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Characterization of Cells Isolated from Genetic and Trauma-Induced Heterotopic Ossification

Heterotopic ossification (HO) is the pathologic formation of bone separate from the normal skeleton. Although several models exist for studying HO, an understanding of the common in vitro properties of cells isolated from these models is lacking. We studied three separate animal models of HO includi...

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Autores principales: Agarwal, Shailesh, Drake, James, Qureshi, Ammar T., Loder, Shawn, Li, Shuli, Shigemori, Kay, Peterson, Jonathan, Cholok, David, Forsberg, Jonathan A., Mishina, Yuji, Davis, Thomas A., Levi, Benjamin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4975503/
https://www.ncbi.nlm.nih.gov/pubmed/27494521
http://dx.doi.org/10.1371/journal.pone.0156253
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author Agarwal, Shailesh
Drake, James
Qureshi, Ammar T.
Loder, Shawn
Li, Shuli
Shigemori, Kay
Peterson, Jonathan
Cholok, David
Forsberg, Jonathan A.
Mishina, Yuji
Davis, Thomas A.
Levi, Benjamin
author_facet Agarwal, Shailesh
Drake, James
Qureshi, Ammar T.
Loder, Shawn
Li, Shuli
Shigemori, Kay
Peterson, Jonathan
Cholok, David
Forsberg, Jonathan A.
Mishina, Yuji
Davis, Thomas A.
Levi, Benjamin
author_sort Agarwal, Shailesh
collection PubMed
description Heterotopic ossification (HO) is the pathologic formation of bone separate from the normal skeleton. Although several models exist for studying HO, an understanding of the common in vitro properties of cells isolated from these models is lacking. We studied three separate animal models of HO including two models of trauma-induced HO and one model of genetic HO, and human HO specimens, to characterize the properties of cells derived from tissue containing pre-and mature ectopic bone in relation to analogous mesenchymal cell populations or osteoblasts obtained from normal muscle tissue. We found that when cultured in vitro, cells isolated from the trauma sites in two distinct models exhibited increased osteogenic differentiation when compared to cells isolated from uninjured controls. Furthermore, osteoblasts isolated from heterotopic bone in a genetic model of HO also exhibited increased osteogenic differentiation when compared with normal osteoblasts. Finally, osteoblasts derived from mature heterotopic bone obtained from human patients exhibited increased osteogenic differentiation when compared with normal bone from the same patients. These findings demonstrate that across models, cells derived from tissues forming heterotopic ossification exhibit increased osteogenic differentiation when compared with either normal tissues or osteoblasts. These cell types can be used in the future for in vitro investigations for drug screening purposes.
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spelling pubmed-49755032016-08-25 Characterization of Cells Isolated from Genetic and Trauma-Induced Heterotopic Ossification Agarwal, Shailesh Drake, James Qureshi, Ammar T. Loder, Shawn Li, Shuli Shigemori, Kay Peterson, Jonathan Cholok, David Forsberg, Jonathan A. Mishina, Yuji Davis, Thomas A. Levi, Benjamin PLoS One Research Article Heterotopic ossification (HO) is the pathologic formation of bone separate from the normal skeleton. Although several models exist for studying HO, an understanding of the common in vitro properties of cells isolated from these models is lacking. We studied three separate animal models of HO including two models of trauma-induced HO and one model of genetic HO, and human HO specimens, to characterize the properties of cells derived from tissue containing pre-and mature ectopic bone in relation to analogous mesenchymal cell populations or osteoblasts obtained from normal muscle tissue. We found that when cultured in vitro, cells isolated from the trauma sites in two distinct models exhibited increased osteogenic differentiation when compared to cells isolated from uninjured controls. Furthermore, osteoblasts isolated from heterotopic bone in a genetic model of HO also exhibited increased osteogenic differentiation when compared with normal osteoblasts. Finally, osteoblasts derived from mature heterotopic bone obtained from human patients exhibited increased osteogenic differentiation when compared with normal bone from the same patients. These findings demonstrate that across models, cells derived from tissues forming heterotopic ossification exhibit increased osteogenic differentiation when compared with either normal tissues or osteoblasts. These cell types can be used in the future for in vitro investigations for drug screening purposes. Public Library of Science 2016-08-05 /pmc/articles/PMC4975503/ /pubmed/27494521 http://dx.doi.org/10.1371/journal.pone.0156253 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Agarwal, Shailesh
Drake, James
Qureshi, Ammar T.
Loder, Shawn
Li, Shuli
Shigemori, Kay
Peterson, Jonathan
Cholok, David
Forsberg, Jonathan A.
Mishina, Yuji
Davis, Thomas A.
Levi, Benjamin
Characterization of Cells Isolated from Genetic and Trauma-Induced Heterotopic Ossification
title Characterization of Cells Isolated from Genetic and Trauma-Induced Heterotopic Ossification
title_full Characterization of Cells Isolated from Genetic and Trauma-Induced Heterotopic Ossification
title_fullStr Characterization of Cells Isolated from Genetic and Trauma-Induced Heterotopic Ossification
title_full_unstemmed Characterization of Cells Isolated from Genetic and Trauma-Induced Heterotopic Ossification
title_short Characterization of Cells Isolated from Genetic and Trauma-Induced Heterotopic Ossification
title_sort characterization of cells isolated from genetic and trauma-induced heterotopic ossification
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4975503/
https://www.ncbi.nlm.nih.gov/pubmed/27494521
http://dx.doi.org/10.1371/journal.pone.0156253
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