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Identification, localization, and functional analysis of the homologues of mouse CABS1 protein in porcine testis

Previously, we have identified a calcium-binding protein that is specifically expressed in spermatids and localized to the flagella of the mature sperm in mouse, so-called mCABS1. However, the physiological roles of CABS1 in the male reproductive system have not been fully elucidated yet. In the cur...

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Autores principales: Shawki, Hossam H., Kigoshi, Takumi, Katoh, Yuki, Matsuda, Manabu, Ugboma, Chioma M., Takahashi, Satoru, Oishi, Hisashi, Kawashima, Akihiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Association for Laboratory Animal Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4976239/
https://www.ncbi.nlm.nih.gov/pubmed/26960363
http://dx.doi.org/10.1538/expanim.15-0104
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author Shawki, Hossam H.
Kigoshi, Takumi
Katoh, Yuki
Matsuda, Manabu
Ugboma, Chioma M.
Takahashi, Satoru
Oishi, Hisashi
Kawashima, Akihiro
author_facet Shawki, Hossam H.
Kigoshi, Takumi
Katoh, Yuki
Matsuda, Manabu
Ugboma, Chioma M.
Takahashi, Satoru
Oishi, Hisashi
Kawashima, Akihiro
author_sort Shawki, Hossam H.
collection PubMed
description Previously, we have identified a calcium-binding protein that is specifically expressed in spermatids and localized to the flagella of the mature sperm in mouse, so-called mCABS1. However, the physiological roles of CABS1 in the male reproductive system have not been fully elucidated yet. In the current study, we aimed to localize and clarify the role of CABS1 in porcine (pCABS1). We determined for the first time the full nucleotides sequence of pCABS1 mRNA. pCABS1 protein was detected on SDS-PAGE gel as two bands at 75 kDa and 70 kDa in adult porcine testis, whereas one band at 70 kDa in epididymal sperm. pCABS1 immunoreactivity in seminiferous tubules was detected in the elongated spermatids, and that in the epididymal sperm was found in the acrosome as well as flagellum. The immunoreactivity of pCABS1 in the acrosomai region disappeared during acrosome reaction. We also identified that pCABS1 has a transmembrane domain using computational prediction of the amino acids sequence. The treatment of porcine capacitated sperm with anti-pCABS1 antiserum significantly decreased acrosome reactions. These results suggest that pCABS1 plays an important role in controlling calcium ion signaling during the acrosome reaction.
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spelling pubmed-49762392016-08-09 Identification, localization, and functional analysis of the homologues of mouse CABS1 protein in porcine testis Shawki, Hossam H. Kigoshi, Takumi Katoh, Yuki Matsuda, Manabu Ugboma, Chioma M. Takahashi, Satoru Oishi, Hisashi Kawashima, Akihiro Exp Anim Original Previously, we have identified a calcium-binding protein that is specifically expressed in spermatids and localized to the flagella of the mature sperm in mouse, so-called mCABS1. However, the physiological roles of CABS1 in the male reproductive system have not been fully elucidated yet. In the current study, we aimed to localize and clarify the role of CABS1 in porcine (pCABS1). We determined for the first time the full nucleotides sequence of pCABS1 mRNA. pCABS1 protein was detected on SDS-PAGE gel as two bands at 75 kDa and 70 kDa in adult porcine testis, whereas one band at 70 kDa in epididymal sperm. pCABS1 immunoreactivity in seminiferous tubules was detected in the elongated spermatids, and that in the epididymal sperm was found in the acrosome as well as flagellum. The immunoreactivity of pCABS1 in the acrosomai region disappeared during acrosome reaction. We also identified that pCABS1 has a transmembrane domain using computational prediction of the amino acids sequence. The treatment of porcine capacitated sperm with anti-pCABS1 antiserum significantly decreased acrosome reactions. These results suggest that pCABS1 plays an important role in controlling calcium ion signaling during the acrosome reaction. Japanese Association for Laboratory Animal Science 2016-03-08 2016 /pmc/articles/PMC4976239/ /pubmed/26960363 http://dx.doi.org/10.1538/expanim.15-0104 Text en ©2016 Japanese Association for Laboratory Animal Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Original
Shawki, Hossam H.
Kigoshi, Takumi
Katoh, Yuki
Matsuda, Manabu
Ugboma, Chioma M.
Takahashi, Satoru
Oishi, Hisashi
Kawashima, Akihiro
Identification, localization, and functional analysis of the homologues of mouse CABS1 protein in porcine testis
title Identification, localization, and functional analysis of the homologues of mouse CABS1 protein in porcine testis
title_full Identification, localization, and functional analysis of the homologues of mouse CABS1 protein in porcine testis
title_fullStr Identification, localization, and functional analysis of the homologues of mouse CABS1 protein in porcine testis
title_full_unstemmed Identification, localization, and functional analysis of the homologues of mouse CABS1 protein in porcine testis
title_short Identification, localization, and functional analysis of the homologues of mouse CABS1 protein in porcine testis
title_sort identification, localization, and functional analysis of the homologues of mouse cabs1 protein in porcine testis
topic Original
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4976239/
https://www.ncbi.nlm.nih.gov/pubmed/26960363
http://dx.doi.org/10.1538/expanim.15-0104
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