molecules, especially rutin and chlorogenic acid are, at least in part, responsible for such activity. These findings support the widespread use of Hancornia speciosa in popular medicine and demonstrate that its aqueous extract has therapeutical potential for the development of herbal drugs with anti-inflammatory properties. |
format | Online Article Text |
id | pubmed-4974780 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-49747802016-08-06 Anti-inflammatory activity of aqueous extract and bioactive compounds identified from the fruits of Hancornia speciosa Gomes (Apocynaceae) Torres-Rêgo, Manoela Furtado, Allanny Alves Bitencourt, Mariana Angélica Oliveira Lima, Maira Conceição Jerônimo de Souza Andrade, Rafael Caetano Lisbôa Castro de Azevedo, Eduardo Pereira de Soares, Thaciane da Cunha Tomaz, José Carlos Lopes, Norberto Peporine da Silva-Júnior, Arnóbio Antônio Zucolotto, Silvana Maria Fernandes-Pedrosa, Matheus de Freitas BMC Complement Altern Med Research Article BACKGROUND: Hancornia speciosa Gomes (Apocynaceae), popularly known as “mangabeira,” has been used in folk medicine to treat inflammatory disorders, hypertension, dermatitis, diabetes, liver diseases and gastric disorders. Although the ethnobotany indicates that its fruits can be used for the treatment of ulcers and inflammatory disorders, only few studies have been conducted to prove such biological activities. This study investigated the anti-inflammatory properties of the aqueous extract of the fruits of H. speciosa Gomes as well as its bioactive compounds using in vivo experimental models. METHODS: The bioactive compounds were identified by High Performance Liquid Chromatography coupled with diode array detector (HPLC-DAD) and Liquid Chromatography coupled with Mass Spectrometry (LC-MS). The anti-inflammatory properties were investigated through in vivo tests, which comprised xylene-induced ear edema, carrageenan-induced peritonitis and zymosan-induced air pouch. The levels of IL-1β, IL-6, IL-12 and TNF-α were determined using ELISA. RESULTS: Rutin and chlorogenic acid were identified in the extract as the main secondary metabolites. In addition, the extract as well as rutin and chlorogenic acid significantly inhibited the xilol-induced ear edema and also reduced the cell migration in both carrageenan-induced peritonitis and zymosan-induced air pouch models. Reduced levels of cytokines were also observed. CONCLUSION: This is the first study that demonstrated the anti-inflammatory activity of the extract of H. speciosa fruits against different inflammatory agents in animal models, suggesting that its bioactive molecules, especially rutin and chlorogenic acid are, at least in part, responsible for such activity. These findings support the widespread use of Hancornia speciosa in popular medicine and demonstrate that its aqueous extract has therapeutical potential for the development of herbal drugs with anti-inflammatory properties. BioMed Central 2016-08-05 /pmc/articles/PMC4974780/ /pubmed/27496015 http://dx.doi.org/10.1186/s12906-016-1259-x Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Torres-Rêgo, Manoela Furtado, Allanny Alves Bitencourt, Mariana Angélica Oliveira Lima, Maira Conceição Jerônimo de Souza Andrade, Rafael Caetano Lisbôa Castro de Azevedo, Eduardo Pereira de Soares, Thaciane da Cunha Tomaz, José Carlos Lopes, Norberto Peporine da Silva-Júnior, Arnóbio Antônio Zucolotto, Silvana Maria Fernandes-Pedrosa, Matheus de Freitas Anti-inflammatory activity of aqueous extract and bioactive compounds identified from the fruits of Hancornia speciosa Gomes (Apocynaceae) |
title | Anti-inflammatory activity of aqueous extract and bioactive compounds identified from the fruits of Hancornia speciosa Gomes (Apocynaceae) |
title_full | Anti-inflammatory activity of aqueous extract and bioactive compounds identified from the fruits of Hancornia speciosa Gomes (Apocynaceae) |
title_fullStr | Anti-inflammatory activity of aqueous extract and bioactive compounds identified from the fruits of Hancornia speciosa Gomes (Apocynaceae) |
title_full_unstemmed | Anti-inflammatory activity of aqueous extract and bioactive compounds identified from the fruits of Hancornia speciosa Gomes (Apocynaceae) |
title_short | Anti-inflammatory activity of aqueous extract and bioactive compounds identified from the fruits of Hancornia speciosa Gomes (Apocynaceae) |
title_sort | anti-inflammatory activity of aqueous extract and bioactive compounds identified from the fruits of hancornia speciosa gomes (apocynaceae) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4974780/ https://www.ncbi.nlm.nih.gov/pubmed/27496015 http://dx.doi.org/10.1186/s12906-016-1259-x |
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