Utilization of the CRISPR/Cas9 system for the efficient production of mutant mice using crRNA/tracrRNA with Cas9 nickase and FokI-dCas9

The CRISPR/Cas9 system is a powerful genome editing tool for the production of genetically modified animals. To produce mutant mice, chimeric single-guide RNA (sgRNA) is cloned in a plasmid vector and a mixture of sgRNA and Cas9 are microinjected into the fertilized eggs. An issue associated with ge...

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Detalles Bibliográficos
Autores principales: Terao, Miho, Tamano, Moe, Hara, Satoshi, Kato, Tomoko, Kinoshita, Masato, Takada, Shuji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Association for Laboratory Animal Science 2016
Materias:
Original
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4976241/
https://www.ncbi.nlm.nih.gov/pubmed/26972821
http://dx.doi.org/10.1538/expanim.15-0116

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