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Combining isoflurane anesthesia with midazolam and butorphanol in rats

Representative inhalant anesthetic agent, isoflurane is commonly used during surgery in rats. However, isoflurane mediates relatively strong respiratory depression. In human and veterinary medicine, sedatives and analgesics are co-administered to complement the anesthetic action of inhalant anesthes...

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Autores principales: Tsukamoto, Atsushi, Uchida, Kaho, Maesato, Shizuka, Sato, Reiichiro, Kanai, Eiichi, Inomata, Tomo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Association for Laboratory Animal Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4976236/
https://www.ncbi.nlm.nih.gov/pubmed/26876437
http://dx.doi.org/10.1538/expanim.15-0113
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author Tsukamoto, Atsushi
Uchida, Kaho
Maesato, Shizuka
Sato, Reiichiro
Kanai, Eiichi
Inomata, Tomo
author_facet Tsukamoto, Atsushi
Uchida, Kaho
Maesato, Shizuka
Sato, Reiichiro
Kanai, Eiichi
Inomata, Tomo
author_sort Tsukamoto, Atsushi
collection PubMed
description Representative inhalant anesthetic agent, isoflurane is commonly used during surgery in rats. However, isoflurane mediates relatively strong respiratory depression. In human and veterinary medicine, sedatives and analgesics are co-administered to complement the anesthetic action of inhalant anesthesia. The present study aimed to establish the novel balanced anesthesia that combines midazolam and butorphanol with isoflurane (MBI) in rats. Male Sprague Dawley rats were divided into 2 groups, and administered either isoflurane monoanesthesia or isoflurane with midazolam (2.5 mg/kg, ip) and butorphanol (2.0 mg/kg, ip). The minimum alveolar concentration (MAC) in each group was evaluated. Induction and recovery times were measured in each group. Adverse reactions during induction were also recorded. In each group, vital signs were assessed for 1 h under 1.5×MAC of isoflurane. Instability of vital signs was assessed under each anesthesia by calculating coefficient of variance. Compared with isoflurane monoanesthesia, MBI anesthesia caused 32% MAC reduction (isoflurane monoanesthesia: 1.30 ± 0.09%, MBI 0.87 ± 0.08%, P<0.05). MB premedication mediated smooth sedating action with low incidence of adverse reactions such as urination and defecation. Isoflurane monoanesthsesia remarkably decreased respiratory rate and saturation O(2) (SPO(2)). In contrast, MBI anesthesia resulted in a relatively stable respiratory rate without decreases in SPO(2) during the anesthetic period. In summary, MB premedication is effective for attenuating respiratory depression induced by isoflurane, and achieving smooth induction. This anesthetic protocol serves as a novel option for appropriate anesthesia in rats.
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spelling pubmed-49762362016-08-09 Combining isoflurane anesthesia with midazolam and butorphanol in rats Tsukamoto, Atsushi Uchida, Kaho Maesato, Shizuka Sato, Reiichiro Kanai, Eiichi Inomata, Tomo Exp Anim Original Representative inhalant anesthetic agent, isoflurane is commonly used during surgery in rats. However, isoflurane mediates relatively strong respiratory depression. In human and veterinary medicine, sedatives and analgesics are co-administered to complement the anesthetic action of inhalant anesthesia. The present study aimed to establish the novel balanced anesthesia that combines midazolam and butorphanol with isoflurane (MBI) in rats. Male Sprague Dawley rats were divided into 2 groups, and administered either isoflurane monoanesthesia or isoflurane with midazolam (2.5 mg/kg, ip) and butorphanol (2.0 mg/kg, ip). The minimum alveolar concentration (MAC) in each group was evaluated. Induction and recovery times were measured in each group. Adverse reactions during induction were also recorded. In each group, vital signs were assessed for 1 h under 1.5×MAC of isoflurane. Instability of vital signs was assessed under each anesthesia by calculating coefficient of variance. Compared with isoflurane monoanesthesia, MBI anesthesia caused 32% MAC reduction (isoflurane monoanesthesia: 1.30 ± 0.09%, MBI 0.87 ± 0.08%, P<0.05). MB premedication mediated smooth sedating action with low incidence of adverse reactions such as urination and defecation. Isoflurane monoanesthsesia remarkably decreased respiratory rate and saturation O(2) (SPO(2)). In contrast, MBI anesthesia resulted in a relatively stable respiratory rate without decreases in SPO(2) during the anesthetic period. In summary, MB premedication is effective for attenuating respiratory depression induced by isoflurane, and achieving smooth induction. This anesthetic protocol serves as a novel option for appropriate anesthesia in rats. Japanese Association for Laboratory Animal Science 2016-02-12 2016 /pmc/articles/PMC4976236/ /pubmed/26876437 http://dx.doi.org/10.1538/expanim.15-0113 Text en ©2016 Japanese Association for Laboratory Animal Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Original
Tsukamoto, Atsushi
Uchida, Kaho
Maesato, Shizuka
Sato, Reiichiro
Kanai, Eiichi
Inomata, Tomo
Combining isoflurane anesthesia with midazolam and butorphanol in rats
title Combining isoflurane anesthesia with midazolam and butorphanol in rats
title_full Combining isoflurane anesthesia with midazolam and butorphanol in rats
title_fullStr Combining isoflurane anesthesia with midazolam and butorphanol in rats
title_full_unstemmed Combining isoflurane anesthesia with midazolam and butorphanol in rats
title_short Combining isoflurane anesthesia with midazolam and butorphanol in rats
title_sort combining isoflurane anesthesia with midazolam and butorphanol in rats
topic Original
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4976236/
https://www.ncbi.nlm.nih.gov/pubmed/26876437
http://dx.doi.org/10.1538/expanim.15-0113
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